Abstract
The WRKY transcription factors play an important role in the regulation of transcriptional reprogramming associated with plant abiotic stress responses. In this study, the WRKY transcription factor MsWRKY11, containing the plant-specific WRKY zinc finger DNA-binding motif, was isolated from alfalfa. The MsWRKY11 gene was detected in all plant tissues (root, stem, leaf, flower, and fruit), with high expression in root and leaf tissues. MsWRKY11 was upregulated in response to a variety of abiotic stresses, including salinity, alkalinity, cold, abscisic acid, and drought. Overexpression of MsWRKY11 in soybean enhanced the salt tolerance at the seedling stage. Transgenic soybean had a better salt-tolerant phenotype, and the hypocotyls were significantly longer than those of wild-type seeds after salt treatment. Furthermore, MsWRKY11 overexpression increased the contents of chlorophyll, proline, soluble sugar, superoxide dismutase, and catalase, but reduced the relative electrical conductivity and the contents of malonaldehyde, H2O2, and O2-. Plant height, pods per plant, seeds per plant, and 100-seed weight of transgenic MsWRKY11 soybean were higher than those of wild-type soybean, especially OX2. Results of the salt experiment showed that MsWRKY11 is involved in salt stress responses, and its overexpression improves salt tolerance in soybean.
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Claims 12
Claim · model-written summary
MsWRKY11 was isolated from alfalfa.
- Taxon
- alfalfa
Medicago sativae0 - Gene
- MsWRKY11e0
- Outcome
- was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The WRKY transcription factor MsWRKY11, containing the plant-specific WRKY zinc finger DNA-binding motif, was isolated from alfalfa.”
Claim · model-written summary
MsWRKY11 was expressed across tested alfalfa tissues, especially roots and leaves.
Not reportedTaxon
Verbatim
e0“The MsWRKY11 gene was detected in all plant tissues (root, stem, leaf, flower, and fruit), with high expression in root and leaf tissues.”
Claim · model-written summary
MsWRKY11 was upregulated in response to salinity, alkalinity, cold, abscisic acid and drought.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- salinity, alkalinity, cold, abscisic acid, and droughte0
- Gene
- MsWRKY11e0
- Outcome
- MsWRKY11e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsWRKY11 was upregulated in response to a variety of abiotic stresses, including salinity, alkalinity, cold, abscisic acid, and drought.”
e1“isolated from alfalfa”
Claim · model-written summary
MsWRKY11 overexpression improved soybean salt tolerance at the seedling stage.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- seedling stagee0
- Treatment
- Overexpression of MsWRKY11e0
- Gene
- MsWRKY11e0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Overexpression of MsWRKY11 in soybean enhanced the salt tolerance at the seedling stage.”
Claim · model-written summary
Salt treated transgenic soybean hypocotyls were significantly longer than wild type.
- Taxon
- soybeanGlycine maxt0
- Treatment
- salt treatmente0
- Outcome
- hypocotylse0
- Direction
- longere0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“the hypocotyls were significantly longer than those of wild-type seeds after salt treatment”
Claim · model-written summary
MsWRKY11 overexpression increased chlorophyll, proline and soluble sugar contents.
- Taxon
- soybeanGlycine maxt0
- Treatment
- MsWRKY11 overexpressione0
- Gene
- MsWRKY11e0
- Outcome
- contents of chlorophyll, proline, soluble sugare0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“MsWRKY11 overexpression increased the contents of chlorophyll, proline, soluble sugar”
Claim · model-written summary
MsWRKY11 overexpression increased superoxide dismutase and catalase contents.
- Taxon
- soybeanGlycine maxt0
- Treatment
- MsWRKY11 overexpressione0
- Gene
- MsWRKY11e0
- Outcome
- superoxide dismutase, and catalasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“MsWRKY11 overexpression increased the contents of chlorophyll, proline, soluble sugar, superoxide dismutase, and catalase”
Claim · model-written summary
MsWRKY11 overexpression reduced relative electrical conductivity.
- Taxon
- soybeanGlycine maxt0
- Outcome
- relative electrical conductivitye0
- Direction
- reducede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“reduced the relative electrical conductivity”
Claim · model-written summary
MsWRKY11 overexpression reduced malonaldehyde, H2O2 and O2- contents.
- Taxon
- soybeanGlycine maxt0
- Outcome
- contents of malonaldehyde, H2O2, and O2-e0
- Direction
- reducede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“reduced the relative electrical conductivity and the contents of malonaldehyde, H2O2, and O2-”
Claim · model-written summary
Transgenic soybean plants were taller than wild type.
- Taxon
- soybeanGlycine maxe0
- Material
- transgenic MsWRKY11 soybeane0
- Outcome
- Plant heighte0
- Direction
- highere0
- Comparator
- wild-type soybeane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Plant height, pods per plant, seeds per plant, and 100-seed weight of transgenic MsWRKY11 soybean were higher than those of wild-type soybean, especially OX2.”
Claim · model-written summary
Transgenic soybean had more pods and seeds per plant.
- Taxon
- soybeanGlycine maxe0
- Material
- transgenic MsWRKY11 soybeane0
- Outcome
- pods per plant, seeds per plante0
- Direction
- highere0
- Comparator
- wild-type soybeane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Plant height, pods per plant, seeds per plant, and 100-seed weight of transgenic MsWRKY11 soybean were higher than those of wild-type soybean, especially OX2.”
Claim · model-written summary
Transgenic soybean had higher 100 seed weight than wild type.
- Taxon
- soybeanGlycine maxe0
- Material
- transgenic MsWRKY11 soybeane0
- Outcome
- 100-seed weighte0
- Direction
- highere0
- Comparator
- wild-type soybeane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“100-seed weight of transgenic MsWRKY11 soybean were higher than those of wild-type soybean, especially OX2.”