Abstract
The CRISPR-Cas9-based multiplexed gene editing (MGE) provides a powerful method to modify multiple genomic regions simultaneously controlling different agronomic traits in crops. We applied the MGE construct built by combining the tandemly arrayed tRNA–gRNA units to generate heritable mutations in the TaGW2, TaLpx-1, and TaMLO genes of hexaploid wheat. The knockout mutations generated by this construct in all three homoeologous copies of one of the target genes, TaGW2, resulted in a substantial increase in seed size and thousand grain weight. We showed that the non-modified gRNA targets in the early generation plants can be edited by CRISPR-Cas9 in the following generations. Our results demonstrate that transgenerational gene editing activity can serve as the source of novel variation in the progeny of CRISPR-Cas9-expressing plants and suggest that the Cas9-inducible trait transfer for crop improvement can be achieved by crossing the plants expressing the gene editing constructs with the lines of interest.
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Claims 5
Claim · model-written summary
Editing all three TaGW2 homoeologs increased wheat seed size.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- knockout mutationse0
- Gene
- TaGW2e0
- Outcome
- seed sizee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The knockout mutations generated by this construct in all three homoeologous copies of one of the target genes, TaGW2, resulted in a substantial increase in seed size and thousand grain weight”
t0“Wheat”
Claim · model-written summary
Editing all three TaGW2 homoeologs increased wheat thousand grain weight.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- knockout mutationse0
- Gene
- TaGW2e0
- Outcome
- thousand grain weighte0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The knockout mutations generated by this construct in all three homoeologous copies of one of the target genes, TaGW2, resulted in a substantial increase in seed size and thousand grain weight”
t0“Wheat”
Claim · model-written summary
CRISPR-Cas9 edited initially unmodified gRNA targets in later generations.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- edited by CRISPR-Cas9e0
- Qualifier
- following generationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the non-modified gRNA targets in the early generation plants can be edited by CRISPR-Cas9 in the following generations”
t0“Wheat”
Claim · model-written summary
Transgenerational editing can supply novel variation in progeny.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- novel variatione0
- Qualifier
- transgenerational gene editing activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenerational gene editing activity can serve as the source of novel variation in the progeny of CRISPR-Cas9-expressing plants”
t0“Wheat”
Claim · model-written summary
The authors suggest transferring Cas9-inducible traits by crossing construct-expressing plants with lines of interest.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- Cas9-inducible trait transfer for crop improvemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggest that the Cas9-inducible trait transfer for crop improvement can be achieved by crossing the plants expressing the gene editing constructs with the lines of interest”
t0“Wheat”
The same questions in other papers
loss of function · yield · Triticum aestivum (wheat)Positive and negative results
17 papers in the same direction as this paper · 25 in the opposite direction
- positive
- positive
- no effect
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.