Abstract
HSFA9 is a seed-specific transcription factor that in sunflower (Helianthus annuus) is involved in desiccation tolerance and longevity. Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness and accelerated the initial photosynthetic development. Plants overexpressing HSFA9 increased accumulation of carotenoids, chlorophyllide, and chlorophyll, and displayed earlier unfolding of the cotyledons. HSFA9 enhanced phytochrome-dependent light responses, as shown by an intensified hypocotyl length reduction after treatments with continuous far-red or red light. This observation indicated the involvement of at least two phytochromes: PHYA and PHYB. Reduced hypocotyl length under darkness did not depend on phytochrome photo-activation; this was inferred from the lack of effect observed using far-red light pulses applied before the dark treatment. HSFA9 increased the expression of genes that activate photomorphogenesis, including PHYA, PHYB, and HY5. HSFA9 might directly upregulate PHYA and indirectly affect PHYB transcription, as suggested by transient expression assays. Converse effects on gene expression, greening, and cotyledon unfolding were observed using a dominant-negative form of HSFA9, which was overexpressed under a seed-specific promoter. This work uncovers a novel transcriptional link, through HSFA9, between seed maturation and early photomorphogenesis. In all, our data suggest that HSFA9 enhances photomorphogenesis via early transcriptional effects that start in seeds under darkness.
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Claims 8
Claim · model-written summary
Overexpression of HSFA9 attenuated hypocotyl growth of tobacco seedlings under darkness.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- constitutive overexpression of HSFA9e1
- Outcome
- hypocotyl growth under darknesse1
- Direction
- attenuatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
Claim · model-written summary
Overexpression of HSFA9 increased accumulation of carotenoids, chlorophyllide and chlorophyll in tobacco seedlings.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overexpressing HSFA9e0
- Outcome
- accumulation of carotenoids, chlorophyllide, and chlorophylle0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
e0“Plants overexpressing HSFA9 increased accumulation of carotenoids, chlorophyllide, and chlorophyll”
Claim · model-written summary
Overexpression of HSFA9 led to earlier unfolding of the cotyledons in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overexpressing HSFA9e0
- Outcome
- unfolding of the cotyledonse2
- Direction
- earliere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
e0“Plants overexpressing HSFA9 increased accumulation of carotenoids, chlorophyllide, and chlorophyll”
e2“displayed earlier unfolding of the cotyledons”
Claim · model-written summary
HSFA9 intensified the hypocotyl length reduction of tobacco after continuous far-red or red light.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- continuous far-red or red lighte3
- Outcome
- hypocotyl length reductione3
- Direction
- intensifiede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
e3“HSFA9 enhanced phytochrome-dependent light responses, as shown by an intensified hypocotyl length reduction after treatments with continuous far-red or red light”
Claim · model-written summary
Reduced hypocotyl length under darkness in tobacco did not depend on phytochrome photo-activation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- phytochrome photo-activatione4
- Outcome
- hypocotyl length under darknesse4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
e4“Reduced hypocotyl length under darkness did not depend on phytochrome photo-activation”
Claim · model-written summary
HSFA9 increased the expression of PHYA, PHYB and HY5 genes in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- HSFA9e5
- Gene
- PHYA, PHYB, and HY5e5
- Outcome
- expression of genese5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
e5“HSFA9 increased the expression of genes that activate photomorphogenesis, including PHYA, PHYB, and HY5”
Claim · model-written summary
HSFA9 might directly upregulate PHYA and thereby indirectly affect PHYB transcription in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- PHYAe6
- Outcome
- PHYB transcriptione6
- Qualifier
- might directly upregulatee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
e6“HSFA9 might directly upregulate PHYA and indirectly affect PHYB transcription, as suggested by transient expression assays”
Claim · model-written summary
A dominant-negative form of HSFA9 produced converse effects on gene expression, greening and cotyledon unfolding in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- dominant-negative form of HSFA9e7
- Outcome
- gene expression, greening, and cotyledon unfoldinge7
- Direction
- Converse effectse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that the constitutive overexpression of HSFA9 in tobacco (Nicotiana tabacum) seedlings attenuated hypocotyl growth under darkness”
e7“Converse effects on gene expression, greening, and cotyledon unfolding were observed using a dominant-negative form of HSFA9”
The same questions in other papers
light · growth · Nicotiana tabacum (tobacco)Positive and negative results
28 papers in the same direction as this paper · 16 in the opposite direction · 2 reporting no difference from an alternative
- no effect
- no effect
- negative
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.