Abstract
An high-frequency protocol for induction of callus from anther explants of Ricinus communis was described. When anther explants of R. communis was cultured directly onto medium containing 6-benzylaminopurine (BA) induced formation of only poor quality callus that had a low induction frequency of anther callus (10.67%). However, treating the anther explants with high concentrations (7.5-120 mg/L) of BA solution for short time periods (5-80 min) helped to improve the induction frequency and enhance the quality of the callus formation significantly. The best callus induction (41.25%) was observed when anther explants were treated with 15 mg/L BA solution for 10 min before being inoculated onto hormone-free Murashige and Skoog (MS) medium for 30 days. In order to further optimize the culture system, after treated with 15 mg/L BA for 10 min, anther explants were inoculated on the hormone-free MS medium contained concentrations of sodium nitroprusside (SNP). The results showed that SNP significantly promoted the response of callus induction, especially when 8 mg/L SNP was applied, the the highest percentage of callus induction (60.37%) were gained.
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Claims 5
Claim · model-written summary
Direct culture on BA medium yielded poor-quality callus with 10.67% induction.
- Taxon
- R. communis
Ricinus communise0 - Organ / stage
- anther explantse0
- Treatment
- medium containing 6-benzylaminopurine (BA)e0
- Outcome
- induction frequency of anther calluse0
- Direction
- lowe0
- Value
- 10.67%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When anther explants of R. communis was cultured directly onto medium containing 6-benzylaminopurine (BA) induced formation of only poor quality callus that had a low induction frequency of anther callus (10.67%)”
Claim · model-written summary
Brief exposure to high BA concentrations significantly improved callus induction frequency and quality.
- Taxon
- R. communisRicinus communise1
- Organ / stage
- anther explantse0
- Treatment
- high concentrations (7.5-120 mg/L) of BA solution for short time periods (5-80 min)e0
- Outcome
- induction frequency and enhance the quality of the callus formatione0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treating the anther explants with high concentrations (7.5-120 mg/L) of BA solution for short time periods (5-80 min) helped to improve the induction frequency and enhance the quality of the callus formation significantly”
e1“anther explants of R. communis”
Claim · model-written summary
A 15 mg/L BA pretreatment for 10 min produced 41.25% callus induction.
- Taxon
- R. communisRicinus communise1
- Organ / stage
- anther explantse0
- Treatment
- 15 mg/L BA solution for 10 mine0
- Outcome
- callus inductione0
- Direction
- beste0
- Value
- 41.25%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best callus induction (41.25%) was observed when anther explants were treated with 15 mg/L BA solution for 10 min before being inoculated onto hormone-free Murashige and Skoog (MS) medium for 30 days”
e1“anther explants of R. communis”
Claim · model-written summary
SNP significantly promoted callus induction after BA pretreatment.
- Taxon
- R. communisRicinus communise1
- Treatment
- SNPe0
- Outcome
- response of callus inductione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNP significantly promoted the response of callus induction”
e1“anther explants of R. communis”
Claim · model-written summary
An 8 mg/L SNP treatment gave the highest callus induction, 60.37%.
- Taxon
- R. communisRicinus communise1
- Treatment
- 8 mg/L SNPe0
- Outcome
- percentage of callus inductione0
- Direction
- higheste0
- Value
- 60.37%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“especially when 8 mg/L SNP was applied, the the highest percentage of callus induction (60.37%) were gained”
e1“anther explants of R. communis”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.