Abstract
Enhancement of micronutrient bioavailability is crucial to address the malnutrition in the developing countries. Various approaches employed to address the micronutrient bioavailability are showing promising signs, especially in cereal crops. Phytic acid (PA) is considered as a major antinutrient due to its ability to chelate important micronutrients and thereby restricting their bioavailability. Therefore, manipulating PA biosynthesis pathway has largely been explored to overcome the negative impact in different crop species. Recently, we reported that functional wheat inositol pentakisphosphate kinase (TaIPK1) is involved in PA biosynthesis, however the functional roles of the IPK1 gene in wheat remains elusive. In this study, RNAi-mediated gene silencing was performed for IPK1 transcripts in hexaploid wheat. Four non-segregating RNAi lines of wheat were selected for detailed study (S3-D-6-1; S6-K-3-3; S6-K-6-10 and S16-D-9-5). Homozygous transgenic RNAi lines at T4 seeds with a decreased transcript of TaIPK1 showed 28%-56% reduction of the PA. Silencing of IPK1 also resulted in increased free phosphate in mature grains. Although, no phenotypic changes in the spike was observed but, lowering of grain PA resulted in the reduced number of seeds per spikelet. The lowering of grain PA was also accompanied by a significant increase in iron (Fe) and zinc (Zn) content, thereby enhancing their molar ratios (Zn:PA and Fe:PA). Overall, this work suggests that IPK1 is a promising candidate for employing genome editing tools to address the mineral accumulation in wheat grains.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
TaIPK1 RNAi lines had 28%-56% less grain phytic acid.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- RNAie0
- Gene
- TaIPK1e0
- Outcome
- PAe0
- Direction
- reductione0
- Value
- 28%-56%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Homozygous transgenic RNAi lines at T4 seeds with a decreased transcript of TaIPK1 showed 28%-56% reduction of the PA.”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
IPK1 silencing increased free phosphate in mature grains.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- mature grainse0
- Treatment
- Silencing of IPK1e0
- Gene
- IPK1e0
- Outcome
- free phosphatee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of IPK1 also resulted in increased free phosphate in mature grains.”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
IPK1 silencing did not visibly change spike phenotype.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- RNAi-Mediated Downregulationt0
- Outcome
- phenotypic changes in the spikee0
- Direction
- noe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no phenotypic changes in the spike was observed”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
Lower grain phytic acid was associated with fewer seeds per spikelet.
- Taxon
- wheatTriticum aestivumt0
- Compound
- PAe0
- Outcome
- number of seeds per spikelete0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lowering of grain PA resulted in the reduced number of seeds per spikelet”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
Lower grain phytic acid was accompanied by increased iron content.
- Taxon
- wheatTriticum aestivumt0
- Compound
- PAe0
- Outcome
- iron (Fe)e0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowering of grain PA was also accompanied by a significant increase in iron (Fe) and zinc (Zn) content, thereby enhancing their molar ratios (Zn:PA and Fe:PA).”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
Lower grain phytic acid was accompanied by increased zinc content.
- Taxon
- wheatTriticum aestivumt0
- Compound
- PAe0
- Outcome
- zinc (Zn) contente0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowering of grain PA was also accompanied by a significant increase in iron (Fe) and zinc (Zn) content, thereby enhancing their molar ratios (Zn:PA and Fe:PA).”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
The Zn:PA molar ratio increased as grain phytic acid fell.
- Taxon
- wheatTriticum aestivumt0
- Compound
- PAe0
- Outcome
- Zn:PAe0
- Direction
- enhancinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowering of grain PA was also accompanied by a significant increase in iron (Fe) and zinc (Zn) content, thereby enhancing their molar ratios (Zn:PA and Fe:PA).”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
The Fe:PA molar ratio increased as grain phytic acid fell.
- Taxon
- wheatTriticum aestivumt0
- Compound
- PAe0
- Outcome
- Fe:PAe0
- Direction
- enhancinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowering of grain PA was also accompanied by a significant increase in iron (Fe) and zinc (Zn) content, thereby enhancing their molar ratios (Zn:PA and Fe:PA).”
t0“RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation”
Claim · model-written summary
The authors propose IPK1 as a genome editing target for wheat grain mineral accumulation.
- Taxon
- wheatTriticum aestivume0
- Gene
- IPK1e0
- Outcome
- mineral accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IPK1 is a promising candidate for employing genome editing tools to address the mineral accumulation in wheat grains.”
The same questions in other papers
loss of function · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
13 papers in the same direction as this paper · 18 in the opposite direction
- negative
- no effect
- negative
28 papers more in this group
See all 32 papers →loss of function · yield · Triticum aestivum (wheat)Positive and negative results
24 papers in the same direction as this paper · 18 in the opposite direction
- positive
- positive
- positive
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.