Abstract
The flowering period of rice significantly impacts variety adaptability and yield formation. Properly shortening the reproductive period of rice varieties can expand their ecological range without significant yield reduction. Targeted genome editing, like CRISPR/Cas9, is an ideal tool to fine-tune rice growth stages and boost yield synergistically. In this study, we developed a CRISPR/Cas9-mediated multiplex genome-editing vector containing five genes related to three traits, Hd2, Ghd7, and DTH8 (flowering-stage genes), along with the recessive rice blast resistance gene Pi21 and the aromatic gene BADH2. This vector was introduced into the high-quality japonica rice variety in Zhejiang province, Jiahe212 (JH212), resulting in 34 T0 plants with various effective mutations. Among the 17 mutant T1 lines, several displayed diverse flowering dates, but most exhibited undesirable agronomic traits. Notably, three homozygous mutant lines (JH-C15, JH-C18, and JH-C31) showed slightly earlier flowering dates without significant differences in yield-related traits compared to JH212. Through special Hyg and Cas marker selection of T2 plants, we identified seven, six, and two fragrant glutinous plants devoid of transgenic components. These single plants will serve as sib lines of JH212 and potential resources for breeding applications, including maintenance lines for indica–japonica interspecific three-line hybrid rice. In summary, our research lays the foundation for the creation of short-growth-period CMS (cytoplasmic male sterility, CMS) lines, and also provides materials and a theoretical basis for indica–japonica interspecific hybrid rice breeding with wider adaptability.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Several of the 17 mutant T1 rice lines displayed diverse flowering dates.
- Taxon
- Japonica Rice
_by_Augustus_Binu.jpg?width=60)
Oryza sativa Japonica Groupt0 - Material
- mutant T1 linese1
- Outcome
- flowering datese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_by_Augustus_Binu.jpg?width=500)
Verbatim
t0“Japonica Rice Variety Jiahe212”
e1“Among the 17 mutant T1 lines, several displayed diverse flowering dates, but most exhibited undesirable agronomic traits”
Claim · model-written summary
Most of the mutant T1 rice lines exhibited undesirable agronomic traits.
- Taxon
- Japonica RiceOryza sativa Japonica Groupt0
- Material
- mutant T1 linese1
- Outcome
- agronomic traitse1
- Qualifier
- undesirablee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Japonica Rice Variety Jiahe212”
e1“Among the 17 mutant T1 lines, several displayed diverse flowering dates, but most exhibited undesirable agronomic traits”
Claim · model-written summary
Three homozygous edited rice lines showed slightly earlier flowering dates than JH212.
- Taxon
- Japonica RiceOryza sativa Japonica Groupt0
- Material
- JH-C15, JH-C18, and JH-C31e2
- Treatment
- CRISPR/Cas9-mediated multiplex genome-editing vectore3
- Outcome
- flowering datese2
- Direction
- earliere2
- Qualifier
- slightlye2
- Comparator
- JH212e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Japonica Rice Variety Jiahe212”
e2“Notably, three homozygous mutant lines (JH-C15, JH-C18, and JH-C31) showed slightly earlier flowering dates without significant differences in yield-related traits compared to JH212”
e3“we developed a CRISPR/Cas9-mediated multiplex genome-editing vector containing five genes related to three traits, Hd2, Ghd7, and DTH8 (flowering-stage genes), along with the recessive rice blast resistance gene Pi21 and the aromatic gene BADH2”
Claim · model-written summary
The three homozygous edited rice lines showed no significant difference in yield-related traits from JH212.
- Taxon
- Japonica RiceOryza sativa Japonica Groupt0
- Material
- JH-C15, JH-C18, and JH-C31e2
- Treatment
- CRISPR/Cas9-mediated multiplex genome-editing vectore3
- Outcome
- yield-related traitse2
- Direction
- without significant differencese2
- Comparator
- JH212e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Japonica Rice Variety Jiahe212”
e2“Notably, three homozygous mutant lines (JH-C15, JH-C18, and JH-C31) showed slightly earlier flowering dates without significant differences in yield-related traits compared to JH212”
e3“we developed a CRISPR/Cas9-mediated multiplex genome-editing vector containing five genes related to three traits, Hd2, Ghd7, and DTH8 (flowering-stage genes), along with the recessive rice blast resistance gene Pi21 and the aromatic gene BADH2”
Claim · model-written summary
Seven, six and two fragrant glutinous rice plants devoid of transgenic components were identified.
- Taxon
- Japonica RiceOryza sativa Japonica Groupt0
- Outcome
- fragrant glutinous plantse4
- Value
- seven, six, and twoe4
- Qualifier
- devoid of transgenic componentse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Japonica Rice Variety Jiahe212”
e4“we identified seven, six, and two fragrant glutinous plants devoid of transgenic components”
Claim · model-written summary
The selected single plants are potential breeding resources as sib lines of JH212.
Verbatim
t0“Japonica Rice Variety Jiahe212”
e5“these single plants will serve as sib lines of JH212 and potential resources for breeding applications”
The same questions in other papers
loss of function · phenology · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 13 in the opposite direction
- no effect
- negative
- negative
12 papers more in this group
See all 16 papers →loss of function · yield · Oryza sativa (rice)Positive and negative results
71 papers in the same direction as this paper · 62 in the opposite direction
- positive
- positive
- positive
130 papers more in this group
See all 134 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.