Abstract
In northern China, low temperature is the most common abiotic stresses for tomato plants cultivated in solar‐greenhouse in winter. We recently found that the expression and enzyme activity of fructose‐1,6‐bisphosphate aldolases (FBAs) in tomato, which are important enzymes in the Calvin–Benson cycle (CBC), were significantly altered in tomato seedlings subjected to heat/cold stresses. In order to study the role of FBA in photosynthesis and in regulating cold stress responses of tomato seedlings ( Solanum lycopersicum ), we transformed a tomato inbred line (FF) with RNA interference (RNAi) vector containing SlFBA 7 reverse tandem repeat sequence. We found that the decreased SlFBA7 expression led to the decreased activities of FBA, as well as the activities of other main enzymes in the CBC. We also noticed a decrease in net photosynthetic rate, ribulose‐1,5‐bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild‐type. However, there are no changes in starch contents in the RNAi transgenic plants. RNAi SlFBA7 plants showed a decreased germination rate, and an increased levels of superoxide anions (O 2 ·‐ ) and hydrogen peroxide (H 2 O 2 ) under low temperature (8/5°C) and low‐light intensity (100 μmol m −2 s −1 photon flux density) growth conditions. These findings demonstrated the important role of SlFBA7 in regulating growth and chilling tolerance of tomato seedlings, and suggested that the catalytic activity of FBA in the CBC is sensitive to temperature.
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Claims 13
Claim · model-written summary
Reduced SlFBA7 expression decreased FBA activity.
- Taxon
- tomato seedlings
Solanum lycopersicumt0 - Treatment
- decreased SlFBA7 expressione0
- Gene
- SlFBA7e0
- Outcome
- activities of FBAe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“the decreased SlFBA7 expression led to the decreased activities of FBA, as well as the activities of other main enzymes in the CBC”
Claim · model-written summary
Reduced SlFBA7 expression decreased activities of other main Calvin cycle enzymes.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- decreased SlFBA7 expressione0
- Gene
- SlFBA7e0
- Outcome
- activities of other main enzymes in the CBCe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“the decreased SlFBA7 expression led to the decreased activities of FBA, as well as the activities of other main enzymes in the CBC”
Claim · model-written summary
RNAi SlFBA7 plants had decreased net photosynthetic rate relative to wild type.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- net photosynthetic ratee0
- Direction
- decreasee0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“a decrease in net photosynthetic rate, ribulose‐1,5‐bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild‐type”
Claim · model-written summary
RNAi SlFBA7 plants had decreased ribulose‐1,5‐bisphosphate relative to wild type.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- ribulose‐1,5‐bisphosphatee0
- Direction
- decreasee0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“a decrease in net photosynthetic rate, ribulose‐1,5‐bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild‐type”
Claim · model-written summary
RNAi SlFBA7 plants had decreased soluble sugar content relative to wild type.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- soluble sugar contente0
- Direction
- decreasee0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“a decrease in net photosynthetic rate, ribulose‐1,5‐bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild‐type”
Claim · model-written summary
RNAi SlFBA7 plants had decreased stem diameter relative to wild type.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- stem diametere0
- Direction
- decreasee0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“a decrease in net photosynthetic rate, ribulose‐1,5‐bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild‐type”
Claim · model-written summary
RNAi SlFBA7 plants had decreased dry weight relative to wild type.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- dry weighte0
- Direction
- decreasee0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“a decrease in net photosynthetic rate, ribulose‐1,5‐bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild‐type”
Claim · model-written summary
RNAi SlFBA7 plants had decreased seed size relative to wild type.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- seed sizee0
- Direction
- decreasee0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“a decrease in net photosynthetic rate, ribulose‐1,5‐bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild‐type”
Claim · model-written summary
RNAi SlFBA7 plants showed no change in starch content.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi transgenic plantse0
- Outcome
- starch contentse0
- Direction
- no changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“there are no changes in starch contents in the RNAi transgenic plants”
Claim · model-written summary
RNAi SlFBA7 plants showed a decreased germination rate under low temperature and low light.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- germination ratee0
- Direction
- decreasede0
- Qualifier
- low temperature (8/5°C) and low‐light intensity (100 μmol m −2 s −1 photon flux density)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“RNAi SlFBA7 plants showed a decreased germination rate, and an increased levels of superoxide anions (O 2 ·‐ ) and hydrogen peroxide (H 2 O 2 ) under low temperature (8/5°C) and low‐light intensity (100 μmol m −2 s −1 photon flux density) growth conditions”
Claim · model-written summary
RNAi SlFBA7 plants showed increased superoxide anions under low temperature and low light.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- superoxide anionse0
- Direction
- increasede0
- Qualifier
- low temperature (8/5°C) and low‐light intensity (100 μmol m −2 s −1 photon flux density)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“RNAi SlFBA7 plants showed a decreased germination rate, and an increased levels of superoxide anions (O 2 ·‐ ) and hydrogen peroxide (H 2 O 2 ) under low temperature (8/5°C) and low‐light intensity (100 μmol m −2 s −1 photon flux density) growth conditions”
Claim · model-written summary
RNAi SlFBA7 plants showed increased hydrogen peroxide under low temperature and low light.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Treatment
- RNAi SlFBA7 plantse0
- Outcome
- hydrogen peroxidee0
- Direction
- increasede0
- Qualifier
- low temperature (8/5°C) and low‐light intensity (100 μmol m −2 s −1 photon flux density)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“RNAi SlFBA7 plants showed a decreased germination rate, and an increased levels of superoxide anions (O 2 ·‐ ) and hydrogen peroxide (H 2 O 2 ) under low temperature (8/5°C) and low‐light intensity (100 μmol m −2 s −1 photon flux density) growth conditions”
Claim · model-written summary
The authors infer that FBA catalytic activity in the Calvin cycle is temperature sensitive.
- Taxon
- tomato seedlingsSolanum lycopersicumt0
- Gene
- FBAe0
- Outcome
- catalytic activity of FBA in the CBCe0
- Qualifier
- sensitive to temperaturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato seedlings”
e0“the catalytic activity of FBA in the CBC is sensitive to temperature”