Paper · 2016 · Acta fytotechnica et zootechnica/Acta fytotechnica et zootechnica

Physiological responses of genotypes soybean to simulated drought stress

Eleonóra Krivosudská; Angela Filová

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Open access · cc-by

Abstract

Received: 2016-11-23 | Accepted: 2016-04-14 | Available online: 2016-12-22 http://dx.doi.org/10.15414/afz.2016.19.04.157-162 The objective of this research was to investigate possible genetic variation in the sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying. The work confirmed that the selected physiological characteristics (RWC, osmotic potential, stress index and created nodules on roots) are good evaluating parameters for the determination of water stress in plant. In the floricultural year 2014 an experiment with four genetic resources of soybean was launched. Sowing of Maverick (USA), Drina (HRV), Nigra (SVK) and Polanka (CZK) genotypes was carried out in the containers of 15 l capacity. This stress had a negative impact on the physiological parameters. By comparing the RWC values, the decrease was more significant at the end of dehydration, which was monitored in Maverick and Drina genotypes using the Nitrazon inoculants and water stress effect. Inoculated stressed Nigra and Polanka genotypes have kept higher water content till the end of dehydration period. Also the proline accumulation was monitored during the water stress, whilst higher content of free proline reached of Maverick. More remarkable decrease of osmotic potential was again registered in a foreign Drina and Maverick genotypes in the inoculated variations. Nigra and Polanka genotypes responses not so significant in the given conditions. Key words: soybean, drought, stress, inoculation of Nitrazon References CANDRÁKOVÁ, E. and LÍŠKA, E. (2006) Vplyv teplotných a vlahových podmienok na tvorbu úrody hrachu siateho, Influence of Temperature and Water Conditions on Field Pea Yield Formation. Acta fytotechnica et zootechnica, vol. 9, no. 3, pp. 57-61. DOWNIE, J. A. (1998) Functions of rhizobial nodulation genes. In Spaink, H. P., Kondorosi A. and Hooykaas, P. J. J. (ed.), Rhizobiacee. Dordrecht: Kluwer Academic Publisher, pp. 387. FAROOQ, M. et. al. (2009) Plant drought stress: effects, mechanisms and management. Journal of Sustainable Agriculture, pp. 153–188. doi: http://dx.doi.org/10.1080/10440040802548555 FISCHER, R.A. and MAURER, R. (1978) Drought resistance in spring wheat cultivars. I. Grain yield response. Aust. J. Agric. Res., vol. 29, pp. 897–907. doi: http://dx.doi.org/10.1071/AR9780897 FREDERICK, J.R., CAMP, C.R. and BAUER, P.J. (2001) Drought-stress effects on branch and mainstem seed yield and yield components of determinate soybean. Crop Science, vol. 41, no. 3, pp. 759-763. doi: http://dx.doi.org/10.2135/cropsci2001 JAMES, A. T., LAWN R. J. and COOPER, M. (2008) Genotypic variation for drought stress response traits in soybean. I. Variation in soybean and wild Glycine spp. for epidermal conductance, osmotic potential, and relative water content. Australian Journal of Agricultural Research, vol. 59, no.7, pp. 656–669. doi: http://dx.doi.org/10.1071/AR07159 KU, Y. S., AU-YEUNG, W. K. and YUNG, Y. L. (2013) Drought Stress and Tolerance in Soybean. Chapter 10. http://dx.doi.org/ 10.5772/52945 LEPORT, L. et aL. (2003) Physiological responses of cool-season grain legumes to drought in the lowrainfall Mediterranean environment of south-western Australia. In: N.P. Saxena (Ed.), Management of Agricultural Drought: Agronomic and Genetic Options, pp. 163–172, Enfield: Science Publishers. MALHOTRA, R.S., SARKER, A. and SAXENA, M.C. (2004) Drought tolerance in chickpea and lentil-present status and future strategies. Challenges and Strategies for Dryland Agriculture, no. 32, pp. 257–273. MIRABELLLA, R., (2004) Role of ROP GTPases and NodFactor Signaling in Medicago Root Nodule Infection: Thesis. Wageningen: Wageningen University, 160p. PATTERSON, T.G. and LARNE, T.A. (1983) Nitrogen fixation by soybeans. Seasonal and cultivar effects, and comparison of estimates. Crop Science, vol. 23, no.3, pp. 488-492. doi: http://dx.doi.org/10.2135/cropsci1983.0011183X002300030012x RASAEI, B., GHOBADI, M.E. and AMIRI, M. K. (2013) Effect of osmotic potential on germination and seedling characteristics of soybean seeds. International Journal of Agriculture and Crop Sciences. vol., 5, no.11, 1265-1268. RICCARDI, L.,POLIGNANO, G.B. and DE GIOVANNI, C. (2001) Genotypic response of Faba bean to water stress. Euphytica, vol.118, no.1. pp. 39–46. doi: http://dx.doi.org/10.1023/A:1004078017159 SANCHOLI, G., MOBASSER, H. R. and FANAEI, H. R. (2015) Effect Inoculation of Soybean Cultivars with bacteria Rhizobium japonicum in Sistan. Biological Forum – An International Journal no.1, pp. 552-558. ISSN 0975-1130. SADEGHIPOUR, O. and ABBASI, S. (2012) Soybean response to drought and seed inoculation. World Applied Sciences Journal, vol.1, no.1, pp. 55-60. doi: http://dx.doi.org/10.1111/j.1467-6486.2004.00462.x VIRGINIA, S., PAGAN, M. and COOPER, M. (2012) Genetic Analysis of Relative Water Content (RWC) in Two Recombinant Inbred Line Populations of Soybean [Glycine max (L.) Merr.] Journal of Plant Genome Sciences, vol. 1, no. 2. doi: http://dx.doi.org/10.5147/jpgs.2012.0058

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Water stress had a negative impact on the physiological parameters of soybean.

Taxon
soybeanGlycine maxe1
Treatment
soil dryinge1
Outcome
physiological parameterse2
Direction
negative impacte2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”

✓ located verbatim in the source · abstract, characters 209–296 · show in text ↑
e2

“This stress had a negative impact on the physiological parameters”

✓ located verbatim in the source · abstract, characters 741–806 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Relative water content decreased more significantly at the end of dehydration in Maverick.

Taxon
soybeanGlycine maxe1
Material
Mavericke4
Treatment
dehydratione3
Outcome
RWC valuese3
Direction
decreasee3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”

✓ located verbatim in the source · abstract, characters 209–296 · show in text ↑
e3

“By comparing the RWC values, the decrease was more significant at the end of dehydration”

✓ located verbatim in the source · abstract, characters 808–896 · show in text ↑
e4

“which was monitored in Maverick”

✓ located verbatim in the source · abstract, characters 898–929 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculated and stressed Nigra and Polanka kept higher water content until the end of dehydration.

Taxon
soybeanGlycine maxe1
Material
Nigrae7
Treatment
Inoculated stressede6
Outcome
water contente5
Direction
highere5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”

✓ located verbatim in the source · abstract, characters 209–296 · show in text ↑
e5

“have kept higher water content till the end of dehydration period”

✓ located verbatim in the source · abstract, characters 1053–1118 · show in text ↑
e6

“Inoculated stressed”

✓ located verbatim in the source · abstract, characters 1005–1024 · show in text ↑
e7

“Nigra (SVK) and Polanka (CZK) genotypes”

✓ located verbatim in the source · abstract, characters 649–688 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Free proline content was higher in Maverick under water stress.

Taxon
soybeanGlycine maxe1
Material
Mavericke2
Treatment
water stresse2
Outcome
content of free prolinee2
Direction
highere2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”

✓ located verbatim in the source · abstract, characters 209–296 · show in text ↑
e2

“Also the proline accumulation was monitored during the water stress, whilst higher content of free proline reached of Maverick”

✓ located verbatim in the source · abstract, characters 1120–1246 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Osmotic potential decreased more remarkably in Drina under inoculated conditions.

Taxon
soybeanGlycine maxe1
Material
Drinae3
Treatment
inoculatede3
Outcome
osmotic potentiale2
Direction
decreasee2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”

✓ located verbatim in the source · abstract, characters 209–296 · show in text ↑
e2

“More remarkable decrease of osmotic potential”

✓ located verbatim in the source · abstract, characters 1248–1293 · show in text ↑
e3

“Drina and Maverick genotypes in the inoculated variations”

✓ located verbatim in the source · abstract, characters 1328–1385 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Responses of Nigra and Polanka were not significant under the given conditions.

Taxon
soybeanGlycine maxe1
Material
Nigrae7
Outcome
responsese2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”

✓ located verbatim in the source · abstract, characters 209–296 · show in text ↑
e7

“Nigra (SVK) and Polanka (CZK) genotypes”

✓ located verbatim in the source · abstract, characters 649–688 · show in text ↑
e2

“responses not so significant in the given conditions”

✓ located verbatim in the source · abstract, characters 1415–1467 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

RWC, osmotic potential, stress index and nodule number were good evaluating parameters for water stress in soybean.

Taxon
soybeanGlycine maxe1
Outcome
determination of water stresse2
Value
good evaluating parameterse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”

✓ located verbatim in the source · abstract, characters 209–296 · show in text ↑
e2

“The work confirmed that the selected physiological characteristics (RWC, osmotic potential, stress index and created nodules on roots) are good evaluating parameters for the determination of water stress in plant”

✓ located verbatim in the source · abstract, characters 298–510 · show in text ↑

inoculation · soil: physics · Glycine max (soybean)Results agree

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