Abstract
Dimocarpus longan Lour. is an evergreen fruit tree species of Sapindaceae family grown in subtropical region. Short flowering period coupled with narrow genetic base are the major constraints in longan genetic improvement. Pollens are known to directly influence reproductive success and genetic structure of the plant population. In this study, we compared the pollen quantity, viability and in vitro pollen germination of M1 and M2 type flowers of nine longan genotypes. Acetocarmine, 2, 3, 5-triphenyl tetrazolium chloride, 2, 5-diphenyl monotetrazolium bromide and aniline blue-lectophenol staining methods were used for pollen viability assessment while different concentrations of sucrose, boric acid (H3BO3) and agar were used for in vitro pollen germination test through hanging drop technique. Pollen tube growth was evaluated by incubating the media containing pollen at different temperature and duration. Highest pollen quantity per anther (5278) was observed in M2 flower of Lgc-06. Acetocarmine (2.0 %) showed best results for pollen viability (94.37 %) in M2 pollen of Lgc-06. Germination media containing 15 % sucrose + 100 ppm Boric acid + 0.5 % agar showed promising results for pollen germination (80.33 %). Highest germination rate (83.66 %) and maximum pollen tube growth was observed at 30oC for 24 hours. Overall, pollen from M2 flower had significantly higher pollen quantity, viability and in vitro pollen germination percentage compared to pollen from M1 flower.
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Claims 6
Claim · model-written summary
M2 flowers of Lgc-06 had the highest pollen quantity per anther, 5278.
- Taxon
- Longan
Dimocarpus longant0 - Material
- Lgc-06e0
- Organ / stage
- M2 flowere0
- Outcome
- pollen quantity per anthere0
- Direction
- Higheste0
- Value
- 5278e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest pollen quantity per anther (5278) was observed in M2 flower of Lgc-06.”
t0“Longan (Dimocarpus longan Lour.)”
Claim · model-written summary
Acetocarmine at 2.0 % gave the best pollen viability result, 94.37 %, in M2 pollen of Lgc-06.
- Taxon
- LonganDimocarpus longant0
- Material
- Lgc-06e0
- Organ / stage
- M2 pollene0
- Treatment
- Acetocarmine (2.0 %)e0
- Outcome
- pollen viabilitye0
- Direction
- beste0
- Value
- 94.37 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acetocarmine (2.0 %) showed best results for pollen viability (94.37 %) in M2 pollen of Lgc-06.”
t0“Longan (Dimocarpus longan Lour.)”
Claim · model-written summary
The medium with 15 % sucrose, 100 ppm boric acid and 0.5 % agar produced 80.33 % pollen germination.
- Taxon
- LonganDimocarpus longant0
- Treatment
- 15 % sucrose + 100 ppm Boric acid + 0.5 % agare0
- Outcome
- pollen germinatione0
- Value
- 80.33 %e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Germination media containing 15 % sucrose + 100 ppm Boric acid + 0.5 % agar showed promising results for pollen germination (80.33 %).”
t0“Longan (Dimocarpus longan Lour.)”
Claim · model-written summary
At 30oC for 24 hours pollen germination reached the highest rate, 83.66 %.
- Taxon
- LonganDimocarpus longant0
- Outcome
- germination ratee0
- Direction
- Higheste0
- Value
- 83.66 %e0
- Qualifier
- at 30oC for 24 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest germination rate (83.66 %) and maximum pollen tube growth was observed at 30oC for 24 hours.”
t0“Longan (Dimocarpus longan Lour.)”
Claim · model-written summary
Pollen tube growth was maximal at 30oC for 24 hours.
- Taxon
- LonganDimocarpus longant0
- Outcome
- pollen tube growthe0
- Direction
- maximume0
- Qualifier
- at 30oC for 24 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest germination rate (83.66 %) and maximum pollen tube growth was observed at 30oC for 24 hours.”
t0“Longan (Dimocarpus longan Lour.)”
Claim · model-written summary
M2 flowers had significantly higher pollen quantity, viability and in vitro germination than M1 flowers.
- Taxon
- LonganDimocarpus longant0
- Organ / stage
- M2 flowere0
- Outcome
- pollen quantity, viability and in vitro pollen germination percentagee0
- Direction
- highere0
- Comparator
- M1 flowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pollen from M2 flower had significantly higher pollen quantity, viability and in vitro pollen germination percentage compared to pollen from M1 flower.”
t0“Longan (Dimocarpus longan Lour.)”
The same questions in other papers
boric acid · yield · Dimocarpus longan (longan)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.