Abstract
The droplet vitrification technique was adapted to blackberry 'Cacanska Bestrna' (Rubus fruticosus L.) by applying two different plant vitrification solutions as well as by optimizing the duration of vitrification treatments. Apical shoot tips were pretreated in liquid Murashige and Skoog (MS) medium with progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in solution containing 1.9 M glycerol and 0.5 M sucrose for 30 min. After loading, explants were dehydrated for 20, 30 and 40 min on ice with vitrification solution A3 (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40, 50 and 60 min at room temperature with PVS3 solution (glycerol 50% and sucrose 50%). Explants were frozen in individual microdroplets of vitrification solution by direct immersion in liquid nitrogen. After rapid rewarming performed by direct immersion in unloading solution containing 0.8 M sucrose for 30 min, explants were placed onto solid MS medium supplemented with 1 mg/L N-6-benzyladenine and 0.1 mg/L indole-3-butyric acid, cultivated in the dark for 7 days and than transferred to standard growth conditions. Under the described experimental conditions, survival and regrowth of cryopreserved shoot tips dehydrated on ice with A3 solution ranged between 45.0-90.9 and 25.0-61.8%, respectively. Longer exposure of explants to A3 solution brought about higher survival and regrowth rates. On the other hand, dehydration with PVS3 resulted in considerably higher survival rates (90.0-95.9%) and higher regrowth rates (77.3-90.0%) of cryopreserved explants. The shortest PVS3 treatment gave the highest survival and regrowth. The results obtained prove the feasibility of PVS3-based droplet vitrification technique for long-term storage of this genotype, while further research will focus on evaluation of the optimized protocol for its applicability to different genotypes of the Rubus genus.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
A3 dehydration yielded 45.0-90.9% survival in cryopreserved blackberry shoot tips.
RubusNot reportedMaterialDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“BLACKBERRY”
e0“survival and regrowth of cryopreserved shoot tips dehydrated on ice with A3 solution ranged between 45.0-90.9 and 25.0-61.8%, respectively”
Claim · model-written summary
A3 dehydration yielded 25.0-61.8% regrowth in cryopreserved blackberry shoot tips.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“BLACKBERRY”
e0“survival and regrowth of cryopreserved shoot tips dehydrated on ice with A3 solution ranged between 45.0-90.9 and 25.0-61.8%, respectively”
Claim · model-written summary
Longer A3 exposure increased survival and regrowth.
- Treatment
- Longer exposure of explants to A3 solutione0
- Outcome
- survival and regrowth ratese0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“BLACKBERRY”
e0“Longer exposure of explants to A3 solution brought about higher survival and regrowth rates.”
Claim · model-written summary
PVS3 dehydration yielded 90.0-95.9% survival, higher than A3 dehydration.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“BLACKBERRY”
e0“dehydration with PVS3 resulted in considerably higher survival rates (90.0-95.9%) and higher regrowth rates (77.3-90.0%) of cryopreserved explants”
Claim · model-written summary
PVS3 dehydration yielded 77.3-90.0% regrowth, higher than A3 dehydration.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“BLACKBERRY”
e0“dehydration with PVS3 resulted in considerably higher survival rates (90.0-95.9%) and higher regrowth rates (77.3-90.0%) of cryopreserved explants”
Claim · model-written summary
The shortest PVS3 treatment gave the highest survival and regrowth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“BLACKBERRY”
e0“The shortest PVS3 treatment gave the highest survival and regrowth.”
Claim · model-written summary
The PVS3 droplet vitrification technique was feasible for long-term storage of this genotype.
- Outcome
- feasibility of PVS3-based droplet vitrification technique for long-term storage of this genotypee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“BLACKBERRY”
e0“The results obtained prove the feasibility of PVS3-based droplet vitrification technique for long-term storage of this genotype”
The same questions in other papers
drought · survival · Rubus (bramble)Positive and negative results
1 in the opposite direction
- negative
drought · growth · Rubus (bramble)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
in vitro · growth · Rubus (bramble)Positive and negative results
12 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.