Paper · 2015

Effect of Explants and Plant Growth Regulators on Callus Induction in Ricinus communis L

Khadiga G. Abd Elaleem; Magda Mohamed Ahmed; M Aboul Enein Khalid; et al.

9
claims
19
verbatim quotes
3
citations
1
research areas
—
Not open access

Abstract

The present research work aimed to shedding light on the effect of various plant growth regulator namely 2,4 dichlorophenoxy acetic acid (2,4-D) and �-Naphthalene Acetic Acid (NAA) on callus induction of ricinus commun. Callus culture were initiated from cotyledonary leaf and root segments explants from in vitro Ricinus communis L seedling . After four day of inoculation callusing was observed from cotyledonary leaf segment explants, after about one week from root segment explant. Auxins 2,4-D and NAA effecting in callus agitationbut 2.5 mg/L 2,4-D proved to be more effected for induction of callus in R. communis L. Individual treatment of NAA reveal low effectiveness for callus induction. Among all treatment media comprising 2,4 D (2.5 mg/l) showed superior response of callus degree (4 .0±0.0) with cotyledonary leaf segment explant and at this concentration, maximum amount of callus was observed whereas, root segment explants showed (2.7±0.19 ) degree of callus response in the same concentration . The callus from cotyledonary leaf segment explants was Light brown colour with compact nature in media fortified with 2,4 D while in media fortified with NAA callus was Light green colour with Friable nature. The callus from root segment explants was creamy to yellow colour with compact nature with the both tested auxin.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 9

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Cotyledonary leaf explants formed callus after four days.

Taxon
Ricinus communis LRicinus communist0
Organ / stage
cotyledonary leaf segment explantse0
Outcome
callusing was observede0
Value
four daye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“After four day of inoculation callusing was observed from cotyledonary leaf segment explants”

✓ located verbatim in the source · abstract, characters 344–436 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Root explants formed callus after about one week.

Taxon
Ricinus communis LRicinus communist0
Organ / stage
root segment explante0
Outcome
callusingt1
Value
about one weeke0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“after about one week from root segment explant”

✓ located verbatim in the source · abstract, characters 438–484 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑
t1

“callusing was observed from cotyledonary leaf segment explants”

✓ located verbatim in the source · abstract, characters 374–436 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

2.5 mg/L 2,4-D was more effective for callus induction.

Taxon
R. communis LRicinus communise0
Treatment
2.5 mg/L 2,4-De0
Outcome
induction of calluse0
Direction
more effectede0
Comparator
NAAe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“2.5 mg/L 2,4-D proved to be more effected for induction of callus in R. communis L.”

✓ located verbatim in the source · abstract, characters 540–623 · show in text ↑
e1

“Individual treatment of NAA reveal low effectiveness for callus induction.”

✓ located verbatim in the source · abstract, characters 624–698 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

NAA alone had low effectiveness for callus induction.

Taxon
Ricinus communis LRicinus communist0
Treatment
NAAe0
Outcome
callus inductione0
Direction
low effectivenesse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Individual treatment of NAA reveal low effectiveness for callus induction.”

✓ located verbatim in the source · abstract, characters 624–698 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cotyledonary leaf explants on 2.5 mg/l 2,4 D had the highest callus response degree, 4 .0±0.0.

Taxon
Ricinus communis LRicinus communist0
Organ / stage
cotyledonary leaf segment explante0
Treatment
2,4 D (2.5 mg/l)e0
Outcome
callus degreee0
Direction
superiore0
Value
4 .0±0.0e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“media comprising 2,4 D (2.5 mg/l) showed superior response of callus degree (4 .0±0.0) with cotyledonary leaf segment explant”

✓ located verbatim in the source · abstract, characters 719–844 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Root explants at the same concentration had callus response degree 2.7±0.19.

Taxon
Ricinus communis LRicinus communist0
Organ / stage
root segment explantse0
Outcome
degree of callus responsee0
Value
2.7±0.19e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“root segment explants showed (2.7±0.19 ) degree of callus response in the same concentration”

✓ located verbatim in the source · abstract, characters 919–1011 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Leaf explant callus under 2,4 D was light brown and compact.

Taxon
Ricinus communis LRicinus communist0
Organ / stage
cotyledonary leaf segment explantse0
Treatment
2,4 De0
Outcome
Light brown colour with compact naturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus from cotyledonary leaf segment explants was Light brown colour with compact nature in media fortified with 2,4 D”

✓ located verbatim in the source · abstract, characters 1018–1137 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Leaf explant callus under NAA was light green and friable.

Taxon
Ricinus communis LRicinus communist0
Treatment
NAAe0
Outcome
Light green colour with Friable naturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“in media fortified with NAA callus was Light green colour with Friable nature”

✓ located verbatim in the source · abstract, characters 1144–1221 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Root explant callus was creamy to yellow and compact with both auxins.

Taxon
Ricinus communis LRicinus communist0
Organ / stage
root segment explantse0
Treatment
both tested auxine0
Outcome
creamy to yellow colour with compact naturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The callus from root segment explants was creamy to yellow colour with compact nature with the both tested auxin.”

✓ located verbatim in the source · abstract, characters 1223–1336 · show in text ↑
t0

“Ricinus communis L”

✓ located verbatim in the source · title, characters 70–88 · show in text ↑

1-naphthaleneacetic acid · growth · Ricinus communis (castor bean)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.