Abstract
ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.
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Claims 4
Claim · model-written summary
ZmSTK2_USP is located on chromosome 4 and belongs to a serine/threonine kinase gene in maize.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize”
Claim · model-written summary
The upstream sequence contained cis-element motifs and two anther/pollen promoter element types.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA)”
e1“gene in maize”
Claim · model-written summary
The reporter was active in mature maize pollen grains and pollen tubes, but not other floral or vegetative tissues.
- Organ / stage
- mature pollen grains and pollen tubese0
- Gene
- proZmSTK2_USP-GUSe0
- Outcome
- GUS activitye0
- Qualifier
- not found in other floral and vegetative tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues”
t0“maize”
Claim · model-written summary
The authors identify proZmSTK2_USP as a pollen specific promoter active from middle pollen development through maturation.
- Organ / stage
- middle stage of pollen development until pollen maturatione0
- Gene
- proZmSTK2_USPe0
- Outcome
- pollen-specific activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation”
t0“maize”