Paper · 1981 · Weed Science

EPTC Effects in the Lettuce (Lactuca sativa) Hypocotyl Bioassay for Gibberellins

William W. Donald

7
claims
14
verbatim quotes
5
citations
1
research areas
—
Not open access

Abstract

EPTC ( S -ethyl dipropylthiocarbamate) reduces hypocotyl growth of lettuce [ Lactuca sativa (L.) ‘Grand Rapids’] seedlings in response to gibberellic acid (GA 3 ) in the lettuce hypocotyl bioassay. Doses of 0.01, 0.1, and 1.0 mM EPTC were effective over a range of gibberellin concentrations. Significant reductions in hypocotyl growth in response to all doses of EPTC and GA 3 at 7.68 μg/ml occurred between 12 and 24 h after initial treatment. In a 72-h experiment, pulse treatments of EPTC at 0.01, 0.1, and 1.0 mM plus GA 3 at 7.68 μg/ml for only 1 or 2 h at the beginning reduced the subsequent response of the bioassay when plants were transferred to GA 3 alone for the remaining 71 or 70 h, respectively. Final hypocotyl growth in GA 3 at 7.68 μg/ml was reduced by exposure to EPTC at 0.01, 0.1 and 1.0 mM plus GA 3 at any time during the entire 72 h test period. The interaction between EPTC and GA 3 was not due to herbicide-induced precipitation nor decomposition of GA 3 in the medium. This was demonstrated by separation of [ 14 C]-GA 3 in four thin-layer chromatography solvent systems after incubation in the presence or absence of 1.0 mM EPTC for 48 h. Apparently EPTC can reduce the growth response of sensitive species to exogenous gibberellins in addition to its effects on endogenous gibberellin-like substances or gibberellin biosynthesis.

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

EPTC reduces hypocotyl growth of lettuce seedlings in response to gibberellic acid.

Taxon
Lactuca sativaLactuca sativat0
Treatment
EPTCe1
Compound
gibberellic acide1
Outcome
hypocotyl growthe1
Direction
reducese1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lettuce (Lactuca sativa) Hypocotyl Bioassay”

✓ located verbatim in the source · title, characters 20–63 · show in text ↑
e1

“EPTC ( S -ethyl dipropylthiocarbamate) reduces hypocotyl growth of lettuce [ Lactuca sativa (L.) ‘Grand Rapids’] seedlings in response to gibberellic acid (GA 3 )”

✓ located verbatim in the source · abstract, characters 0–162 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Doses of 0.01, 0.1, and 1.0 mM EPTC were effective over a range of gibberellin concentrations.

Taxon
Lactuca sativaLactuca sativat0
Treatment
Doses of 0.01, 0.1, and 1.0 mM EPTCe2
Outcome
effectivee2
Direction
effectivee2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lettuce (Lactuca sativa) Hypocotyl Bioassay”

✓ located verbatim in the source · title, characters 20–63 · show in text ↑
e2

“Doses of 0.01, 0.1, and 1.0 mM EPTC were effective over a range of gibberellin concentrations”

✓ located verbatim in the source · abstract, characters 198–291 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Significant reductions in lettuce hypocotyl growth in response to all doses of EPTC and GA3 at 7.68 μg/ml occurred between 12 and 24 h after initial treatment.

Taxon
Lactuca sativaLactuca sativat0
Treatment
all doses of EPTC and GA 3 at 7.68 μg/mle3
Outcome
hypocotyl growthe3
Direction
reductionse3
Qualifier
between 12 and 24 h after initial treatmente3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lettuce (Lactuca sativa) Hypocotyl Bioassay”

✓ located verbatim in the source · title, characters 20–63 · show in text ↑
e3

“Significant reductions in hypocotyl growth in response to all doses of EPTC and GA 3 at 7.68 μg/ml occurred between 12 and 24 h after initial treatment”

✓ located verbatim in the source · abstract, characters 293–444 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

In a 72-h experiment, pulse treatments of EPTC plus GA3 for only 1 or 2 h reduced the subsequent response of the bioassay when plants were transferred to GA3 alone.

Taxon
Lactuca sativaLactuca sativat0
Treatment
pulse treatments of EPTC at 0.01, 0.1, and 1.0 mM plus GA 3 at 7.68 μg/ml for only 1 or 2 he4
Outcome
subsequent response of the bioassaye4
Direction
reducede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lettuce (Lactuca sativa) Hypocotyl Bioassay”

✓ located verbatim in the source · title, characters 20–63 · show in text ↑
e4

“In a 72-h experiment, pulse treatments of EPTC at 0.01, 0.1, and 1.0 mM plus GA 3 at 7.68 μg/ml for only 1 or 2 h at the beginning reduced the subsequent response of the bioassay when plants were transferred to GA 3 alone for the remaining 71 or 70 h, respectively”

✓ located verbatim in the source · abstract, characters 446–710 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Final hypocotyl growth in GA3 at 7.68 μg/ml was reduced by exposure to EPTC at 0.01, 0.1 and 1.0 mM plus GA3 at any time during the 72 h test period.

Taxon
Lactuca sativaLactuca sativat0
Treatment
exposure to EPTC at 0.01, 0.1 and 1.0 mMe5
Outcome
Final hypocotyl growthe5
Direction
reducede5
Qualifier
at any time during the entire 72 h test periode5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lettuce (Lactuca sativa) Hypocotyl Bioassay”

✓ located verbatim in the source · title, characters 20–63 · show in text ↑
e5

“Final hypocotyl growth in GA 3 at 7.68 μg/ml was reduced by exposure to EPTC at 0.01, 0.1 and 1.0 mM plus GA 3 at any time during the entire 72 h test period”

✓ located verbatim in the source · abstract, characters 712–869 · show in text ↑
Mechanismobservedoriginal resultmeasured

Claim · model-written summary

The EPTC and GA3 interaction was not due to herbicide-induced precipitation or to decomposition of GA3 in the medium.

Taxon
Lactuca sativaLactuca sativat0
Compound
GA 3e6
Outcome
The interaction between EPTC and GA 3e6
Qualifier
not due to herbicide-induced precipitation nor decomposition of GA 3 in the mediume6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lettuce (Lactuca sativa) Hypocotyl Bioassay”

✓ located verbatim in the source · title, characters 20–63 · show in text ↑
e6

“The interaction between EPTC and GA 3 was not due to herbicide-induced precipitation nor decomposition of GA 3 in the medium”

✓ located verbatim in the source · abstract, characters 871–995 · show in text ↑
Intervention effectauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

EPTC can reduce the growth response of sensitive species to exogenous gibberellins beyond its effects on endogenous gibberellin-like substances or gibberellin biosynthesis.

Taxon
Lactuca sativaLactuca sativat0
Treatment
EPTCe7
Outcome
growth responsee7
Direction
reducee7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lettuce (Lactuca sativa) Hypocotyl Bioassay”

✓ located verbatim in the source · title, characters 20–63 · show in text ↑
e7

“EPTC can reduce the growth response of sensitive species to exogenous gibberellins in addition to its effects on endogenous gibberellin-like substances or gibberellin biosynthesis”

✓ located verbatim in the source · abstract, characters 1179–1358 · show in text ↑

Gibberellic acid · growth · Lactuca sativa (garden lettuce)Results differ

45 papers: 36 positive, 9 negative or no effect

8 papers in the same direction as this paper · 36 in the opposite direction

  1. positive
  2. positive
  3. positive

41 papers more in this group

See all 45 papers →

EPTC · growth · Lactuca sativa (garden lettuce)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.