Abstract
Fusarium head blight (FHB), mainly caused by fungus Fusarium graminearum (F. graminearum), is a devastating wheat disease worldwide, leading to reduced yield production and compromised grain quality due to contamination by mycotoxins, such as deoxynivalenol (DON). Manipulating the specific gene expression in microorganisms through RNA interference (RNAi) presents an opportunity for new-generation double-stranded RNA (dsRNA)-based formulations to combat a large number of plant diseases. Here, we applied both spray-induced gene silencing (SIGS) and host-induced gene silencing (HIGS) to target five virulence-related and DON-synthesized genes in F. graminearum, including protein kinase gene Gpmk1, zinc finger protein gene FgChy1, transcription factor FgSR, DON synthesis gene TRI5 and the cell-end marker protein gene FgTeaA, aiming to effectively control FHB in wheat. Direct spraying of individual or combined siRNAs (small interfering RNA) from the fungus showed reduced expression of target genes and suppressed pathogenic symptoms during F. graminearum infection in wheat leaves, with the combination of all five siRNAs demonstrating superior resistance. Furthermore, we generated transgenic wheat lines expressing chimeric RNAi cassettes targeting these five genes, and two independent lines exhibited strong resistance to FHB and Fusarium crown rot, and the reduced DON accumulation. Notably, the HIGS transgenic lines did not adversely impact plant growth and yield traits. Collectively, our findings support that SIGS and HIGS represent effective strategies targeting key pathogenic genes for bolstering disease resistance in crops.
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Claims 6
Claim · model-written summary
Spraying individual or combined fungal siRNAs reduced target-gene expression during Fusarium infection in wheat leaves.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- Direct spraying of individual or combined siRNAse0
- Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Outcome
- expression of target genese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“Direct spraying of individual or combined siRNAs (small interfering RNA) from the fungus showed reduced expression of target genes and suppressed pathogenic symptoms during F. graminearum infection in wheat leaves”
Claim · model-written summary
Spraying individual or combined fungal siRNAs suppressed pathogenic symptoms in infected wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Direct spraying of individual or combined siRNAse0
- Interactor
- F. graminearumFusarium graminearume0pathogen
- Outcome
- pathogenic symptomse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Direct spraying of individual or combined siRNAs (small interfering RNA) from the fungus showed reduced expression of target genes and suppressed pathogenic symptoms during F. graminearum infection in wheat leaves”
Claim · model-written summary
The five-siRNA combination gave superior resistance in wheat.
- Taxon
- wheatTriticum aestivume1
- Treatment
- combination of all five siRNAse0
- Interactor
- F. graminearumFusarium graminearume1pathogen
- Outcome
- resistancee0
- Direction
- superiore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with the combination of all five siRNAs demonstrating superior resistance”
e1“during F. graminearum infection in wheat leaves”
Claim · model-written summary
Two independent HIGS wheat lines showed strong resistance to Fusarium head blight and crown rot.
- Taxon
- wheatTriticum aestivume1
- Material
- two independent linese0
- Treatment
- expressing chimeric RNAi cassettese1
- Outcome
- resistance to FHB and Fusarium crown rote0
- Direction
- stronge0
FusariumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two independent lines exhibited strong resistance to FHB and Fusarium crown rot”
e1“transgenic wheat lines expressing chimeric RNAi cassettes targeting these five genes”
Claim · model-written summary
The HIGS wheat lines had reduced DON accumulation.
- Taxon
- wheatTriticum aestivume1
- Treatment
- expressing chimeric RNAi cassettese1
- Outcome
- DON accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two independent lines exhibited strong resistance to FHB and Fusarium crown rot, and the reduced DON accumulation”
e1“transgenic wheat lines expressing chimeric RNAi cassettes targeting these five genes”
Claim · model-written summary
HIGS wheat lines did not adversely affect growth or yield traits.
- Taxon
- wheatTriticum aestivume1
- Treatment
- HIGS transgenic linese0
- Outcome
- plant growth and yield traitse0
- Direction
- did not adversely impacte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the HIGS transgenic lines did not adversely impact plant growth and yield traits”
e1“transgenic wheat lines”
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