Abstract
Summary In vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40 min at room temperature with the PVS3 solution (glycerol 50% and sucrose 50%). They were subsequently frozen in individual microdroplets of vitrification solution, by direct immersion in liquid nitrogen (LN), and kept therein for 2, 4, 8 and 24 h. The explant rewarming was performed in an unloading solution (0.8 M sucrose) for 30 min at room temperature. The duration of LN exposure did not exert significant effects on the survival and regrowth of explants in both types of vitrification solutions. The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively. However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants. Monitoring the shoots regenerated in the in vitro culture revealed their normal capacity for multiplication and rooting in comparison with the controls, which fully confirms the purpose of cryopreservation in the long-term preservation of plant material.
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Claims 6
Claim · model-written summary
Duration of liquid nitrogen exposure did not significantly affect survival and regrowth of blackberry explants.
- Material
- ‘Čačanska Bestrna’e1
- Treatment
- duration of LN exposuree2
- Outcome
- survival and regrowth of explantse2
- Direction
- did not exert significant effectse2
RubusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“shoot tips of the blackberry cultivar ‘Čačanska Bestrna’”
e2“The duration of LN exposure did not exert significant effects on the survival and regrowth of explants in both types of vitrification solutions”
t0“Regrowth of Blackberry”
Claim · model-written summary
Survival of cryopreserved blackberry shoot tips dehydrated with PVS3 solution was 90–95%.
- Organ / stage
- shoot tipse3
- Treatment
- dehydrated with PVS3 solutione3
- Outcome
- survivale3
- Direction
- ranged betweene3
- Value
- 90–95%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively”
t0“Regrowth of Blackberry”
Claim · model-written summary
Regrowth of cryopreserved blackberry shoot tips dehydrated with PVS3 solution was 80–90%.
- Organ / stage
- shoot tipse3
- Treatment
- dehydrated with PVS3 solutione3
- Outcome
- regrowthe3
- Direction
- ranged betweene3
- Value
- 80–90%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively”
t0“Regrowth of Blackberry”
Claim · model-written summary
Dehydration with PVS A3 lowered survival of blackberry explants to 80–90%, compared with PVS3.
- Organ / stage
- explantse4
- Treatment
- dehydration with PVS A3e4
- Outcome
- survival ratee4
- Direction
- resulted in a lowere4
- Value
- 80–90%e4
- Comparator
- PVS3 solutione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants”
e3“The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively”
t0“Regrowth of Blackberry”
Claim · model-written summary
Dehydration with PVS A3 lowered regrowth of blackberry explants to 55–65%, compared with PVS3.
- Organ / stage
- explantse4
- Treatment
- dehydration with PVS A3e4
- Outcome
- regrowth ratee4
- Direction
- considerably lowere4
- Value
- 55–65%e4
- Comparator
- PVS3 solutione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants”
e3“The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively”
t0“Regrowth of Blackberry”
Claim · model-written summary
Regenerated blackberry shoots showed normal capacity for multiplication and rooting, similar to controls.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Monitoring the shoots regenerated in the in vitro culture revealed their normal capacity for multiplication and rooting in comparison with the controls”
t0“Regrowth of Blackberry”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.