Claim · model-written summary
PAS ELISA found 50.0% heterologous to homologous serological relationship between the isolate and TMV Muguga strain.
- Taxon
- Mucuna pruriens
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Mucuna pruriense1 - Interactor
- Tobamovirus
Tobamovirust0pathogen - Outcome
- heterologous: homologous percentage (%)e0
- Value
- 50.0 %e0
- Comparator
- TMV (Muguga strain)e0
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“The heterologous: homologous percentage (%) obtained for the isolated virus and TMV (Muguga strain) was 50.0 % when Protein-A sandwich enzyme linked immunosorbent assay (PAS ELISA) was used”
t0“Serological detection of close and distant relationships of an isolated Tobamovirus”
e1“A virus causing mosaic and leaf yellowing symptoms on Mucuna pruriens”
Title
Serological detection of close and distant relationships of an isolated Tobamovirus
O.O. Odedara; J.d'A. Hughes; A.C. Odebode; et al. Nigerian Journal of Biotechnology 2018. doi:10.4314/njb.v35i1.14
Other claims from this paper (12)
- Observation The isolated virus caused mosaic and leaf yellowing symptoms on Mucuna pruriens.
- Observation Vigna unguiculata var. TVu 76 was a susceptible host through which the virus was purified.
- Observation The purified virus yield was 7.2 mg/kg.
- Observation The isolated virus had UV absorption at 260 nm of 1.53±0.00.
- Observation The isolated virus had UV absorption at 280 nm of 1.45±0.00.
- Observation The virus nucleic acid content was calculated as 72.9%.
- Observation The molecular weight of the virus coat protein was 27 kiloDaltons.
- Observation Agarose gel electrophoresis showed no secondary band for the purified virus.
- Observation Spectrophotometry showed an absorbance curve at 260 nm for the purified virus.
- Observation Antiserum against the virus yielded 16 000μL at 0.3 mg/ml.
- Observation The antiserum titre against the virus was 1:2000.
- Observation At this dilution, the titre was two and a half times the healthy control.