Paper · 2017 · International Journal of Agricultural Research

Nutrient Requirements for in vitro Propagation of Ricinus communis L. Mature Zygotic Embryos

G.C. Louis; C.U. Okafor; C.E.A. Okezie

10
claims
20
verbatim quotes
3
citations
3
research areas
✓
Open access

Abstract

Background and Objective: Ricinus communis (R. communis) is famous for its economic values and of great importance to researchers interested in the raising plantlets from hybrids. This study was carried out on the nutrient requirements for the in vitro propagation of R. communis employing three basal media using zygotic embryos as explants. Materials and Methods: Following the maintenance of asepsis, zygotic embryos were excised from mature seeds and cultured on the three basal media with 3% sucrose and 8 g LG 1 of agar. Plant growth regulators were not added to the media. This study was done to determine the most suitable basal medium for the growth of R. communis zygotic embryo. Results: The results obtained showed that the three basal media employed supported in vitro regeneration of the embryo explants. The highest mean shoot length (4.4500.231 cm), the highest mean root length (2.1900.262 cm), highest mean fresh weight (0.3650.032 g), highest mean leaf area (1.9990.189 cm 2 ), highest mean percent sprouting (91.6600.000) and highest mean number of roots (4.6000.163) were observed on Murashige and Skoog medium whereas the highest mean sprout rate (0.3300.000) was obtained on Murashige and Skoog and Gamborg B5 media. The embryo explants were able to develop into normal plantlets even in the absence of growth regulators. This may determine that endogenous hormones in the zygotic embryos were present at an optimal level to support regeneration. Conclusion: Results from this study indicated that Murashige and Skoog basal medium was the best basal medium for the in vitro propagation of R. communis zygotic embryos and for mass production.

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Claims 10

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

All three basal media supported in vitro regeneration of embryo explants.

Taxon
Ricinus communisRicinus communist0
Organ / stage
embryo explantse0
Treatment
three basal mediae0
Outcome
in vitro regeneratione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The results obtained showed that the three basal media employed supported in vitro regeneration of the embryo explants.”

✓ located verbatim in the source · abstract, characters 699–818 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Murashige and Skoog medium gave the highest mean shoot length, 4.4500.231 cm.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog mediume0
Outcome
mean shoot lengthe0
Direction
higheste0
Value
4.4500.231 cme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest mean shoot length (4.4500.231 cm), the highest mean root length (2.1900.262 cm), highest mean fresh weight (0.3650.032 g), highest mean leaf area (1.9990.189 cm 2 ), highest mean percent sprouting (91.6600.000) and highest mean number of roots (4.6000.163) were observed on Murashige and Skoog medium”

✓ located verbatim in the source · abstract, characters 819–1131 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Murashige and Skoog medium gave the highest mean root length, 2.1900.262 cm.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog mediume0
Outcome
mean root lengthe0
Direction
higheste0
Value
2.1900.262 cme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest mean shoot length (4.4500.231 cm), the highest mean root length (2.1900.262 cm), highest mean fresh weight (0.3650.032 g), highest mean leaf area (1.9990.189 cm 2 ), highest mean percent sprouting (91.6600.000) and highest mean number of roots (4.6000.163) were observed on Murashige and Skoog medium”

✓ located verbatim in the source · abstract, characters 819–1131 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Murashige and Skoog medium gave the highest mean fresh weight, 0.3650.032 g.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog mediume0
Outcome
mean fresh weighte0
Direction
higheste0
Value
0.3650.032 ge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“highest mean fresh weight (0.3650.032 g), highest mean leaf area (1.9990.189 cm 2 ), highest mean percent sprouting (91.6600.000) and highest mean number of roots (4.6000.163) were observed on Murashige and Skoog medium”

✓ located verbatim in the source · abstract, characters 912–1131 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Murashige and Skoog medium gave the highest mean leaf area, 1.9990.189 cm 2.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog mediume0
Outcome
mean leaf areae0
Direction
higheste0
Value
1.9990.189 cm 2e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“highest mean leaf area (1.9990.189 cm 2 ), highest mean percent sprouting (91.6600.000) and highest mean number of roots (4.6000.163) were observed on Murashige and Skoog medium”

✓ located verbatim in the source · abstract, characters 954–1131 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Murashige and Skoog medium gave the highest mean sprouting percentage, 91.6600.000.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog mediume0
Outcome
mean percent sproutinge0
Direction
higheste0
Value
91.6600.000e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“highest mean percent sprouting (91.6600.000) and highest mean number of roots (4.6000.163) were observed on Murashige and Skoog medium”

✓ located verbatim in the source · abstract, characters 997–1131 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Murashige and Skoog medium gave the highest mean root number, 4.6000.163.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog mediume0
Outcome
mean number of rootse0
Direction
higheste0
Value
4.6000.163e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“highest mean number of roots (4.6000.163) were observed on Murashige and Skoog medium”

✓ located verbatim in the source · abstract, characters 1046–1131 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Murashige and Skoog and Gamborg B5 media jointly gave the highest mean sprout rate, 0.3300.000.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog and Gamborg B5 mediae0
Outcome
mean sprout ratee0
Direction
higheste0
Value
0.3300.000e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest mean sprout rate (0.3300.000) was obtained on Murashige and Skoog and Gamborg B5 media.”

✓ located verbatim in the source · abstract, characters 1140–1239 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Embryo explants developed into normal plantlets without growth regulators.

Taxon
Ricinus communisRicinus communist0
Organ / stage
embryo explantse0
Outcome
develop into normal plantletse0
Qualifier
absence of growth regulatorse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The embryo explants were able to develop into normal plantlets even in the absence of growth regulators.”

✓ located verbatim in the source · abstract, characters 1240–1344 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors judged Murashige and Skoog basal medium best for embryo propagation and mass production.

Taxon
Ricinus communisRicinus communist0
Treatment
Murashige and Skoog basal mediume0
Outcome
best basal medium for the in vitro propagation of R. communis zygotic embryos and for mass productione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ricinus communis
Ricinus communis · TaxonDvellakat · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Murashige and Skoog basal medium was the best basal medium for the in vitro propagation of R. communis zygotic embryos and for mass production.”

✓ located verbatim in the source · abstract, characters 1521–1664 · show in text ↑
t0

“Ricinus communis L.”

✓ located verbatim in the source · title, characters 50–69 · show in text ↑

in vitro · growth · Ricinus communis (castor bean)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.