Abstract
A complete micropropagation protocol was developed for Pistacia vera cv. Ghazvini, an important rootstock in Pistachio orchards in Iran. In present study, the efficiency of a new medium called GNH (Garoosi, Nezami and Haddad) was investigated against some standard media. Different vitamins, calcium (Ca) sources and plant growth regulators (PGRs) were tested on in vitro shoot multiplication and root induction. Results indicated that mean number of shoots (4.25 ± 0.25), and productivity (69.87 ± 9.19 mm) increased significantly when GNH medium formed the basal medium, compared to the standard media inc luding Murashige and Skoog medium (MS), Juglans Medium (DKW), and McCown Woody Plant Medium (WPM). The most suitable concentration Ca and vitamin sources for shoot multiplication were 3.0 mM Ca gluconate and DKW - vitamins. The most suitable PGRs were a comb ination of 0.5 or 1.0 mg l - 1 BAP and 0.1 mg l - 1 IBA. The highest rooting parameters were obtained when 3.0 mM Ca gluconate or Fe - EDDHA (with 0.2 mM Fe) were incorporated into the GNH medium containing 2.0 mg l - 1 α - naphthalenacetic acid (NAA). Finally, near ly 70% of the plantlets survived acclimatization in the greenhouse. The results suggested the GNH medium (supplemented with Ca gluconate and DKW - vitamins), as a considerable and specific medium for the rapid micropropagation of Pistacia vera cv. Ghazvini.
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Claims 10
Claim · model-written summary
A complete micropropagation protocol was developed for Pistacia vera cv. Ghazvini.
- Taxon
- Pistacia vera
Pistacia verae0 - Material
- cv. Ghazvinie0
- Outcome
- complete micropropagation protocole0
- Setting
- Irane0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A complete micropropagation protocol was developed for Pistacia vera cv. Ghazvini, an important rootstock in Pistachio orchards in Iran.”
Claim · model-written summary
GNH medium significantly increased mean shoot number to 4.25 ± 0.25 compared with standard media.
- Taxon
- Pistacia veraPistacia verae1
- Material
- cv. Ghazvinie1
- Treatment
- GNH mediume0
- Outcome
- mean number of shootse0
- Direction
- increasede0
- Value
- 4.25 ± 0.25e0
- Comparator
- standard mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results indicated that mean number of shoots (4.25 ± 0.25), and productivity (69.87 ± 9.19 mm) increased significantly when GNH medium formed the basal medium, compared to the standard media inc luding Murashige and Skoog medium (MS), Juglans Medium (DKW), and McCown Woody Plant Medium (WPM).”
e1“developed for Pistacia vera cv. Ghazvini”
Claim · model-written summary
GNH medium significantly increased productivity to 69.87 ± 9.19 mm compared with standard media.
- Taxon
- Pistacia veraPistacia verat0
- Treatment
- GNH mediume0
- Outcome
- productivitye0
- Direction
- increasede0
- Value
- 69.87 ± 9.19 mme0
- Comparator
- standard mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results indicated that mean number of shoots (4.25 ± 0.25), and productivity (69.87 ± 9.19 mm) increased significantly when GNH medium formed the basal medium, compared to the standard media inc luding Murashige and Skoog medium (MS), Juglans Medium (DKW), and McCown Woody Plant Medium (WPM).”
t0“Pistacia vera”
Claim · model-written summary
3.0 mM Ca gluconate was the most suitable calcium source for shoot multiplication.
- Taxon
- Pistacia veraPistacia verat0
- Treatment
- 3.0 mM Ca gluconatee0
- Outcome
- shoot multiplicatione0
- Direction
- most suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most suitable concentration Ca and vitamin sources for shoot multiplication were 3.0 mM Ca gluconate and DKW - vitamins.”
t0“Pistacia vera”
Claim · model-written summary
DKW vitamins were most suitable for shoot multiplication.
- Taxon
- Pistacia veraPistacia verat0
- Treatment
- DKW - vitaminse0
- Outcome
- shoot multiplicatione0
- Direction
- most suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most suitable concentration Ca and vitamin sources for shoot multiplication were 3.0 mM Ca gluconate and DKW - vitamins.”
t0“Pistacia vera”
Claim · model-written summary
The most suitable PGRs combined 0.5 or 1.0 mg l - 1 BAP with 0.1 mg l - 1 IBA.
- Taxon
- Pistacia veraPistacia verat0
- Treatment
- 0.5 or 1.0 mg l - 1 BAP and 0.1 mg l - 1 IBAe0
- Outcome
- most suitablee0
- Direction
- most suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most suitable PGRs were a comb ination of 0.5 or 1.0 mg l - 1 BAP and 0.1 mg l - 1 IBA.”
t0“Pistacia vera”
Claim · model-written summary
Adding 3.0 mM Ca gluconate to GNH containing 2.0 mg l - 1 NAA produced the highest rooting parameters.
- Taxon
- Pistacia veraPistacia verat0
- Treatment
- 3.0 mM Ca gluconatee0
- Outcome
- rooting parameterse0
- Direction
- higheste0
- Qualifier
- GNH medium containing 2.0 mg l - 1 α - naphthalenacetic acid (NAA)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest rooting parameters were obtained when 3.0 mM Ca gluconate or Fe - EDDHA (with 0.2 mM Fe) were incorporated into the GNH medium containing 2.0 mg l - 1 α - naphthalenacetic acid (NAA).”
t0“Pistacia vera”
Claim · model-written summary
Fe - EDDHA with 0.2 mM Fe in GNH containing 2.0 mg l - 1 NAA produced the highest rooting parameters.
- Taxon
- Pistacia veraPistacia verat0
- Treatment
- Fe - EDDHA (with 0.2 mM Fe)e0
- Outcome
- rooting parameterse0
- Direction
- higheste0
- Qualifier
- GNH medium containing 2.0 mg l - 1 α - naphthalenacetic acid (NAA)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest rooting parameters were obtained when 3.0 mM Ca gluconate or Fe - EDDHA (with 0.2 mM Fe) were incorporated into the GNH medium containing 2.0 mg l - 1 α - naphthalenacetic acid (NAA).”
t0“Pistacia vera”
Claim · model-written summary
Nearly 70% of plantlets survived greenhouse acclimatization.
- Taxon
- Pistacia veraPistacia verat0
- Organ / stage
- plantletse0
- Outcome
- survived acclimatizatione0
- Value
- 70%e0
- Qualifier
- near lye0
- Setting
- greenhousee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Finally, near ly 70% of the plantlets survived acclimatization in the greenhouse.”
t0“Pistacia vera”
Claim · model-written summary
The authors recommend GNH supplemented with Ca gluconate and DKW vitamins for rapid micropropagation of Pistacia vera cv. Ghazvini.
- Taxon
- Pistacia veraPistacia verae0
- Material
- cv. Ghazvinie0
- Outcome
- GNH medium (supplemented with Ca gluconate and DKW - vitamins)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results suggested the GNH medium (supplemented with Ca gluconate and DKW - vitamins), as a considerable and specific medium for the rapid micropropagation of Pistacia vera cv. Ghazvini.”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.