Abstract
Petunia (Petunia sp.) and pink dianthus (Dianthus sp.) are important ornamental plants which are grown on balconies, in flower gardens, parks and other during summer period. Approximately 156 ornamental species are cultivated via tissue culture in different commercial laboratories worldwide. In vitro plant culture is one of the key tools in plant biotechnology that utilizes the totipotency of plant cells. In this research the influence of different concentrations and combinations of BA, GA3, IAA и NAA on meristem buds and cotyledons of petunia and pink dianthus as starting explants was studied. During the research development of starting explants was observed and certain changes were noted. The meristem buds cultivated on MS medium supplemented with certain growth regulators responded with development of callus firstly, and then leaf rosette and shoots. On the other hand, the meristem buds of pink dianthus developed merely shoots. All combinations of growth regulators under this study resulted in development of roots from shoots in pink dianthus, while in petunia, only the medium MS + 2 mg/l BA + 0,1 mg/l NAA stimulated root development in already formed shoots. The newly regenerants were acclimatized with their transfer to sterile mix of perlite and peat (1:1) in high moisture conditions. These research is base for further research on improvement of regeneration of different starting explants not only of petunia and dianthus, but also in other ornamental species.
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Claims 4
Claim · model-written summary
Petunia meristem buds on MS medium with growth regulators first formed callus, then leaf rosettes and shoots.
- Organ / stage
- meristem budse1
- Treatment
- MS medium supplemented with certain growth regulatorse1
- Outcome
- development of callus firstly, and then leaf rosette and shootse1
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PetuniaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“meristem buds and cotyledons of petunia and pink dianthus as starting explants”
e1“The meristem buds cultivated on MS medium supplemented with certain growth regulators responded with development of callus firstly, and then leaf rosette and shoots”
Claim · model-written summary
Pink dianthus meristem buds developed merely shoots.
Verbatim
e0“meristem buds and cotyledons of petunia and pink dianthus as starting explants”
e2“the meristem buds of pink dianthus developed merely shoots”
Claim · model-written summary
All growth regulator combinations resulted in root development from shoots in pink dianthus.
- Taxon
- pink dianthuse3
- Treatment
- All combinations of growth regulatorse3
- Outcome
- development of roots from shootse3
- Direction
- resulted ine3
Not reportedComparator
Verbatim
e3“All combinations of growth regulators under this study resulted in development of roots from shoots in pink dianthus”
Claim · model-written summary
In petunia, only MS + 2 mg/l BA + 0,1 mg/l NAA stimulated root development in already formed shoots.
- Treatment
- MS + 2 mg/l BA + 0,1 mg/l NAAe4
- Outcome
- root development in already formed shootse4
- Direction
- stimulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“in petunia, only the medium MS + 2 mg/l BA + 0,1 mg/l NAA stimulated root development in already formed shoots”
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- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.