Abstract
Sugarcane contributes 60–70% of annual sugar production in the world. Somaclonal variation has potential to enhance genetic variation present within a species. Present study was done to optimize an in vitro propagation protocol for sugarcane. The experiments included four varieties, 9 callus induction media, 27 regeneration media, and 9 root induction media under two-factor factorial CRD. Data were recorded on callus induction, embryogenic callus formation, shoot elongation (cm), root induction, and plant regeneration. Statistically significant differences existed between genotypes and treatments for callus induction (%), embryogenic callus formation (%), shoot elongation (cm), root induction, and plant regeneration (%). All parameters showed dependency on genotypes, culture media, and their interaction. Highest callus induction (95%) embryogenic callus formation (95%) was observed in callus induction media 5. Highest plantlet regeneration (98.9%) capacity was observed in regeneration media 11 whereas maximum shoot elongation (12.13 cm) and root induction (8.32) were observed in rooting media 4. G1 showed best response for all traits and vice versa for G4. Hence it was concluded that G1, callus induction media 5, regeneration media 11, and rooting media 4 are the best conditions for in vitro propagation of sugarcane.
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Claims 9
Claim · model-written summary
Genotypes and treatments differed significantly for callus, shoot, root, and regeneration traits.
Verbatim
e0“Statistically significant differences existed between genotypes and treatments for callus induction (%), embryogenic callus formation (%), shoot elongation (cm), root induction, and plant regeneration (%).”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
All measured parameters depended on genotypes, culture media and their interaction.
Not reportedDirection
Verbatim
e0“All parameters showed dependency on genotypes, culture media, and their interaction.”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
Callus induction media 5 yielded the highest callus induction of 95%.
- Taxon
- Sugarcanet0
- Treatment
- callus induction media 5e0
- Outcome
- callus inductione0
- Direction
- Higheste0
- Value
- 95%e0
Not reportedComparator
Verbatim
e0“Highest callus induction (95%) embryogenic callus formation (95%) was observed in callus induction media 5.”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
Callus induction media 5 yielded the highest embryogenic callus formation of 95%.
- Taxon
- Sugarcanet0
- Treatment
- callus induction media 5e0
- Outcome
- embryogenic callus formatione0
- Direction
- Higheste0
- Value
- 95%e0
Not reportedComparator
Verbatim
e0“Highest callus induction (95%) embryogenic callus formation (95%) was observed in callus induction media 5.”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
Regeneration media 11 had the highest plantlet regeneration capacity at 98.9%.
- Taxon
- Sugarcanet0
- Treatment
- regeneration media 11e0
- Outcome
- plantlet regeneratione0
- Direction
- Higheste0
- Value
- 98.9%e0
Not reportedComparator
Verbatim
e0“Highest plantlet regeneration (98.9%) capacity was observed in regeneration media 11”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
Rooting media 4 produced maximum shoot elongation of 12.13 cm.
- Taxon
- Sugarcanet0
- Treatment
- rooting media 4e0
- Outcome
- shoot elongatione0
- Direction
- maximume0
- Value
- 12.13 cme0
Not reportedComparator
Verbatim
e0“maximum shoot elongation (12.13 cm) and root induction (8.32) were observed in rooting media 4.”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
Rooting media 4 produced maximum root induction of 8.32.
Not reportedComparator
Verbatim
e0“maximum shoot elongation (12.13 cm) and root induction (8.32) were observed in rooting media 4.”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
G1 gave the best response across traits.
Verbatim
e0“G1 showed best response for all traits and vice versa for G4.”
t0“Sugarcane (Saccharum officinarum)”
Claim · model-written summary
The authors identified G1 and media 5, 11 and 4 as best conditions for propagation.
Verbatim
e0“Hence it was concluded that G1, callus induction media 5, regeneration media 11, and rooting media 4 are the best conditions for in vitro propagation of sugarcane.”
The same questions in other papers
in vitro · growth · Saccharum officinarum (sugarcane)Positive and negative results
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