Abstract
Lignification of cell wall appositions is a conserved basal defense mechanism in the plant innate immune response. However, the genetic pathway controlling defense-induced lignification remains unknown. Here, we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity. Loss of MYB15 reduces the content but not the composition of defense-induced lignin, whereas constitutive expression of MYB15 increases lignin content independently of immune activation. Comparative transcriptional and metabolomics analyses implicate MYB15 as necessary for the defense-induced synthesis of guaiacyl lignin and the basal synthesis of the coumarin metabolite scopoletin. MYB15 directly binds to the secondary wall MYB-responsive element consensus sequence, which encompasses the AC elements, to drive lignification. The myb15 and lignin biosynthetic mutants show increased susceptibility to the bacterial pathogen Pseudomonas syringae, consistent with defense-induced lignin having a major role in basal immunity. A scopoletin biosynthetic mutant also shows increased susceptibility independently of immune activation, consistent with a role in preformed defense. Our results support a role for phenylalanine-derived small molecules in preformed and inducible Arabidopsis defense, a role previously dominated by tryptophan-derived small molecules. Understanding the regulatory network linking lignin biosynthesis to plant growth and defense will help lignin engineering efforts to improve the production of biofuels and aromatic industrial products as well as increase disease resistance in energy and agricultural crops.
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Claims 9
Claim · model-written summary
Loss of MYB15 reduces the content of defense-induced lignin.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Loss of MYB15e1
- Gene
- MYB15e1
- Outcome
- defense-induced lignine1
- Direction
- reducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e1“Loss of MYB15 reduces the content but not the composition of defense-induced lignin”
Claim · model-written summary
Loss of MYB15 does not change the composition of defense-induced lignin.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of MYB15e1
- Gene
- MYB15e1
- Outcome
- composition of defense-induced lignine1
- Direction
- no_changeinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e1“Loss of MYB15 reduces the content but not the composition of defense-induced lignin”
Claim · model-written summary
Constitutive expression of MYB15 increases lignin content independently of immune activation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- constitutive expression of MYB15e2
- Gene
- MYB15e2
- Outcome
- lignin contente2
- Direction
- increasese2
- Qualifier
- independently of immune activatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e2“constitutive expression of MYB15 increases lignin content independently of immune activation”
Claim · model-written summary
MYB15 is implicated as necessary for defense-induced synthesis of guaiacyl lignin.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- MYB15e3
- Outcome
- defense-induced synthesis of guaiacyl lignine3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e3“implicate MYB15 as necessary for the defense-induced synthesis of guaiacyl lignin”
Claim · model-written summary
MYB15 is implicated in the basal synthesis of the coumarin metabolite scopoletin.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- MYB15e0
- Compound
- scopoletine4
- Outcome
- basal synthesis of the coumarin metabolite scopoletine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e4“the basal synthesis of the coumarin metabolite scopoletin”
Claim · model-written summary
MYB15 directly binds the secondary wall MYB-responsive element consensus sequence.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- MYB15e5
- Outcome
- binds to the secondary wall MYB-responsive element consensus sequencee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e5“MYB15 directly binds to the secondary wall MYB-responsive element consensus sequence, which encompasses the AC elements, to drive lignification”
Claim · model-written summary
MYB15 binding drives lignification.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- MYB15e5
- Outcome
- lignificatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e5“MYB15 directly binds to the secondary wall MYB-responsive element consensus sequence, which encompasses the AC elements, to drive lignification”
Claim · model-written summary
The myb15 and lignin biosynthetic mutants show increased susceptibility to Pseudomonas syringae.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- myb15 and lignin biosynthetic mutantse7
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee7pathogen - Outcome
- susceptibility to the bacterial pathogen Pseudomonas syringaee7
- Direction
- increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e7“The myb15 and lignin biosynthetic mutants show increased susceptibility to the bacterial pathogen Pseudomonas syringae”
Claim · model-written summary
A scopoletin biosynthetic mutant shows increased susceptibility independently of immune activation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- A scopoletin biosynthetic mutante1
- Compound
- scopoletine1
- Outcome
- susceptibilitye1
- Direction
- increasede1
- Qualifier
- independently of immune activatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate the Arabidopsis thaliana SG2-type R2R3-MYB transcription factor MYB15 as a regulator of defense-induced lignification and basal immunity”
e1“A scopoletin biosynthetic mutant also shows increased susceptibility independently of immune activation”
The same questions in other papers
overexpression · molecular: metabolites · Arabidopsis thaliana (thale cress)Positive and negative results
6 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- no effect
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.