Abstract
Rho family GTPase is a fundamental switch that controls many biological processes.Ropl, a pollen specific Rhg family GTPase from giants (Rop) is crucial for pollen tube growth.To further understand the Ropl signaling, I identified a putative effector protein RIC (Rop Interactive protein containing CRIB [dc42/Rac-Interactive Rinding]) and RopGAP CRop GTPase Activating Protein).I showed that RIC I only interacted with active Ropl and was localized to the apical plasma membrane (PM) of the pollen tube.I further showed that different RICs differentially interacted with Ropl.In collaboration with Ying Gu, my study suggests that RICs may be targets o f Rops.RopGAPs contain a CRIB (dc42/Rac Interactive Rinding) motif which is only present in the targets o f Cdc42/Rac/Rop but not in any other Rho GAPs.I showed that the CRIB motif was responsible for the specific activity and strong affinity o f RopGAP I for Ropl, and demonstrated that RopGAP 1 localized to the apical PM in a Ropl and CRIB dependent manner, thus inhibiting pollen tube growth, reducing Ropl activity, and rescuing the Rop 1-induced depolarized growth of pollen tubes.However, R202L mutation mutant o f RopGAP 1 shows the opposite effect of RopGAP 1.Our results suggests that the CRIB motif enhances the activity o f the RopGAP I.
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Claims 9
Claim · model-written summary
RIC I interacted only with active Ropl.
Not reportedTaxonDirection
Verbatim
e0“RIC I only interacted with active Ropl”
Claim · model-written summary
RIC I localized to the pollen tube apical plasma membrane.
Not reportedTaxonDirection
Verbatim
e0“RIC I only interacted with active Ropl and was localized to the apical plasma membrane (PM) of the pollen tube”
Claim · model-written summary
Different RICs interacted differentially with Ropl.
Not reportedTaxonDirection
Verbatim
e0“different RICs differentially interacted with Ropl”
Claim · model-written summary
The CRIB motif accounted for RopGAP I specificity and strong affinity for Ropl.
- Gene
- RopGAP Ie0
- Outcome
- specific activity and strong affinity o f RopGAP I for Rople0
- Qualifier
- CRIB motife0
Not reportedTaxon
Verbatim
e0“the CRIB motif was responsible for the specific activity and strong affinity o f RopGAP I for Ropl”
Claim · model-written summary
RopGAP 1 localized to apical plasma membrane in a Ropl and CRIB dependent manner.
Not reportedTaxonDirection
Verbatim
e0“RopGAP 1 localized to the apical PM in a Ropl and CRIB dependent manner”
Claim · model-written summary
RopGAP 1 inhibited pollen tube growth.
Not reportedTaxonComparator
Verbatim
e0“RopGAP 1 localized to the apical PM in a Ropl and CRIB dependent manner, thus inhibiting pollen tube growth, reducing Ropl activity, and rescuing the Rop 1-induced depolarized growth of pollen tubes”
Claim · model-written summary
RopGAP 1 reduced Ropl activity.
Not reportedTaxonComparator
Verbatim
e0“RopGAP 1 localized to the apical PM in a Ropl and CRIB dependent manner, thus inhibiting pollen tube growth, reducing Ropl activity”
Claim · model-written summary
RopGAP 1 rescued Rop 1-induced depolarized pollen tube growth.
Not reportedTaxonComparator
Verbatim
e0“RopGAP 1 localized to the apical PM in a Ropl and CRIB dependent manner, thus inhibiting pollen tube growth, reducing Ropl activity, and rescuing the Rop 1-induced depolarized growth of pollen tubes”
Claim · model-written summary
The R202L RopGAP 1 mutant showed an opposite effect from RopGAP 1.
Not reportedTaxonDirection
Verbatim
e0“R202L mutation mutant o f RopGAP 1 shows the opposite effect of RopGAP 1”