Abstract
An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant. Seeds were surface sterilized with 0.1% (w/v) mercuric chloride and germinated in growth regulatorfree MS medium. Cotyledonary nodes were excised from 5-7 days old seedlings and were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP, Kin singly or in combination with NAA. Use of BAP at 3.0 mg.l -1 induced the highest frequency (85%) of shoot induction as well as maximum number of shoots per explant (12.56). Proliferated shoot clusters were elongated in 1.0 mg.l -1 BAP in combination with 0.25 mg.l -1 GA3 . For root induction, in vitro shoots were transferred to rooting media containing NAA or IBA. The highest rooting frequency (87.5%) as well as highest number of roots (10.5) was observed in MS medium supplemented with 1.0 mg.l -1 NAA. Regenerated plantlets were acclimatized successfully in the growth room for further development.
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Claims 6
Claim · model-written summary
BAP at 3.0 mg/l induced the highest shoot induction frequency, 85%.
- Taxon
- castor
Ricinus communise0 - Organ / stage
- cotyledonary nodese0
- Treatment
- BAP at 3.0 mg.l -1e1
- Outcome
- shoot inductione1
- Direction
- higheste1
- Value
- 85%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant”
e1“Use of BAP at 3.0 mg.l -1 induced the highest frequency (85%) of shoot induction as well as maximum number of shoots per explant (12.56)”
Claim · model-written summary
BAP at 3.0 mg/l gave the maximum number of shoots per explant, 12.56.
- Taxon
- castorRicinus communise0
- Organ / stage
- cotyledonary nodese0
- Treatment
- BAP at 3.0 mg.l -1e1
- Outcome
- number of shoots per explante1
- Direction
- maximume1
- Value
- 12.56e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant”
e1“Use of BAP at 3.0 mg.l -1 induced the highest frequency (85%) of shoot induction as well as maximum number of shoots per explant (12.56)”
Claim · model-written summary
Shoot clusters were elongated by 1.0 mg/l BAP with 0.25 mg/l GA3.
- Taxon
- castorRicinus communise0
- Organ / stage
- cotyledonary nodese0
- Treatment
- 1.0 mg.l -1 BAP in combination with 0.25 mg.l -1 GA3e2
- Outcome
- Proliferated shoot clusterse2
- Direction
- elongatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant”
e2“Proliferated shoot clusters were elongated in 1.0 mg.l -1 BAP in combination with 0.25 mg.l -1 GA3”
Claim · model-written summary
NAA at 1.0 mg/l gave the highest rooting frequency, 87.5%.
- Taxon
- castorRicinus communise0
- Treatment
- 1.0 mg.l -1 NAAe3
- Outcome
- rooting frequencye3
- Direction
- higheste3
- Value
- 87.5%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant”
e3“The highest rooting frequency (87.5%) as well as highest number of roots (10.5) was observed in MS medium supplemented with 1.0 mg.l -1 NAA”
Claim · model-written summary
NAA at 1.0 mg/l gave the highest number of roots, 10.5.
- Taxon
- castorRicinus communise0
- Treatment
- 1.0 mg.l -1 NAAe3
- Outcome
- number of rootse3
- Direction
- higheste3
- Value
- 10.5e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant”
e3“The highest rooting frequency (87.5%) as well as highest number of roots (10.5) was observed in MS medium supplemented with 1.0 mg.l -1 NAA”
Claim · model-written summary
Regenerated castor plantlets were acclimatized successfully in the growth room.
- Taxon
- castorRicinus communise0
- Outcome
- acclimatized successfullye4
- Setting
- growth roome4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant”
e4“Regenerated plantlets were acclimatized successfully in the growth room for further development”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.