Abstract
Yield in cereals is a function of seed number and weight; both parameters are largely controlled by seed sink strength. The allosteric enzyme ADP-glucose pyrophosphorylase (AGP) plays a key role in regulating starch biosynthesis in cereal seeds and is likely the most important determinant of seed sink strength. Plant AGPs are heterotetrameric, consisting of two large and two small subunits. We transformed wheat (Triticum aestivum L.) with a modified form of the maize (Zea mays L.) Shrunken2 gene (Sh2r6hs), which encodes an altered AGP large subunit. The altered large subunit gives rise to a maize AGP heterotetramer with decreased sensitivity to its negative allosteric effector, orthophosphate, and more stable interactions between large and small subunits. The Sh2r6hs transgene was still functional after five generations in wheat. Developing seeds from Sh2r6hs transgenic wheat exhibited increased AGP activity in the presence of a range of orthophosphate concentrations in vitro. Transgenic Sh2r6hs wheat lines produced on average 38% more seed weight per plant. Total plant biomass was increased by 31% in Sh2r6hs plants. Results indicate increased availability and utilization of resources in response to enhanced seed sink strength, increasing seed yield, and total plant biomass.
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Claims 5
Claim · model-written summary
The Sh2r6hs transgene remained functional after five generations in wheat.
- Taxon
- wheat
Triticum aestivume0 - Gene
- Sh2r6hse0
- Outcome
- transgene was still functionale0
- Value
- five generationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Sh2r6hs transgene was still functional after five generations in wheat.”
Claim · model-written summary
Developing seeds of transgenic wheat showed increased AGP activity across orthophosphate concentrations.
- Taxon
- wheatTriticum aestivume0
- Material
- Sh2r6hs transgenic wheate0
- Organ / stage
- Developing seedse0
- Treatment
- Sh2r6hs transgenice0
- Outcome
- AGP activitye0
- Direction
- increasede0
- Qualifier
- in the presence of a range of orthophosphate concentrations in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Developing seeds from Sh2r6hs transgenic wheat exhibited increased AGP activity in the presence of a range of orthophosphate concentrations in vitro.”
Claim · model-written summary
Sh2r6hs wheat lines produced on average 38% more seed weight per plant.
- Taxon
- wheatTriticum aestivume0
- Material
- Sh2r6hs wheat linese0
- Treatment
- Transgenic Sh2r6hse0
- Outcome
- seed weight per plante0
- Direction
- moree0
- Value
- 38%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic Sh2r6hs wheat lines produced on average 38% more seed weight per plant.”
Claim · model-written summary
Total biomass increased by 31% in Sh2r6hs wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Sh2r6hs plantse0
- Outcome
- Total plant biomasse0
- Direction
- increasede0
- Value
- 31%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Total plant biomass was increased by 31% in Sh2r6hs plants.”
t0“wheat”
Claim · model-written summary
The authors attribute increased seed yield and biomass to resource availability and use following enhanced sink strength.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- seed yield, and total plant biomasse0
- Direction
- increasinge0
- Qualifier
- enhanced seed sink strengthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased availability and utilization of resources in response to enhanced seed sink strength, increasing seed yield, and total plant biomass”
t0“wheat”
The same questions in other papers
transgenic · yield · Triticum aestivum (wheat)Positive and negative results
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- negative
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See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.