Abstract
Like humans, viral diseases also affect plants. Of these, viruses belonging to the potyvirus genus are the most prolific. The potyvirus soybean mosaic virus (SMV) is an important pathogen of the crop plant soybean. SMV causes mosaic symptoms (yellow areas alternate with dark green areas on the leaves of the plant) and can affect yield by reducing seed quality. Few cultivars from soybean can resist different SMV strains. To understand soybean defense mechanisms to SMV, I identified soybean proteins that interact with the helper component protease (HC-Pro) of SMV, which also functions as the suppressor of host RNA silencing and thereby contributes to viral virulence. A genome wide yeast two hybrid screen identified two HC-Pro interactors; BRI1-associated receptor kinase 1 (BAK1) and ubiquitin conjugating enzyme 2 (UBC2). Interactions with HC-Pro were confirmed using bimolecular florescence complementation (BiFC), and co-immunoprecipitations (Co-IP) assays. HC-Pro showed co-localization with both BAK1 and UBC2 in planta. Six isoforms of BAK1 were identified in soybean (BAK1 a, b, c, d, e, and f). Functional analysis showed that silencing the gene encoding BAK1a resulted in breakdown of resistance derived from the resistance (R) locus Rsv1, against SMV. Consistent with the fact that BAK1 is well known regulator of plant basal immunity, soybean plants silenced for BAK1 exhibited enhanced susceptibility to the bacterial pathogen Pseudomonas syringae. BAK1, a receptor-like kinase, functions as a co-receptor in plant defense signaling as well as brassinosteroid-derived signaling during plant growth. My data indicates that HC-Pro is phosphorylated in the presence of BAK1 and this requires the T341 residue which regulates virus avirulence in Rsv1 plants. This is an important finding because although BAK1 is well known to phosphorylate BRI1 and other defense-related receptors, its involvement in phosphorylating pathogen-derived proteins has not been reported. My work raises the possibility that BAK1-derived phosphorylation of HC-Pro may be important to trigger Rsv1-mediated resistance against SMV.
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Claims 9
Claim · model-written summary
In a genome-wide yeast two-hybrid screen, two soybean proteins, BAK1 and UBC2, were identified as interactors of SMV HC-Pro.
- Taxon
- soybean
Glycine maxt0 - Interactor
- soybean mosaic virus
Soybean mosaic viruse0pathogen - Gene
- BRI1-associated receptor kinase 1 (BAK1) and ubiquitin conjugating enzyme 2 (UBC2)e1
- Outcome
- HC-Pro interactorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e0“The potyvirus soybean mosaic virus (SMV) is an important pathogen of the crop plant soybean”
e1“A genome wide yeast two hybrid screen identified two HC-Pro interactors; BRI1-associated receptor kinase 1 (BAK1) and ubiquitin conjugating enzyme 2 (UBC2)”
Claim · model-written summary
Interactions of HC-Pro with BAK1 and UBC2 were confirmed by BiFC and co-immunoprecipitation assays.
- Taxon
- soybeanGlycine maxt0
- Interactor
- soybean mosaic virusSoybean mosaic viruse0pathogen
- Outcome
- Interactions with HC-Proe2
- Qualifier
- confirmed using bimolecular florescence complementation (BiFC), and co-immunoprecipitations (Co-IP) assayse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e0“The potyvirus soybean mosaic virus (SMV) is an important pathogen of the crop plant soybean”
e2“Interactions with HC-Pro were confirmed using bimolecular florescence complementation (BiFC), and co-immunoprecipitations (Co-IP) assays”
Claim · model-written summary
HC-Pro co-localized with both BAK1 and UBC2 in planta.
- Taxon
- soybeanGlycine maxt0
- Interactor
- soybean mosaic virusSoybean mosaic viruse0pathogen
- Outcome
- co-localization with both BAK1 and UBC2e3
- Qualifier
- in plantae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e0“The potyvirus soybean mosaic virus (SMV) is an important pathogen of the crop plant soybean”
e3“HC-Pro showed co-localization with both BAK1 and UBC2 in planta”
Claim · model-written summary
Six BAK1 isoforms were identified in soybean.
- Taxon
- soybeanGlycine maxe4
- Gene
- BAK1e4
- Outcome
- isoforms of BAK1e4
- Value
- Sixe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Six isoforms of BAK1 were identified in soybean”
Claim · model-written summary
Silencing the gene encoding BAK1a broke down Rsv1-derived resistance to SMV in soybean.
- Taxon
- soybeanGlycine maxt0
- Treatment
- silencing the gene encoding BAK1ae5
- Interactor
- SMVe5pathogen
- Gene
- BAK1ae5
- Outcome
- resistance derived from the resistance (R) locus Rsv1e5
- Direction
- breakdowne5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e5“silencing the gene encoding BAK1a resulted in breakdown of resistance derived from the resistance (R) locus Rsv1, against SMV”
Claim · model-written summary
BAK1-silenced soybean plants showed enhanced susceptibility to Pseudomonas syringae.
- Taxon
- soybeanGlycine maxt0
- Treatment
- silenced for BAK1e6
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee6pathogen - Outcome
- susceptibilitye6
- Direction
- enhancede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e6“soybean plants silenced for BAK1 exhibited enhanced susceptibility to the bacterial pathogen Pseudomonas syringae”
Claim · model-written summary
HC-Pro is phosphorylated in the presence of BAK1, and this requires the T341 residue.
- Taxon
- soybeanGlycine maxt0
- Interactor
- soybean mosaic virusSoybean mosaic viruse0pathogen
- Gene
- HC-Proe7
- Outcome
- HC-Pro is phosphorylatede7
- Qualifier
- requires the T341 residuee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e0“The potyvirus soybean mosaic virus (SMV) is an important pathogen of the crop plant soybean”
e7“My data indicates that HC-Pro is phosphorylated in the presence of BAK1 and this requires the T341 residue which regulates virus avirulence in Rsv1 plants”
Claim · model-written summary
The T341 residue of HC-Pro regulates virus avirulence in Rsv1 plants.
- Taxon
- soybeanGlycine maxt0
- Material
- Rsv1 plantse7
- Outcome
- virus avirulencee7
- Qualifier
- T341 residuee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e7“this requires the T341 residue which regulates virus avirulence in Rsv1 plants”
Claim · model-written summary
BAK1-derived phosphorylation of HC-Pro may be important for triggering Rsv1-mediated resistance against SMV.
- Taxon
- soybeanGlycine maxt0
- Gene
- BAK1e7
- Outcome
- Rsv1-mediated resistance against SMVe7
- Qualifier
- may be importante7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rsv1 Mediated Resistance to SMV in Soybean”
e7“My work raises the possibility that BAK1-derived phosphorylation of HC-Pro may be important to trigger Rsv1-mediated resistance against SMV”
The same questions in other papers
loss of function · disease · Glycine max (soybean)Positive and negative results
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- negative
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See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.