Abstract
To investigate the effect of pollination on the fruit quality of ‘Dangshan Su’ pear, ‘Dangshan Su’ was fertilized by the pollen of ‘Wonhwang’ (Pyrus pyrifolia Nakai.) (DW) and ‘Jingbaili’ (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomics, and the quantitative analysis of intermediate metabolites of lignin synthesis was undertaken using targeted metabolomics. The untargeted metabolomics analysis was performed via gas chromatography-mass spectrometry (GC-MS). The targeted metabolomics analysis was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode. The results showed that the metabolite content was significantly different between DW and DJ. Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs), and the sugar, amino acid, and fatty acid content in DW was lower at 63 DAPs. The intermediate metabolites of lignin synthesis were analyzed using the orthogonal partial least squares discriminant analysis (OPLS-DA) model, and the differential metabolites at 47 DAPs were p-coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol. The differential significant metabolite at 63 DAPs was p-coumaric acid. At 47 DAPs and 63 DAPs, the p-coumaric acid level was significantly different, and the p-coumaric acid content was positively correlated with lignin synthesis. The pollination pollen affects the quality of ‘Dangshan Su’ pear fruit through regulation of the sugar, amino acid, and fatty acid content; at the same time, regulating the levels of intermediate metabolites of lignin synthesis, especially the p-coumaric acid content, to affect lignin synthesis ultimately affects the stone cell content and improves the quality of the pears.
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Claims 10
Claim · model-written summary
The two pollen sources produced significantly different fruit metabolite content.
- Taxon
- Pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communist0 - Outcome
- metabolite contente0
- Direction
- differente0
- Comparator
- DW and DJe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“Pear”
e0“metabolite content was significantly different between DW and DJ”
Claim · model-written summary
At 47 days after pollination, DW fruit had higher sugar and amino acid content and lower fatty acid content than DJ fruit.
- Taxon
- PearPyrus communist0
- Material
- DWe0
- Outcome
- sugar and amino acid contente0
- Direction
- highere0
- Qualifier
- 47 days after pollinatione0
- Comparator
- DJe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs)”
Claim · model-written summary
At 47 days after pollination, DW fruit had lower fatty acid content than DJ fruit.
- Taxon
- PearPyrus communist0
- Material
- DWe0
- Outcome
- fatty acid contente0
- Direction
- lowere0
- Qualifier
- 47 days after pollinatione0
- Comparator
- DJe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs)”
Claim · model-written summary
At 63 days after pollination, DW fruit had lower sugar, amino acid and fatty acid content than DJ fruit.
- Taxon
- PearPyrus communist0
- Material
- DWe0
- Outcome
- sugar, amino acid, and fatty acid contente0
- Direction
- lowere0
- Qualifier
- 63 DAPse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“the sugar, amino acid, and fatty acid content in DW was lower at 63 DAPs”
Claim · model-written summary
Five lignin intermediates differed at 47 days after pollination.
- Taxon
- PearPyrus communist0
- Outcome
- p-coumaric acid, ferulic acid, sinapaldehydee0
- Qualifier
- 47 DAPse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“differential metabolites at 47 DAPs were p-coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol”
Claim · model-written summary
Coniferyl alcohol and sinapyl alcohol also differed at 47 days after pollination.
- Taxon
- PearPyrus communist0
- Outcome
- coniferyl alcohol, and sinapyl alcohole0
- Qualifier
- 47 DAPse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“differential metabolites at 47 DAPs were p-coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol”
Claim · model-written summary
P-coumaric acid was a significantly differential metabolite at 63 days after pollination.
- Taxon
- PearPyrus communist0
- Outcome
- p-coumaric acide0
- Qualifier
- 63 DAPse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“The differential significant metabolite at 63 DAPs was p-coumaric acid”
Claim · model-written summary
P-coumaric acid level differed significantly at 47 and 63 days after pollination.
- Taxon
- PearPyrus communist0
- Outcome
- p-coumaric acid levele0
- Direction
- differente0
- Qualifier
- 47 DAPs and 63 DAPse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“At 47 DAPs and 63 DAPs, the p-coumaric acid level was significantly different”
Claim · model-written summary
P-coumaric acid content correlated positively with lignin synthesis.
- Taxon
- PearPyrus communist0
- Compound
- p-coumaric acide0
- Outcome
- lignin synthesise0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“p-coumaric acid content was positively correlated with lignin synthesis”
Claim · model-written summary
The authors infer pollen-source regulation of primary and lignin metabolism affects stone cells and improves pear quality.
- Taxon
- pearPyrus communise0
- Compound
- p-coumaric acide0
- Outcome
- stone cell contente0
- Qualifier
- regulation of the sugar, amino acid, and fatty acid contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pear”
e0“The pollination pollen affects the quality of ‘Dangshan Su’ pear fruit through regulation of the sugar, amino acid, and fatty acid content; at the same time, regulating the levels of intermediate metabolites of lignin synthesis, especially the p-coumaric acid content, to affect lignin synthesis ultimately affects the stone cell content and improves the quality of the pears”