Claim · model-written summary
Vigna unguiculata var. TVu 76 was a susceptible host through which the virus was purified.
- Taxon
- Vigna unguiculata
Vigna unguiculatae0 - Material
- TVu 76e0
- Interactor
- A viruse0pathogen
- Outcome
- susceptible hoste0
Not reportedGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens was isolated and purified through its susceptible host Vigna unguiculata var. TVu 76.”
Title
Serological detection of close and distant relationships of an isolated Tobamovirus
O.O. Odedara; J.d'A. Hughes; A.C. Odebode; et al. Nigerian Journal of Biotechnology 2018. doi:10.4314/njb.v35i1.14
Other claims from this paper (12)
- Observation The isolated virus caused mosaic and leaf yellowing symptoms on Mucuna pruriens.
- Observation The purified virus yield was 7.2 mg/kg.
- Observation The isolated virus had UV absorption at 260 nm of 1.53±0.00.
- Observation The isolated virus had UV absorption at 280 nm of 1.45±0.00.
- Observation The virus nucleic acid content was calculated as 72.9%.
- Observation The molecular weight of the virus coat protein was 27 kiloDaltons.
- Observation Agarose gel electrophoresis showed no secondary band for the purified virus.
- Observation Spectrophotometry showed an absorbance curve at 260 nm for the purified virus.
- Observation Antiserum against the virus yielded 16 000μL at 0.3 mg/ml.
- Observation The antiserum titre against the virus was 1:2000.
- Observation At this dilution, the titre was two and a half times the healthy control.
- Observation PAS ELISA found 50.0% heterologous to homologous serological relationship between the isolate and TMV Muguga strain.