Abstract
The features of rhizogenesis of male-sterile simple hybrids, maternal components of sugar beet heterozosis hybrid and O-types of Darinka variety grown from seeds were investigated. The paper presents the results of studying the main basic nutrient media (B5–A1, B5–A2, B5–A3, B5–A4, B5–A5 and B5–A6) for rhizogenesis, in which the content of macro- and microelements has been reduced by three times, the content of sucrose is reduced by almost 6 times, the amount of agar is reduced by almost five times, while the vitamin РР content is increased by 1.6 times, and the BAP is completely removed. The largest number of rooted microclones was obtained with NAAs by the high indexes of average number of roots and medium indexes of average roots length. To the second, by the number of rooted microclones is assigned, media of 2,4–D, 2,4–DB containing, potassium salt of NAA and IBA were classified, while IAA was characterized by a lower efficiency. The possibilities of regulation of growth and development processes of sugar beet in vitro explants in favor of undifferentiated growth at the stage of proliferation or organogenesis (hemo- and rhizogenesis) have been determined. To improve the quality of rooting of sugar beet plants-regenerants, we have developed the method for dimming agarized nutrient medium with methylene blue at a 0.05% concentration, which made it possible to reduce the inhibitory effect of light on the development of lateral roots. The average index of micro-roots rooting for MS-forms was 81.5 %. The technology of adaptation of micro-roots rooting was developed, in which the plant material at the initial stage of growth had slight morphological changes in the puffer apparatus and stem, but by the end of the vegetation, the plants acquired the appearance characteristic of the donor plants of explants. The clone’s resiliency is reached about 100% in the soil. According to the variability of morphological characteristics in reproduced in vitro sugar beet plants of the MS-forms of the Darynka hybrid, all the plants grown from the regenerants were more, than seed progeny from seed sowing of the same component obtained as a result of the attachment of sterility to the corresponding O-type. It has been found that in vitro cultivation has not weakened, and in some cases even increased the mitotic index of meristem cells of young roots of sugar beet. Most genotypes have reduced the number of pathological mitoses, apparently due to the activation of reparative systems, but did not extend the duration of individual phases of mitosis; without increasing the number of anaphase with bridges and fragments, but changed the number of anaphases with other chromosomal abnormalities. In all variants of the experiment, where activation of growth processes has been observed, the lowest variability of the signs is observed, that is, under the influence of stimulants, which are part of nutrient media, obviously there is unification of cell populations.
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Claims 11
Claim · model-written summary
NAA media produced the largest number of rooted microclones, with high root number and medium root length.
- Taxon
- sugar beet
Beta vulgaris subsp. vulgarist0 - Treatment
- NAAse0
- Outcome
- number of rooted microclonese0
- Direction
- largeste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The largest number of rooted microclones was obtained with NAAs by the high indexes of average number of roots and medium indexes of average roots length.”
t0“sugar beet”
Claim · model-written summary
Media containing 2,4–D, 2,4–DB, potassium salt of NAA and IBA ranked second in rooted microclones.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Outcome
- number of rooted microclonese0
- Direction
- seconde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“To the second, by the number of rooted microclones is assigned, media of 2,4–D, 2,4–DB containing, potassium salt of NAA and IBA were classified”
t0“sugar beet”
Claim · model-written summary
IAA had lower rooting efficiency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Outcome
- efficiencye0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA was characterized by a lower efficiency”
t0“sugar beet”
Claim · model-written summary
Dimming medium with methylene blue reduced light inhibition of lateral root development.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Treatment
- dimming agarized nutrient medium with methylene blue at a 0.05% concentratione0
- Outcome
- inhibitory effect of light on the development of lateral rootse0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dimming agarized nutrient medium with methylene blue at a 0.05% concentration, which made it possible to reduce the inhibitory effect of light on the development of lateral roots”
t0“sugar beet”
Claim · model-written summary
MS forms averaged 81.5% micro-root rooting.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- MS-formse0
- Outcome
- micro-roots rootinge0
- Value
- 81.5 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average index of micro-roots rooting for MS-forms was 81.5 %.”
t0“sugar beet”
Claim · model-written summary
Adapted plant material initially had slight changes in stem and puffer apparatus morphology.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Organ / stage
- puffer apparatus and steme0
- Outcome
- slight morphological changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant material at the initial stage of growth had slight morphological changes in the puffer apparatus and stem”
t0“sugar beet”
Claim · model-written summary
By the end of vegetation, regenerated plants resembled the explant donors.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Organ / stage
- end of the vegetatione0
- Outcome
- appearance characteristic of the donor plants of explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by the end of the vegetation, the plants acquired the appearance characteristic of the donor plants of explants”
t0“sugar beet”
Claim · model-written summary
Clone resilience in soil approached 100%.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Outcome
- clone’s resiliencye0
- Value
- about 100%e0
- Setting
- soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The clone’s resiliency is reached about 100% in the soil.”
t0“sugar beet”
Claim · model-written summary
In vitro cultivation maintained or increased the mitotic index in young root meristems.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Treatment
- in vitro cultivatione0
- Outcome
- mitotic index of meristem cells of young rootse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in vitro cultivation has not weakened, and in some cases even increased the mitotic index of meristem cells of young roots of sugar beet”
Claim · model-written summary
Most genotypes had fewer pathological mitoses.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Outcome
- number of pathological mitosese0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most genotypes have reduced the number of pathological mitoses”
t0“sugar beet”
Claim · model-written summary
Growth activation coincided with the lowest sign variability.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Treatment
- activation of growth processese0
- Outcome
- variability of the signse0
- Direction
- loweste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“where activation of growth processes has been observed, the lowest variability of the signs is observed”
t0“sugar beet”
The same questions in other papers
in vitro · growth · Beta vulgaris (beet)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
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4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.