Abstract
Water scarcity is a major abiotic stress factor that strongly influences growth, development and yield of grain legumes in arid and semi-arid area of the world. Drought stress frequently occurs during the seedling stage and finally affects yield of faba bean ( Vicia faba L.). However, the responses of plant leaf to drought have not been documented very well at the proteomic level. “Ga da dou” of the drought-tolerant faba bean cultivar was exposed to drought to examine the proteome changes of leaves. In this study, 2-week-old seedlings were subjected to water deficit by 7 days drought stress, whereas control plants were regularly irrigated. After withdrawing water, plants exposed to drought for 7 days and control plants at the same developmental stage were included in quantitative proteomic analysis using two-dimensional electrophoresis gels of proteins in combination with mass spectrometry. Over 300 proteins were detected by 2-DE, 50 differentially expressed proteins were detected by t test and 30 proteins were successfully identified by MALDI-TOF/TOF, in which 25 proteins were clearly downregulated and five proteins were upregulated. The quantified proteins were grouped into five functional groups, mainly regulatory proteins (46.7%), energy metabolism (23.3%), cell cytoskeleton (6.7%), other functions (20%) and unknown function (3.3%). Chitinase was upregulated under drought, suggesting that it was an important part of the plant defense system, playing an important role in stress resistance. 50S ribosomal protein was upregulated under drought, suggesting its role in protecting plants against stress by re-establishing normal protein conformations. The abundance of proteins involved in protein synthesis such as chitinase, Bet protein and glutamate–glyoxylate aminotransferase was upregulated under drought stress. These proteins could play important roles in drought tolerance and contribute to the relatively stronger drought tolerance of “Ga da dou”.
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Claims 14
Claim · model-written summary
Over 300 proteins were detected by 2-DE in faba bean leaves.
- Taxon
- Vicia faba L.
Vicia fabat0 - Outcome
- proteins were detected by 2-DEe0
- Value
- Over 300e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e0“Over 300 proteins were detected by 2-DE”
Claim · model-written summary
50 differentially expressed proteins were detected by t test.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- differentially expressed proteinse1
- Value
- 50e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e1“50 differentially expressed proteins were detected by t test”
Claim · model-written summary
30 proteins were successfully identified by MALDI-TOF/TOF.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- successfully identifiede2
- Value
- 30e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e2“30 proteins were successfully identified by MALDI-TOF/TOF”
Claim · model-written summary
Under drought, 25 proteins were downregulated.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- water deficit by 7 days drought stresse1
- Outcome
- proteinse3
- Direction
- downregulatede3
- Value
- 25e3
- Comparator
- control plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e3“25 proteins were clearly downregulated”
e1“seedlings were subjected to water deficit by 7 days drought stress”
e2“control plants were regularly irrigated”
Claim · model-written summary
Under drought, five proteins were upregulated.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- water deficit by 7 days drought stresse1
- Outcome
- proteinse4
- Direction
- upregulatede4
- Value
- fivee4
- Comparator
- control plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e4“five proteins were upregulated”
e1“seedlings were subjected to water deficit by 7 days drought stress”
e2“control plants were regularly irrigated”
Claim · model-written summary
Regulatory proteins made up 46.7% of the quantified proteins.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- regulatory proteinse6
- Value
- 46.7%e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e6“mainly regulatory proteins (46.7%)”
Claim · model-written summary
Energy metabolism proteins made up 23.3% of the quantified proteins.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- energy metabolisme7
- Value
- 23.3%e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e7“energy metabolism (23.3%)”
Claim · model-written summary
Cell cytoskeleton proteins made up 6.7% of the quantified proteins.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- cell cytoskeletone7
- Value
- 6.7%e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e7“cell cytoskeleton (6.7%), other functions (20%) and unknown function (3.3%)”
Claim · model-written summary
Other-function proteins made up 20% of the quantified proteins.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- other functionse7
- Value
- 20%e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e7“cell cytoskeleton (6.7%), other functions (20%) and unknown function (3.3%)”
Claim · model-written summary
Unknown-function proteins made up 3.3% of the quantified proteins.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- unknown functione7
- Value
- 3.3%e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e7“cell cytoskeleton (6.7%), other functions (20%) and unknown function (3.3%)”
Claim · model-written summary
The quantified proteins were grouped into five functional groups.
- Taxon
- Vicia faba L.Vicia fabat0
- Outcome
- functional groupse5
- Value
- fivee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e5“The quantified proteins were grouped into five functional groups”
Claim · model-written summary
Chitinase was upregulated under drought.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- droughte0
- Gene
- Chitinasee0
- Outcome
- Chitinasee0
- Direction
- upregulatede0
- Comparator
- control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e0“Chitinase was upregulated under drought, suggesting that it was an important part of the plant defense system”
e1“control plants were regularly irrigated”
Claim · model-written summary
50S ribosomal protein was upregulated under drought.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- droughte0
- Gene
- 50S ribosomal proteine0
- Outcome
- 50S ribosomal proteine0
- Direction
- upregulatede0
- Comparator
- control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e0“50S ribosomal protein was upregulated under drought”
e1“control plants were regularly irrigated”
Claim · model-written summary
The abundance of proteins involved in protein synthesis, including Bet protein and glutamate–glyoxylate aminotransferase, was upregulated under drought stress.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- drought stresse0
- Gene
- Bet protein and glutamate–glyoxylate aminotransferasee0
- Outcome
- abundance of proteins involved in protein synthesise0
- Direction
- upregulatede0
- Comparator
- control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba L.”
e0“The abundance of proteins involved in protein synthesis such as chitinase, Bet protein and glutamate–glyoxylate aminotransferase was upregulated under drought stress”
e1“control plants were regularly irrigated”
The same questions in other papers
drought · physiology: antioxidant enzymes · Vicia faba (faba bean)Results differ
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- no effect
- no effect
1 paper more in this group
See all 5 papers →drought · quality: protein · Vicia faba (faba bean)Positive and negative results
2 in the opposite direction
- negative
- negative
drought · pest · Vicia faba (faba bean)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.