Paper · 2014 · Acta Horticulturae

ISOLATION AND EXPRESSION ANALYSIS OF THREE FLOWERING RELATED GENES OF CULTIVATED STRAWBERRY

Y.X. Liu; D.M. Zou; Z.H. Zhang; et al.

8
claims
16
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

Flowering is important for the production of strawberry, but its molecular mechanism is not clear now. To investigate the molecular mechanism of flowering induction in octoploid strawberry, we isolated and analyzed three important flowering related genes (FaLFY, FaAP1 and FaTFL1) from cultivated strawberry 'Huaji'. Partial sequences of these three genes were isolated with PCR and their expression roles in different tissues and organs of strawberry were detected. The deduced amino acids of FaLFY showed the highest degree of similarity to its homologue in Rosa lucieae (93%), FaAP1 showed the highest degree of similarity to its homologue in Eriobotrya japonica (88%) and FaTFL1 showed the highest degree of similarity to its homologue in Fragaria vesca (99%). FaLFY expression mainly detected in the reproductive tissues, FaAP1 expression mainly detected in the reproductive tissues and partially in floral organs, and FaTLF1 expression mainly detected both in the reproductive tissues and young vegetative tissues. These results suggested that all these three genes may be involved in the flowering process of strawberry.

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Claims 8

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Partial sequences of three flowering related genes were isolated by PCR from strawberry Huaji.

Taxon
strawberryFragaria ×ananassae1
Material
Huajie1
Gene
FaLFY, FaAP1 and FaTFL1e1
Outcome
Partial sequencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Partial sequences of these three genes were isolated with PCR”

✓ located verbatim in the source · abstract, characters 317–378 · show in text ↑
e1

“three important flowering related genes (FaLFY, FaAP1 and FaTFL1) from cultivated strawberry 'Huaji'.”

✓ located verbatim in the source · abstract, characters 215–316 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaLFY amino acids had their highest homologue similarity to Rosa lucieae at 93%.

Taxon
STRAWBERRYFragaria ×ananassat0
Gene
FaLFYe0
Outcome
degree of similaritye0
Direction
higheste0
Value
93%e0
Comparator
Rosa lucieaee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The deduced amino acids of FaLFY showed the highest degree of similarity to its homologue in Rosa lucieae (93%)”

✓ located verbatim in the source · abstract, characters 467–578 · show in text ↑
t0

“CULTIVATED STRAWBERRY”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaAP1 had its highest homologue similarity to Eriobotrya japonica at 88%.

Taxon
STRAWBERRYFragaria ×ananassat0
Gene
FaAP1e0
Outcome
degree of similaritye0
Direction
higheste0
Value
88%e0
Comparator
Eriobotrya japonicae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“FaAP1 showed the highest degree of similarity to its homologue in Eriobotrya japonica (88%)”

✓ located verbatim in the source · abstract, characters 580–671 · show in text ↑
t0

“CULTIVATED STRAWBERRY”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaTFL1 had its highest homologue similarity to Fragaria vesca at 99%.

Taxon
STRAWBERRYFragaria ×ananassat0
Gene
FaTFL1e0
Outcome
degree of similaritye0
Direction
higheste0
Value
99%e0
Comparator
Fragaria vescae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“FaTFL1 showed the highest degree of similarity to its homologue in Fragaria vesca (99%).”

✓ located verbatim in the source · abstract, characters 676–764 · show in text ↑
t0

“CULTIVATED STRAWBERRY”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaLFY expression was mainly detected in reproductive tissues.

Taxon
STRAWBERRYFragaria ×ananassat0
Organ / stage
reproductive tissuese0
Gene
FaLFYe0
Outcome
expression mainly detectede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“FaLFY expression mainly detected in the reproductive tissues”

✓ located verbatim in the source · abstract, characters 765–825 · show in text ↑
t0

“CULTIVATED STRAWBERRY”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaAP1 expression was mainly detected in reproductive tissues and partially in floral organs.

Taxon
STRAWBERRYFragaria ×ananassat0
Organ / stage
reproductive tissuese0
Gene
FaAP1e0
Outcome
expression mainly detectede0
Qualifier
partially in floral organse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“FaAP1 expression mainly detected in the reproductive tissues and partially in floral organs”

✓ located verbatim in the source · abstract, characters 827–918 · show in text ↑
t0

“CULTIVATED STRAWBERRY”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaTLF1 expression was mainly detected in reproductive and young vegetative tissues.

Taxon
STRAWBERRYFragaria ×ananassat0
Organ / stage
reproductive tissues and young vegetative tissuese0
Gene
FaTLF1e0
Outcome
expression mainly detectede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“FaTLF1 expression mainly detected both in the reproductive tissues and young vegetative tissues.”

✓ located verbatim in the source · abstract, characters 924–1020 · show in text ↑
t0

“CULTIVATED STRAWBERRY”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Mechanismhypothesisoriginal resultbasis n/a

Claim · model-written summary

The authors suggest all three genes may participate in strawberry flowering.

Taxon
strawberryFragaria ×ananassae0
Gene
FaLFY, FaAP1 and FaTFL1e1
Outcome
flowering processe0
Qualifier
may be involvede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“These results suggested that all these three genes may be involved in the flowering process of strawberry.”

✓ located verbatim in the source · abstract, characters 1021–1127 · show in text ↑
e1

“three important flowering related genes (FaLFY, FaAP1 and FaTFL1)”

✓ located verbatim in the source · abstract, characters 215–280 · show in text ↑