Abstract
A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens was isolated and purified through its susceptible host Vigna unguiculata var. TVu 76. Yield of the purified virus obtained was 7.2 mg/kg. It had an ultraviolet (UV) absorption spectrum at 260 nm as 1.53±0.00 and at 280 nm as 1.45±0.00, which translated into nucleic acid content of 72.9 %. Determination of the molecular weight of the virus coat protein from a plotted graph gave 27 kiloDaltons. When the purified virus was electrophoresed in agarose gel, no secondary band was observed. Absorbance curve at 260 nm was obtained when the purified virus was measured spectrophotometrically. A volume of 16 000μL antiserum was obtained against the virus with concentration of 0.3 mg/ml. The titre value determined for the antiserum raised against the virus was 1:2000 as there was a wider difference between the readings obtained for the healthy and diseased controls. At this dilution, the titre value was two and a half times greater than that of the healthy control. The heterologous: homologous percentage (%) obtained for the isolated virus and TMV (Muguga strain) was 50.0 % when Protein-A sandwich enzyme linked immunosorbent assay (PAS ELISA) was used to determine its serological relationship with the virus and other Tobamovirus strains.Keywords: PAS ELISA, Tobamovirus, coat protein, molecular weight
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Claims 13
Claim · model-written summary
The isolated virus caused mosaic and leaf yellowing symptoms on Mucuna pruriens.
- Taxon
- Mucuna pruriens
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Mucuna pruriense1 - Interactor
- A viruse0pathogen
- Outcome
- mosaic and leaf yellowing symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens was isolated and purified through its susceptible host Vigna unguiculata var. TVu 76.”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
Vigna unguiculata var. TVu 76 was a susceptible host through which the virus was purified.
- Taxon
- Vigna unguiculata
Vigna unguiculatae0 - Material
- TVu 76e0
- Interactor
- A viruse0pathogen
- Outcome
- susceptible hoste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens was isolated and purified through its susceptible host Vigna unguiculata var. TVu 76.”
Claim · model-written summary
The purified virus yield was 7.2 mg/kg.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- Tobamovirus
Tobamovirust0pathogen - Outcome
- Yield of the purified viruse0
- Value
- 7.2 mg/kge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Yield of the purified virus obtained was 7.2 mg/kg.”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
The isolated virus had UV absorption at 260 nm of 1.53±0.00.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- ultraviolet (UV) absorption spectrum at 260 nme0
- Value
- 1.53±0.00e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It had an ultraviolet (UV) absorption spectrum at 260 nm as 1.53±0.00 and at 280 nm as 1.45±0.00”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
The isolated virus had UV absorption at 280 nm of 1.45±0.00.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- at 280 nme0
- Value
- 1.45±0.00e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at 280 nm as 1.45±0.00”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
The virus nucleic acid content was calculated as 72.9%.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- nucleic acid contente0
- Value
- 72.9 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which translated into nucleic acid content of 72.9 %”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
The molecular weight of the virus coat protein was 27 kiloDaltons.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Gene
- virus coat proteine0
- Outcome
- molecular weight of the virus coat proteine0
- Value
- 27 kiloDaltonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Determination of the molecular weight of the virus coat protein from a plotted graph gave 27 kiloDaltons.”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
Agarose gel electrophoresis showed no secondary band for the purified virus.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- no secondary band was observede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the purified virus was electrophoresed in agarose gel, no secondary band was observed.”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
Spectrophotometry showed an absorbance curve at 260 nm for the purified virus.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- Absorbance curve at 260 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Absorbance curve at 260 nm was obtained when the purified virus was measured spectrophotometrically.”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
Antiserum against the virus yielded 16 000μL at 0.3 mg/ml.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- antiserume0
- Value
- 16 000μLe0
- Qualifier
- concentration of 0.3 mg/mle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A volume of 16 000μL antiserum was obtained against the virus with concentration of 0.3 mg/ml.”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
The antiserum titre against the virus was 1:2000.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- titre valuee0
- Value
- 1:2000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The titre value determined for the antiserum raised against the virus was 1:2000”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
At this dilution, the titre was two and a half times the healthy control.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- titre valuee0
- Direction
- greatere0
- Value
- two and a half timese0
- Comparator
- healthy controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At this dilution, the titre value was two and a half times greater than that of the healthy control.”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Claim · model-written summary
PAS ELISA found 50.0% heterologous to homologous serological relationship between the isolate and TMV Muguga strain.
- Taxon
- Mucuna pruriensMucuna pruriense1
- Interactor
- TobamovirusTobamovirust0pathogen
- Outcome
- heterologous: homologous percentage (%)e0
- Value
- 50.0 %e0
- Comparator
- TMV (Muguga strain)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heterologous: homologous percentage (%) obtained for the isolated virus and TMV (Muguga strain) was 50.0 % when Protein-A sandwich enzyme linked immunosorbent assay (PAS ELISA) was used”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”