Abstract
RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5' and 3' RNA fragments. The 5' cleaved RNA fragments are marked with uridylation for degradation. Here, we identified novel cofactors of Arabidopsis AGOs, named RICE1 and RICE2. RICE proteins specifically degraded single-strand (ss) RNAs in vitro; but neither miRNAs nor miRNA*s in vivo. RICE1 exhibited a DnaQ-like exonuclease fold and formed a homohexamer with the active sites located at the interfaces between RICE1 subunits. Notably, ectopic expression of catalytically-inactive RICE1 not only significantly reduced miRNA levels; but also increased 5' cleavage RISC fragments with extended uridine tails. We conclude that RICEs act to degrade uridylated 5' products of AGO cleavage to maintain functional RISC. Our study also suggests a possible link between decay of cleaved target mRNAs and miRNA stability in RISC.
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Claims 8
Claim · model-written summary
RICE1 and RICE2 were identified as Arabidopsis AGO cofactors.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- RICE1 and RICE2e0
- Outcome
- novel cofactorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified novel cofactors of Arabidopsis AGOs, named RICE1 and RICE2”
Claim · model-written summary
RICE proteins degraded single strand RNA in vitro.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RICE proteinse0
- Outcome
- degraded single-strand (ss) RNAse0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RICE proteins specifically degraded single-strand (ss) RNAs in vitro”
t0“Arabidopsis thaliana”
Claim · model-written summary
RICE proteins did not degrade miRNAs or miRNA*s in vivo.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RICE proteinse0
- Outcome
- neither miRNAs nor miRNA*s in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RICE proteins specifically degraded single-strand (ss) RNAs in vitro; but neither miRNAs nor miRNA*s in vivo”
t0“Arabidopsis thaliana”
Claim · model-written summary
RICE1 has a DnaQ like exonuclease fold and forms a homohexamer.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RICE1e0
- Outcome
- DnaQ-like exonuclease fold and formed a homohexamere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RICE1 exhibited a DnaQ-like exonuclease fold and formed a homohexamer with the active sites located at the interfaces between RICE1 subunits”
t0“Arabidopsis thaliana”
Claim · model-written summary
Catalytically inactive RICE1 expression reduced miRNA levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- ectopic expression of catalytically-inactive RICE1e0
- Gene
- RICE1e0
- Outcome
- miRNA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of catalytically-inactive RICE1 not only significantly reduced miRNA levels”
t0“Arabidopsis thaliana”
Claim · model-written summary
Catalytically inactive RICE1 expression increased uridylated cleavage fragments.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- ectopic expression of catalytically-inactive RICE1e0
- Gene
- RICE1e0
- Outcome
- 5' cleavage RISC fragments with extended uridine tailse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of catalytically-inactive RICE1 not only significantly reduced miRNA levels; but also increased 5' cleavage RISC fragments with extended uridine tails”
t0“Arabidopsis thaliana”
Claim · model-written summary
RICEs are proposed to degrade uridylated AGO cleavage products and maintain functional RISC.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RICEse0
- Outcome
- maintain functional RISCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RICEs act to degrade uridylated 5' products of AGO cleavage to maintain functional RISC”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors propose a link between cleavage product decay and miRNA stability.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RISCe0
- Outcome
- miRNA stability in RISCe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“possible link between decay of cleaved target mRNAs and miRNA stability in RISC”
t0“Arabidopsis thaliana”
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