Abstract
Flowering time (heading date) in rice (Oryza sativa) is an important agronomic trait that determines yield. The levels of histone H3 lysine 4 trimethylation (H3K4me3) modulated by TRITHORAX-like proteins regulate gene transcription, flowering time and environmental stress responses. However, plant TRITHORAX-like proteins have no known DNA-binding domain, and therefore the mechanism that gives sequence specificity to these proteins remains unclear. Here, we show that the rice TRITHORAX-like protein OsTrx1 is recruited to its target, Early heading date 1 (Ehd1), by the C2H2 zinc finger protein SDG723/OsTrx1/OsSET33 Interaction Protein 1 (SIP1). SIP1 binds to the promoter of Ehd1 and interacts with OsTrx1. Mutations in SIP1 led to a late heading date under long-day and short-day conditions. Defects in OsTrx1 or SIP1 led to reduced H3K4me3 levels at Ehd1, thus reducing Ehd1 expression. Together, our results show that the transcription factor SIP1 interacts with OxTrx1, allowing OsTrx1 to specifically target Ehd1, altering H3K4me3 levels, increasing Ehd1 expression and thereby promoting flowering.
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Claims 8
Claim · model-written summary
SIP1 recruits OsTrx1 to the Ehd1 target.
- Taxon
- rice
Oryza sativae0 - Gene
- SIP1e0
- Outcome
- OsTrx1 is recruited to its target, Early heading date 1 (Ehd1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice TRITHORAX-like protein OsTrx1 is recruited to its target, Early heading date 1 (Ehd1), by the C2H2 zinc finger protein SDG723/OsTrx1/OsSET33 Interaction Protein 1 (SIP1)”
Claim · model-written summary
SIP1 binds the Ehd1 promoter.
- Taxon
- riceOryza sativat0
- Gene
- SIP1e0
- Outcome
- binds to the promoter of Ehd1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“SIP1 binds to the promoter of Ehd1”
Claim · model-written summary
SIP1 interacts with OsTrx1.
- Taxon
- riceOryza sativat0
- Gene
- SIP1e0
- Outcome
- interacts with OsTrx1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“SIP1 binds to the promoter of Ehd1 and interacts with OsTrx1”
Claim · model-written summary
SIP1 mutations delayed heading under long and short days.
- Taxon
- riceOryza sativat0
- Treatment
- Mutations in SIP1e0
- Gene
- SIP1e0
- Outcome
- heading datee0
- Direction
- latee0
- Qualifier
- long-day and short-day conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Mutations in SIP1 led to a late heading date under long-day and short-day conditions.”
Claim · model-written summary
OsTrx1 defects reduced H3K4me3 at Ehd1.
- Taxon
- riceOryza sativat0
- Treatment
- Defects in OsTrx1e0
- Gene
- OsTrx1e0
- Outcome
- H3K4me3 levels at Ehd1e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Defects in OsTrx1 or SIP1 led to reduced H3K4me3 levels at Ehd1”
Claim · model-written summary
SIP1 defects reduced H3K4me3 at Ehd1.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Defects in OsTrx1 or SIP1 led to reduced H3K4me3 levels at Ehd1”
Claim · model-written summary
Reduced H3K4me3 at Ehd1 reduced Ehd1 expression.
- Taxon
- riceOryza sativat0
- Gene
- Ehd1e0
- Outcome
- Ehd1 expressione0
- Direction
- reducinge0
- Qualifier
- reduced H3K4me3 levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“reduced H3K4me3 levels at Ehd1, thus reducing Ehd1 expression”
Claim · model-written summary
SIP1-directed OsTrx1 targeting increases Ehd1 expression and promotes flowering.
- Taxon
- riceOryza sativat0
- Gene
- SIP1e0
- Outcome
- promoting floweringe0
- Direction
- promotinge0
- Qualifier
- increasing Ehd1 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“SIP1 interacts with OxTrx1, allowing OsTrx1 to specifically target Ehd1, altering H3K4me3 levels, increasing Ehd1 expression and thereby promoting flowering”
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loss of function · phenology · Oryza sativa (rice)Positive and negative results
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