Abstract
Adverse environmental stresses affect plant growth and crop yields. Sheepgrass (Leymus chinensis (Trin.) Tzvel), an important forage grass that is widely distributed in the east of Eurasia steppe, has high tolerance to extreme low temperature. Many genes that respond to cold stress were identified in sheepgrass by RNA-sequencing, but more detailed studies are needed to dissect the function of those genes. Here, we found that LcFIN2, a sheepgrass freezing-induced protein 2, encoded a chloroplast-targeted protein. Expression of LcFIN2 was upregulated by freezing, chilling, NaCl and abscisic acid (ABA) treatments. Overexpression of LcFIN2 enhanced the survival rate of transgenic Arabidopsis after freezing stress. Importantly, heterologous expression of LcFIN2 in rice exhibited not only higher survival rate but also accumulated various soluble substances and reduced membrane damage in rice under chilling stress. Furthermore, the chlorophyll content, the quantum photochemistry efficiency of photosystem II (ΦPSII), the non-photochemical quenching (NPQ), the net photosynthesis rate (Pn) and the expression of some chloroplast ribosomal-related and photosynthesis-related genes were higher in the transgenic rice under chilling stress. These findings suggested that the LcFIN2 gene could potentially be used to improve low-temperature tolerance in crops.
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Claims 8
Claim · model-written summary
Sheepgrass LcFIN2 encoded a chloroplast-targeted protein.
Not reportedDirection
Verbatim
e0“Here, we found that LcFIN2, a sheepgrass freezing-induced protein 2, encoded a chloroplast-targeted protein.”
Claim · model-written summary
Freezing, chilling, NaCl, and ABA treatments upregulated LcFIN2 expression.
- Taxon
- sheepgrasst0
- Treatment
- freezing, chilling, NaCl and abscisic acid (ABA) treatmentse0
- Gene
- LcFIN2e0
- Outcome
- Expression of LcFIN2e0
- Direction
- upregulatede0
Not reportedComparator
Verbatim
e0“Expression of LcFIN2 was upregulated by freezing, chilling, NaCl and abscisic acid (ABA) treatments.”
t0“sheepgrass”
Claim · model-written summary
LcFIN2 overexpression increased survival of transgenic Arabidopsis after freezing.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Overexpression of LcFIN2e0
- Gene
- LcFIN2e0
- Outcome
- survival ratee0
- Direction
- enhancede0
- Qualifier
- freezing stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of LcFIN2 enhanced the survival rate of transgenic Arabidopsis after freezing stress.”
Claim · model-written summary
Heterologous LcFIN2 expression increased rice survival under chilling.
- Taxon
- rice
Oryza sativae0 - Treatment
- heterologous expression of LcFIN2e0
- Gene
- LcFIN2e0
- Outcome
- survival ratee0
- Direction
- highere0
- Qualifier
- chilling stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of LcFIN2 in rice exhibited not only higher survival rate but also accumulated various soluble substances and reduced membrane damage in rice under chilling stress.”
Claim · model-written summary
Heterologous LcFIN2 rice accumulated more soluble substances under chilling.
- Taxon
- riceOryza sativae0
- Treatment
- heterologous expression of LcFIN2e0
- Gene
- LcFIN2e0
- Outcome
- various soluble substancese0
- Direction
- accumulatede0
- Qualifier
- chilling stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of LcFIN2 in rice exhibited not only higher survival rate but also accumulated various soluble substances and reduced membrane damage in rice under chilling stress.”
Claim · model-written summary
Heterologous LcFIN2 expression reduced rice membrane damage under chilling.
- Taxon
- riceOryza sativae0
- Treatment
- heterologous expression of LcFIN2e0
- Gene
- LcFIN2e0
- Outcome
- membrane damagee0
- Direction
- reducede0
- Qualifier
- chilling stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of LcFIN2 in rice exhibited not only higher survival rate but also accumulated various soluble substances and reduced membrane damage in rice under chilling stress.”
Claim · model-written summary
Transgenic rice under chilling had higher chlorophyll content, PSII photochemistry efficiency, NPQ, and net photosynthesis rate.
- Taxon
- riceOryza sativae0
- Treatment
- transgenic ricee0
- Outcome
- chlorophyll content, the quantum photochemistry efficiency of photosystem II (ΦPSII), the non-photochemical quenching (NPQ), the net photosynthesis rate (Pn)e0
- Direction
- highere0
- Qualifier
- chilling stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the chlorophyll content, the quantum photochemistry efficiency of photosystem II (ΦPSII), the non-photochemical quenching (NPQ), the net photosynthesis rate (Pn) and the expression of some chloroplast ribosomal-related and photosynthesis-related genes were higher in the transgenic rice under chilling stress.”
Claim · model-written summary
Transgenic rice under chilling had higher expression of chloroplast ribosomal and photosynthesis related genes.
- Taxon
- riceOryza sativae0
- Treatment
- transgenic ricee0
- Outcome
- expression of some chloroplast ribosomal-related and photosynthesis-related genese0
- Direction
- highere0
- Qualifier
- chilling stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the chlorophyll content, the quantum photochemistry efficiency of photosystem II (ΦPSII), the non-photochemical quenching (NPQ), the net photosynthesis rate (Pn) and the expression of some chloroplast ribosomal-related and photosynthesis-related genes were higher in the transgenic rice under chilling stress.”
The same questions in other papers
overexpression · physiology: oxidative · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.