Abstract
Petunia (Petunia hybrida) is currently one of the most favored ornamental potted flowers. Tissue culture-based propagation has proven to be effective in terms of multiplication rates and consistent seedling quality, meeting the demand for Purple Petunia varieties. In this study, Sodium hypochlorite (NaOCl) with various culture media [Murashige and Skoog (MS), ½ MS, and Knudson C] and growth regulators [6-benzyladenine (BA), Indole 3-butyric acid (IBA), and Naphthaleneacetic acid (NAA)] were utilized to determine the appropriate concentrations and durations for sample sterilization, shoot multiplication, and root induction processes of Purple Petunia. Evaluation criteria for in vitro Purple Petunia included parameters, such as survival rate, contamination rate, dead samples, number of shoots, shoot height, number of roots, and root length. The results indicated that stem sample sterilization at a 5% NaOCl concentration for 10 min yielded the highest survival rate (70.7%) at 14 days post-culture initiation. Purple Petunia stem samples, when cultured in MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBA, demonstrated the most efficient shoot multiplication with a multiplication rate of 26.9, a shoot weight of 3.6 g, a leaf/shoot ratio of 4.6, and a shoot height of 3.0 cm. The MS medium supplemented with 0.1 mg/L NAA was found to be optimal for root induction, resulting in the highest number of roots at 32.1 roots/plant and a root length of 7.0 cm. Briefly, this research provided fundamental insights into the in vitro propagation process of disease-free Purple Petunia multiplication.
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Claims 7
Claim · model-written summary
Sterilizing Petunia stems with 5% NaOCl for 10 min gave the highest survival rate of 70.7% at day 14.
- Organ / stage
- steme0
- Treatment
- 5% NaOCl concentration for 10 mine0
- Outcome
- survival ratee0
- Direction
- higheste0
- Value
- 70.7%e0
- Qualifier
- 14 days post-culture initiatione0
_flowers_in_Westgate_Gardens%2C_Canterbury.jpg?width=60){kind=small}
PetuniaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_flowers_in_Westgate_Gardens%2C_Canterbury.jpg?width=500)
Verbatim
e0“stem sample sterilization at a 5% NaOCl concentration for 10 min yielded the highest survival rate (70.7%) at 14 days post-culture initiation”
t0“Petunia (Petunia hybrida)”
Claim · model-written summary
MS medium with 0.5 mg/L BA and 0.1 mg/L IBA gave a shoot multiplication rate of 26.9.
- Organ / stage
- steme0
- Treatment
- MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBAe0
- Outcome
- multiplication ratee0
- Direction
- most efficiente0
- Value
- 26.9e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Purple Petunia stem samples, when cultured in MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBA, demonstrated the most efficient shoot multiplication with a multiplication rate of 26.9”
t0“Petunia (Petunia hybrida)”
Claim · model-written summary
The same medium gave shoot weight of 3.6 g.
- Treatment
- MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBAe1
- Outcome
- shoot weighte0
- Value
- 3.6 ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a shoot weight of 3.6 g”
e1“MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBA”
t0“Petunia (Petunia hybrida)”
Claim · model-written summary
The same medium gave a leaf/shoot ratio of 4.6.
- Treatment
- MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBAe1
- Outcome
- leaf/shoot ratioe0
- Value
- 4.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a leaf/shoot ratio of 4.6”
e1“MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBA”
t0“Petunia (Petunia hybrida)”
Claim · model-written summary
The same medium gave shoot height of 3.0 cm.
- Treatment
- MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBAe1
- Outcome
- shoot heighte0
- Value
- 3.0 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a shoot height of 3.0 cm”
e1“MS medium supplemented with 0.5 mg/L BA combined with 0.1 mg/L IBA”
t0“Petunia (Petunia hybrida)”
Claim · model-written summary
MS medium with 0.1 mg/L NAA produced the highest root count, 32.1 roots/plant.
- Treatment
- MS medium supplemented with 0.1 mg/L NAAe0
- Outcome
- number of rootse0
- Direction
- higheste0
- Value
- 32.1 roots/plante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The MS medium supplemented with 0.1 mg/L NAA was found to be optimal for root induction, resulting in the highest number of roots at 32.1 roots/plant”
t0“Petunia (Petunia hybrida)”
Claim · model-written summary
MS medium with 0.1 mg/L NAA produced root length of 7.0 cm.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 0.1 mg/L NAA was found to be optimal for root induction, resulting in the highest number of roots at 32.1 roots/plant and a root length of 7.0 cm”
t0“Petunia (Petunia hybrida)”
The same questions in other papers
1-naphthaleneacetic acid · growth · Petunia (petunia)Results agree
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- positive
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in vitro · growth · Petunia (petunia)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- no effect
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.