Abstract
A cDNA library was produced using mRNA extracted from ethylene-treated leaflet abscission zones of common elder (Sambucus nigra). Screening of the library with the insert from pBAC10, which encodes an abscission -1,4-glucanase (cellulase) from bean (Phaseolus vulgaris), resulted in the isolation of a near-full-length cDNA which was designated JET 1. Northern analysis, using JET 1 as a probe, detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation. Accumulation of the JET 1 transcript is promoted by ethylene and primarily restricted to the tissue comprising the abscission zone. Sequence analysis of JET 1 indicates it is 1768 bp in length and shares significant homology at the amino acid level with -1,4-glucanases from the leaf abscission zone of P. vulgaris (67%) and ripening avocado fruit (48%). The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites. Genomic Southern analysis of S. nigra DNA reveals that JET 1 may belong to a multi-gene family.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Screening isolated a near-full-length cDNA designated JET 1.
- Taxon
- Sambucus nigra
.jpg?width=60)
Sambucus nigrat0 - Gene
- JET 1e0
- Outcome
- isolation of a near-full-length cDNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“resulted in the isolation of a near-full-length cDNA which was designated JET 1”
t0“Sambucus nigra”
Claim · model-written summary
A 1.9 kb JET 1 transcript accumulated before visible cell separation.
- Taxon
- Sambucus nigraSambucus nigrat0
- Gene
- JET 1e1
- Outcome
- transcript of 1.9 kb that accumulated prior to the first visible signs of cell separatione0
- Value
- 1.9 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation”
e1“using JET 1 as a probe”
t0“Sambucus nigra”
Claim · model-written summary
Ethylene promoted JET 1 transcript accumulation.
- Taxon
- Sambucus nigraSambucus nigrat0
- Treatment
- ethylenee0
- Gene
- JET 1e0
- Outcome
- Accumulation of the JET 1 transcripte0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of the JET 1 transcript is promoted by ethylene”
t0“Sambucus nigra”
Claim · model-written summary
JET 1 transcript accumulation was concentrated in abscission-zone tissue.
- Taxon
- Sambucus nigraSambucus nigrat0
- Organ / stage
- tissue comprising the abscission zonee0
- Gene
- JET 1e1
- Outcome
- primarily restricted to the tissue comprising the abscission zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“primarily restricted to the tissue comprising the abscission zone”
e1“Accumulation of the JET 1 transcript”
t0“Sambucus nigra”
Claim · model-written summary
JET 1 sequence length was 1768 bp.
- Taxon
- Sambucus nigraSambucus nigrat0
- Gene
- JET 1e0
- Outcome
- lengthe0
- Value
- 1768 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JET 1 indicates it is 1768 bp in length”
t0“Sambucus nigra”
Claim · model-written summary
JET 1 had 67% amino-acid homology with bean leaf-abscission glucanases.
- Taxon
- Sambucus nigraSambucus nigrat0
- Gene
- JET 1e1
- Outcome
- homology at the amino acid levele0
- Value
- 67%e0
- Comparator
- -1,4-glucanases from the leaf abscission zone of P. vulgarise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shares significant homology at the amino acid level with -1,4-glucanases from the leaf abscission zone of P. vulgaris (67%)”
e1“Sequence analysis of JET 1 indicates it is 1768 bp in length”
t0“Sambucus nigra”
Claim · model-written summary
JET 1 had 48% amino-acid homology with ripening avocado-fruit glucanases.
- Taxon
- Sambucus nigraSambucus nigrat0
- Gene
- JET 1e2
- Outcome
- homology at the amino acid levele1
- Value
- 48%e0
- Comparator
- ripening avocado fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ripening avocado fruit (48%)”
e1“shares significant homology at the amino acid level with -1,4-glucanases”
e2“Sequence analysis of JET 1 indicates it is 1768 bp in length”
t0“Sambucus nigra”
Claim · model-written summary
The predicted elder enzyme peptide has two potential glycosylation sites.
- Taxon
- Sambucus nigraSambucus nigrat0
- Outcome
- potential glycosylation sitese0
- Value
- twoe0
- Qualifier
- predictede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites.”
t0“Sambucus nigra”
Claim · model-written summary
Genomic analysis suggests JET 1 may be part of a multi-gene family.
- Taxon
- Sambucus nigraSambucus nigrat0
- Gene
- JET 1e0
- Outcome
- may belong to a multi-gene familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JET 1 may belong to a multi-gene family”
t0“Sambucus nigra”
The same questions in other papers
ethene · molecular: expression · Sambucus nigra (European elder)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.