Abstract
Pumilio RNA-binding proteins are largely involved in mRNA degradation and translation repression. However, a few evolutionarily divergent Pumilios are also responsible for proper pre-rRNA processing in human and yeast. Here, we describe an essential Arabidopsis nucleolar Pumilio, APUM24, that is expressed in tissues undergoing rapid proliferation and cell division. A T-DNA insertion for APUM24 did not affect the male and female gametogenesis, but instead resulted in a negative female gametophytic effect on zygotic cell division immediately after fertilization. Additionally, the mutant embryos displayed defects in cell patterning from pro-embryo through globular stages. The mutant embryos were marked by altered auxin maxima, which were substantiated by the mislocalization of PIN1 and PIN7 transporters in the defective embryos. Homozygous apum24 callus accumulates rRNA processing intermediates, including uridylated and adenylated 5.8S and 25S rRNA precursors. An RNA-protein interaction assay showed that the histidine-tagged recombinant APUM24 binds RNAin vitro with no apparent specificity. Overall, our results demonstrated that APUM24 is required for rRNA processing and early embryogenesis in Arabidopsis.
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Claims 8
Claim · model-written summary
APUM24 is expressed in proliferating and dividing tissues.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- APUM24e0
- Outcome
- expressed in tissues undergoing rapid proliferation and cell divisione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“APUM24, that is expressed in tissues undergoing rapid proliferation and cell division.”
t0“Arabidopsis”
Claim · model-written summary
APUM24 insertion did not affect male and female gametogenesis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- A T-DNA insertion for APUM24e0
- Gene
- APUM24e0
- Outcome
- male and female gametogenesise0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A T-DNA insertion for APUM24 did not affect the male and female gametogenesis”
t0“Arabidopsis”
Claim · model-written summary
APUM24 insertion negatively affected zygotic cell division after fertilization through the female gametophyte.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- A T-DNA insertion for APUM24e1
- Outcome
- zygotic cell divisione0
- Direction
- negativee0
- Qualifier
- immediately after fertilizatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulted in a negative female gametophytic effect on zygotic cell division immediately after fertilization”
e1“A T-DNA insertion for APUM24”
t0“Arabidopsis”
Claim · model-written summary
Mutant embryos showed defective cell patterning from pro-embryo through globular stages.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- mutant embryose0
- Outcome
- defects in cell patterninge0
- Qualifier
- from pro-embryo through globular stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutant embryos displayed defects in cell patterning from pro-embryo through globular stages”
t0“Arabidopsis”
Claim · model-written summary
Mutant embryos had altered auxin maxima.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- mutant embryose0
- Outcome
- altered auxin maximae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutant embryos were marked by altered auxin maxima”
t0“Arabidopsis”
Claim · model-written summary
PIN1 and PIN7 transporters were mislocalized in defective embryos.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- defective embryose0
- Gene
- PIN1 and PIN7 transporterse0
- Outcome
- mislocalizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mislocalization of PIN1 and PIN7 transporters in the defective embryos”
t0“Arabidopsis”
Claim · model-written summary
Homozygous apum24 callus accumulated rRNA processing intermediates.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Homozygous apum24e0
- Organ / stage
- calluse0
- Outcome
- rRNA processing intermediatese0
- Qualifier
- including uridylated and adenylated 5.8S and 25S rRNA precursorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Homozygous apum24 callus accumulates rRNA processing intermediates, including uridylated and adenylated 5.8S and 25S rRNA precursors.”
t0“Arabidopsis”
Claim · model-written summary
Recombinant APUM24 bound RNA in vitro without apparent specificity.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- APUM24e0
- Outcome
- binds RNAin vitroe0
- Qualifier
- with no apparent specificitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“histidine-tagged recombinant APUM24 binds RNAin vitro with no apparent specificity”
t0“Arabidopsis”