Paper · 2010 · http://www.idosi.org/wasj/wasj8(B%26GE)/15.pdf

Induction of callus through anther and ovule culture in upland cotton (Gossypium hirsutum L.).

Shabana Memon; Shahnawaz Mari; Adriano Mari; et al.

5
claims
10
verbatim quotes
7
citations
1
research areas
—
Not open access

Abstract

Abstract: Three cotton genoytypes Reshmi, Rehmani and TH-3/83 were studied for callus induction under two concentrations (3 mg/l and 4 mg/l) in four auxins viz. 2,4-D, IAA, IBA and Kinetin. Immature ovules and anthers of cotton varieties were utilized as explant material on Murashige and Skoog (MS) media The best callus induction was observed from the ovules rather than the anthers. A high percentage of regenerable callus was produced in Reshmi variety through immature ovules observed in the medium supplemented with 3mg/l. Rehmani followed Reshmi variety which produced 88 % callus with 3mg/l, whereas callus induction through immature anthers, the callus formation was less from both concentrations (3 mg/l and 4 mg/l). However, when the amount of 2, 4-D was increased in the medium the rate of callus formation was reduced. Key words: Missing

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ovules produced better callus induction than anthers.

Treatment
ovulese0
Outcome
callus inductione0
Direction
beste0
Comparator
antherse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The best callus induction was observed from the ovules rather than the anthers.”

✓ located verbatim in the source · abstract, characters 306–385 · show in text ↑
t0

“upland cotton”

✓ located verbatim in the source · title, characters 56–69 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Immature Reshmi ovules produced a high percentage of regenerable callus with 3mg/l supplementation.

Material
Reshmie0
Organ / stage
immature ovulese0
Treatment
medium supplemented with 3mg/le0
Outcome
regenerable calluse0
Direction
highe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“A high percentage of regenerable callus was produced in Reshmi variety through immature ovules observed in the medium supplemented with 3mg/l.”

✓ located verbatim in the source · abstract, characters 386–528 · show in text ↑
t0

“upland cotton”

✓ located verbatim in the source · title, characters 56–69 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rehmani produced 88 % callus with 3mg/l, following Reshmi.

Material
Rehmanie0
Treatment
3mg/le0
Outcome
calluse0
Value
88 %e0
Comparator
Reshmie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Rehmani followed Reshmi variety which produced 88 % callus with 3mg/l”

✓ located verbatim in the source · abstract, characters 529–598 · show in text ↑
t0

“upland cotton”

✓ located verbatim in the source · title, characters 56–69 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Callus formation from immature anthers was lower at both concentrations.

Organ / stage
immature antherse0
Treatment
3 mg/l and 4 mg/le0
Outcome
callus formatione0
Direction
lesse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“callus induction through immature anthers, the callus formation was less from both concentrations (3 mg/l and 4 mg/l).”

✓ located verbatim in the source · abstract, characters 608–726 · show in text ↑
t0

“upland cotton”

✓ located verbatim in the source · title, characters 56–69 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Increasing 2, 4-D in the medium reduced callus formation.

Treatment
2, 4-De0
Outcome
rate of callus formatione0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“when the amount of 2, 4-D was increased in the medium the rate of callus formation was reduced.”

✓ located verbatim in the source · abstract, characters 736–831 · show in text ↑
t0

“upland cotton”

✓ located verbatim in the source · title, characters 56–69 · show in text ↑

2,4-D · growth · Gossypium hirsutum (cotton)Positive and negative results

20 papers: 12 positive, 8 negative or no effect

7 papers in the same direction as this paper · 12 in the opposite direction

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.