Paper · 2017 · Asian Journal of Pharmaceutical and Clinical Research

HPLC METHOD FOR QUANTIFICATION OF ELLAGIC ACID IN IN VIVO AND IN VITRO PLANT PARTS OF OROXYLUM INDICUM (L.) VENT.

Esha Rami; Saurabh Rami; Illa Patel

4
claims
8
verbatim quotes
3
citations
3
research areas
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Open access · cc-by-nc

Abstract

ABSTRACT Objective: The present study was performed to investigate the comparative analysis of ellagic acid content in in vivo (stem, leaf and root) and in vitro (callus, in vitro developed root and shoot) samples of Oroxylum indicum (L.) Vent. an important medicinal plant.Methods: For in vitro culture, seedling explants were inoculated on MS (Murashige and Skoog’s, 1962) medium, supplemented with BAP and Kn alone and combination with IAA. Analytical method HPLC was developed for the quantification of ellagic acid in in vivo and in vitro samples.Result: BAP combination with IAA was best for shoot multiplication, BAP with 2,4-D were used for callus proliferation. HPLC analysis of these extracts revealed that the quantity of ellagic acid present in both the samples but maximum ellagic acid content was obtained in leaves among all plant parts.Conclusion: Hence, the present study showed the in vivo as well as in vitro samples contain ellagic acid. That can be used for its large scale production in future. Key-words: Oroxylum indicum, ellagic acid, HPLC

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Among the plant parts of Oroxylum indicum, ellagic acid content was highest in leaves.

Taxon
OROXYLUM INDICUMOroxylum indicumt0
Organ / stage
leavese0
Outcome
ellagic acid contente0
Direction
maximume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oroxylum indicum
Oroxylum indicum · TaxonVinayaraj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“OROXYLUM INDICUM”

✓ located verbatim in the source · title, characters 86–102 · show in text ↑
e0

“maximum ellagic acid content was obtained in leaves among all plant parts”

✓ located verbatim in the source · abstract, characters 778–851 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Both in vivo and in vitro samples of Oroxylum indicum contain ellagic acid.

Taxon
OROXYLUM INDICUMOroxylum indicumt0
Outcome
ellagic acide0
Setting
in vivo as well as in vitro samplese0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oroxylum indicum
Oroxylum indicum · TaxonVinayaraj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“OROXYLUM INDICUM”

✓ located verbatim in the source · title, characters 86–102 · show in text ↑
e0

“the in vivo as well as in vitro samples contain ellagic acid”

✓ located verbatim in the source · abstract, characters 896–956 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP combined with IAA was best for shoot multiplication in Oroxylum indicum in vitro culture.

Taxon
OROXYLUM INDICUMOroxylum indicumt0
Treatment
BAP combination with IAAe0
Outcome
shoot multiplicatione0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oroxylum indicum
Oroxylum indicum · TaxonVinayaraj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“OROXYLUM INDICUM”

✓ located verbatim in the source · title, characters 86–102 · show in text ↑
e0

“BAP combination with IAA was best for shoot multiplication”

✓ located verbatim in the source · abstract, characters 560–618 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP with 2,4-D was used for callus proliferation in Oroxylum indicum in vitro culture.

Taxon
OROXYLUM INDICUMOroxylum indicumt0
Treatment
BAP with 2,4-De0
Outcome
callus proliferatione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oroxylum indicum
Oroxylum indicum · TaxonVinayaraj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“OROXYLUM INDICUM”

✓ located verbatim in the source · title, characters 86–102 · show in text ↑
e0

“BAP with 2,4-D were used for callus proliferation”

✓ located verbatim in the source · abstract, characters 620–669 · show in text ↑

2,4-D · growth · Oroxylum indicum (Indian trumpet tree)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

N-benzyladenine · growth · Oroxylum indicum (Indian trumpet tree)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.