Abstract
Background and Aims: Although several studies have confirmed the beneficial roles of exogenous melatonin in lateral root (LR) formation, the molecular mechanism is still elusive. Here, the role of hydrogen peroxide (H2O2) in the induction of LR formation triggered by melatonin was investigated. Methods: Alfalfa (Medicago sativa 'Biaogan') and transgenic Arabidopsis seedlings were treated with or without melatonin, diphenyleneiodonium (DPI, NADPH oxidase inhibitor), N,N'-dimethylthiourea (DMTU, H2O2 scavenger), alone or combined. Then, H2O2 content was determined with 2',7'-dichlorofluorescein diacetate (H2DCFDA)-dependent fluorescence and spectrophotography. Transcript levels of cell cycle regulatory genes were analysed by real-time reverse transcription-PCR. Key Results: Application of exogenous melatonin not only increased endogenous H2O2 content but also induced LR formation in alfalfa seedlings. Consistently, melatonin-induced LR primordia exhibited an accelerated response. These inducible responses were significantly blocked when DPI or DMTU was applied. Compared with the wild-type, transgenic Arabidopsis plants overexpressing alfalfa MsSNAT (a melatonin synthesis gene) increased H2O2 accumulation and thereafter LR formation, both of which were blocked by DPI or DMTU. Similarly, melatonin-modulated expression of marker genes responsible for LR formation, including MsCDKB1;1, MsCDKB2;1, AtCDKB1;1 and AtCDKB2;1, was obviously impaired by the removal of H2O2 in both alfalfa and transgenic Arabidopsis plants. Conclusions: Pharmacological and genetic evidence revealed that endogenous melatonin-triggered LR formation was H2O2-dependent.
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Claims 8
Claim · model-written summary
Exogenous melatonin increased endogenous H2O2 content in alfalfa seedlings relative to untreated seedlings.
- Taxon
- Medicago sativa
Medicago sativae0 - Treatment
- exogenous melatonine1
- Outcome
- endogenous H2O2 contente1
- Direction
- increasede1
- Comparator
- without melatonine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa 'Biaogan') and transgenic Arabidopsis seedlings were treated with or without melatonin”
e1“Application of exogenous melatonin not only increased endogenous H2O2 content but also induced LR formation in alfalfa seedlings”
Claim · model-written summary
Exogenous melatonin induced lateral root formation in alfalfa seedlings.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- exogenous melatonine1
- Outcome
- LR formatione1
- Direction
- inducede1
- Comparator
- without melatonine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa 'Biaogan') and transgenic Arabidopsis seedlings were treated with or without melatonin”
e1“Application of exogenous melatonin not only increased endogenous H2O2 content but also induced LR formation in alfalfa seedlings”
Claim · model-written summary
Melatonin-induced lateral root primordia showed an accelerated response.
- Taxon
- Medicago sativaMedicago sativae0
- Treatment
- melatonine2
- Outcome
- LR primordiae2
- Direction
- acceleratede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa 'Biaogan') and transgenic Arabidopsis seedlings were treated with or without melatonin”
e2“Consistently, melatonin-induced LR primordia exhibited an accelerated response”
Claim · model-written summary
The melatonin-induced responses were significantly blocked when DPI or DMTU was applied in alfalfa.
- Taxon
- Medicago sativaMedicago sativae0
- Treatment
- DPI or DMTUe3
- Outcome
- inducible responsese3
- Direction
- blockede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa 'Biaogan') and transgenic Arabidopsis seedlings were treated with or without melatonin”
e3“These inducible responses were significantly blocked when DPI or DMTU was applied”
Claim · model-written summary
Transgenic Arabidopsis overexpressing alfalfa MsSNAT increased H2O2 accumulation and lateral root formation compared with the wild-type.
- Taxon
- Arabidopsis
Arabidopsise4 - Material
- transgenic Arabidopsis plantse4
- Treatment
- overexpressing alfalfa MsSNATe4
- Gene
- MsSNATe4
- Outcome
- H2O2 accumulation and thereafter LR formatione4
- Direction
- increasede4
- Comparator
- wild-typee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Compared with the wild-type, transgenic Arabidopsis plants overexpressing alfalfa MsSNAT (a melatonin synthesis gene) increased H2O2 accumulation and thereafter LR formation”
Claim · model-written summary
The H2O2 accumulation and lateral root formation in transgenic Arabidopsis were both blocked by DPI or DMTU.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- DPI or DMTUe5
- Outcome
- H2O2 accumulation and thereafter LR formatione4
- Direction
- blockede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Compared with the wild-type, transgenic Arabidopsis plants overexpressing alfalfa MsSNAT (a melatonin synthesis gene) increased H2O2 accumulation and thereafter LR formation”
e5“both of which were blocked by DPI or DMTU”
Claim · model-written summary
Melatonin-modulated expression of LR marker genes was impaired by removing H2O2 in alfalfa and transgenic Arabidopsis.
- Taxon
- alfalfa and transgenic ArabidopsisArabidopsisMedicago sativae6
- Treatment
- the removal of H2O2e6
- Gene
- MsCDKB1;1, MsCDKB2;1, AtCDKB1;1 and AtCDKB2;1e6
- Outcome
- expression of marker genese6
- Direction
- impairede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“melatonin-modulated expression of marker genes responsible for LR formation, including MsCDKB1;1, MsCDKB2;1, AtCDKB1;1 and AtCDKB2;1, was obviously impaired by the removal of H2O2 in both alfalfa and transgenic Arabidopsis plants”
Claim · model-written summary
Melatonin-triggered lateral root formation was H2O2-dependent.
- Taxon
- Medicago sativaMedicago sativae0
- Compound
- H2O2e7
- Outcome
- endogenous melatonin-triggered LR formatione7
- Qualifier
- H2O2-dependente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa 'Biaogan') and transgenic Arabidopsis seedlings were treated with or without melatonin”
e7“Pharmacological and genetic evidence revealed that endogenous melatonin-triggered LR formation was H2O2-dependent”
The same questions in other papers
melatonin · growth · Medicago sativa (alfalfa)Results differ
13 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
11 papers more in this group
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