Abstract
is an important synthetic auxin and it is one of the most widely used herbicides in agriculture. The use of picloram is representing a potential hazardous to ecosystems and the human health. Putrescine is a kind of polyamine which has a role in plant metabolism such as protecting membrane stability, removing free radicals and nucleic acid and protein synthesis, etc. In this study, DNA damage levels in Phaseolus vulgaris treated with picloram, DNA methylation changes and whether putrescine was any alleviative impact on these alterations were investigated. DNA methylation pattern changes and DNA damage levels were determined by using Randomly Amplified Polymorphic DNA (RAPDs) and Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RAs), respectively. The obtained results indicated that all doses of picloram (5, 10, 20 and 40 mg L -1 ) had a negative effect on RAPDs profile changes (increased DNA damage levels) and decreased of Genomic Template Stability (GTS) and also DNA hypomethylation was seen. However, all concentrations of putrescine (0.01, 0.1 and 1 ppm) reduced these harmful effects of picloram. Consequently, putrescine can be an alternative for reducing genotoxic damage against chemical mutagens in plants.
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Claims 5
Claim · model-written summary
All tested picloram doses increased DNA damage in Phaseolus vulgaris.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Treatment
- picloram (5, 10, 20 and 40 mg L -1 )e1
- Outcome
- DNA damage levelse0
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA damage levels in Phaseolus vulgaris treated with picloram”
e1“all doses of picloram (5, 10, 20 and 40 mg L -1 ) had a negative effect on RAPDs profile changes (increased DNA damage levels)”
Claim · model-written summary
All tested picloram doses decreased genomic template stability.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Treatment
- picloram (5, 10, 20 and 40 mg L -1 )e0
- Outcome
- Genomic Template Stability (GTS)e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all doses of picloram (5, 10, 20 and 40 mg L -1 ) had a negative effect on RAPDs profile changes (increased DNA damage levels) and decreased of Genomic Template Stability (GTS)”
e1“Phaseolus vulgaris treated with picloram”
Claim · model-written summary
Picloram treatment caused DNA hypomethylation.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Treatment
- piclorame1
- Outcome
- DNA hypomethylatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA hypomethylation was seen.”
e1“Phaseolus vulgaris treated with picloram”
Claim · model-written summary
All tested putrescine concentrations reduced the harmful effects of picloram.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Treatment
- putrescine (0.01, 0.1 and 1 ppm)e0
- Outcome
- harmful effects of piclorame0
- Direction
- reducede0
- Comparator
- piclorame0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all concentrations of putrescine (0.01, 0.1 and 1 ppm) reduced these harmful effects of picloram.”
e1“DNA damage levels in Phaseolus vulgaris treated with picloram”
Claim · model-written summary
The authors propose putrescine as an alternative for reducing genotoxic damage from chemical mutagens in plants.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Outcome
- alternative for reducing genotoxic damage against chemical mutagens in plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“putrescine can be an alternative for reducing genotoxic damage against chemical mutagens in plants.”
e1“Phaseolus vulgaris treated with picloram”
The same questions in other papers
putrescine · molecular: metabolites · Phaseolus vulgaris (common bean)Results differ
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.