Abstract
Nitric oxide (NO) is an important signaling molecule with diverse physiological functions in plants. It is therefore important to characterize the downstream genes and signal transduction networks modulated by NO. Here, we identified 1,932 differentially expressed genes (DEGs) responding to NO in upland cotton using high throughput tag sequencing. The results of quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 25 DEGs showed good consistency. Gene Ontology (GO) and KEGG pathway were analyzed to gain a better understanding of these DEGs. We identified 157 DEGs belonging to 36 transcription factor (TF) families and 72 DEGs related to eight plant hormones, among which several TF families and hormones were involved in stress responses. Hydrogen peroxide and malondialdehyde (MDA) contents were increased, as well related genes after treatment with sodium nitroprusside (SNP) (an NO donor), suggesting a role for NO in the plant stress response. Finally, we compared of the current and previous data indicating a massive number of NO-responsive genes at the large-scale transcriptome level. This study evaluated the landscape of NO-responsive genes in cotton and identified the involvement of NO in the stress response. Some of the identified DEGs represent good candidates for further functional analysis in cotton.
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Claims 9
Claim · model-written summary
1,932 genes were differentially expressed in response to NO.
Verbatim
e0“we identified 1,932 differentially expressed genes (DEGs) responding to NO in upland cotton using high throughput tag sequencing.”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
qRT-PCR results for 25 DEGs showed good consistency.
Verbatim
e0“The results of quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 25 DEGs showed good consistency.”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
157 DEGs belonged to 36 transcription factor families.
Verbatim
e0“We identified 157 DEGs belonging to 36 transcription factor (TF) families and 72 DEGs related to eight plant hormones”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
72 DEGs were related to eight plant hormones.
Verbatim
e0“We identified 157 DEGs belonging to 36 transcription factor (TF) families and 72 DEGs related to eight plant hormones”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
Several transcription factor families and hormones were involved in stress responses.
Verbatim
e0“among which several TF families and hormones were involved in stress responses.”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
SNP treatment increased hydrogen peroxide content.
- Taxon
- upland cottont0
- Treatment
- sodium nitroprusside (SNP)e0
- Outcome
- Hydrogen peroxidee0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Hydrogen peroxide and malondialdehyde (MDA) contents were increased, as well related genes after treatment with sodium nitroprusside (SNP) (an NO donor)”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
SNP treatment increased malondialdehyde content.
- Taxon
- upland cottont0
- Treatment
- sodium nitroprusside (SNP)e0
- Outcome
- malondialdehyde (MDA) contentse0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Hydrogen peroxide and malondialdehyde (MDA) contents were increased, as well related genes after treatment with sodium nitroprusside (SNP) (an NO donor)”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
Related genes increased after SNP treatment.
Not reportedComparator
Verbatim
e0“Hydrogen peroxide and malondialdehyde (MDA) contents were increased, as well related genes after treatment with sodium nitroprusside (SNP) (an NO donor)”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
Claim · model-written summary
Comparison with previous data indicated many NO-responsive genes at transcriptome scale.
Verbatim
e0“we compared of the current and previous data indicating a massive number of NO-responsive genes at the large-scale transcriptome level.”
t0“Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum)”
The same questions in other papers
sodium nitroprusside · physiology: oxidative · Gossypium hirsutum (cotton)Positive and negative results
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