Abstract
The plastidic glutamine synthetase isoform (GS2) plays a key role in nitrogen (N) assimilation. We introduced TaGS2-2Abpro::TaGS2-2Ab, the favourable allele of TaGS2-2A in the winter wheat (Triticum aestivum) variety Ji5265. Transgenic expression of TaGS2-2Ab significantly increased GS2 abundance and GS activity in leaves. Two consecutive field experiments showed the transgenic lines had higher grain yield, spike number, grain number per spike and 1000-grain weight than did the wild type under both low N and high N conditions. Analysis of N use-related traits showed that transgenic expression of TaGS2-2Ab increased root ability to acquire N, N uptake before and after flowering, remobilization of N to grains and N harvest index. Measurement of chlorophyll content and net photosynthesis rate in flag leaves during grain filling stage revealed that the transgenic lines had prolonged leaf functional duration as compared with the wild type. These results suggest that TaGS2 plays important role in N use, and the favourable allele TaGS2-2Ab is valuable in engineering wheat with improved N use efficiency and grain yield.
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Claims 11
Claim · model-written summary
Transgenic expression increased leaf GS2 abundance and GS activity.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- leavese0
- Treatment
- Transgenic expression of TaGS2-2Abe0
- Gene
- TaGS2-2Abe0
- Outcome
- GS2 abundance and GS activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Transgenic expression of TaGS2-2Ab significantly increased GS2 abundance and GS activity in leaves”
Claim · model-written summary
Transgenic lines had higher grain yield than wild type under low and high N.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic linese0
- Outcome
- grain yielde0
- Direction
- highere0
- Qualifier
- under both low N and high N conditionse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic lines had higher grain yield, spike number, grain number per spike and 1000-grain weight than did the wild type under both low N and high N conditions”
Claim · model-written summary
Transgenic lines had higher spike number than wild type under low and high N.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic linese0
- Outcome
- spike numbere0
- Direction
- highere0
- Qualifier
- under both low N and high N conditionse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic lines had higher grain yield, spike number, grain number per spike and 1000-grain weight than did the wild type under both low N and high N conditions”
Claim · model-written summary
Transgenic lines had higher grain number per spike than wild type under low and high N.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic linese0
- Outcome
- grain number per spikee0
- Direction
- highere0
- Qualifier
- under both low N and high N conditionse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic lines had higher grain yield, spike number, grain number per spike and 1000-grain weight than did the wild type under both low N and high N conditions”
Claim · model-written summary
Transgenic lines had higher 1000-grain weight than wild type under low and high N.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic linese0
- Outcome
- 1000-grain weighte0
- Direction
- highere0
- Qualifier
- under both low N and high N conditionse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic lines had higher grain yield, spike number, grain number per spike and 1000-grain weight than did the wild type under both low N and high N conditions”
Claim · model-written summary
TaGS2-2Ab expression increased root ability to acquire N.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic expression of TaGS2-2Abe0
- Gene
- TaGS2-2Abe0
- Outcome
- root ability to acquire Ne0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic expression of TaGS2-2Ab increased root ability to acquire N, N uptake before and after flowering, remobilization of N to grains and N harvest index”
Claim · model-written summary
TaGS2-2Ab expression increased N uptake before and after flowering.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic expression of TaGS2-2Abe0
- Gene
- TaGS2-2Abe0
- Outcome
- N uptake before and after floweringe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic expression of TaGS2-2Ab increased root ability to acquire N, N uptake before and after flowering, remobilization of N to grains and N harvest index”
Claim · model-written summary
TaGS2-2Ab expression increased remobilization of N to grains.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic expression of TaGS2-2Abe0
- Gene
- TaGS2-2Abe0
- Outcome
- remobilization of N to grainse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic expression of TaGS2-2Ab increased root ability to acquire N, N uptake before and after flowering, remobilization of N to grains and N harvest index”
Claim · model-written summary
TaGS2-2Ab expression increased N harvest index.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic expression of TaGS2-2Abe0
- Gene
- TaGS2-2Abe0
- Outcome
- N harvest indexe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic expression of TaGS2-2Ab increased root ability to acquire N, N uptake before and after flowering, remobilization of N to grains and N harvest index”
Claim · model-written summary
Transgenic lines had longer leaf functional duration during grain filling than wild type.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transgenic linese0
- Outcome
- leaf functional duratione0
- Direction
- prolongede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“transgenic lines had prolonged leaf functional duration as compared with the wild type”
Claim · model-written summary
The authors consider TaGS2-2Ab valuable for improving nitrogen use efficiency and grain yield.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaGS2-2Abe0
- Outcome
- engineering wheat with improved N use efficiency and grain yielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“the favourable allele TaGS2-2Ab is valuable in engineering wheat with improved N use efficiency and grain yield”
The same questions in other papers
transgenic · yield · Triticum aestivum (wheat)Positive and negative results
30 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- no effect
33 papers more in this group
See all 37 papers →transgenic · phenology · Triticum aestivum (wheat)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.