Abstract
As a genetically modified crop, transgenic soybean occupies the largest global scale with its food, nutritional, industrial, and pharmaceutical uses.Efficient transformation is a key factor for the improvement of genetically modified soybean. At present, the Agrobacterium-mediated method is primarily used for soybean transformation, but the efficiency of this method is still relatively low (below 5%) compared with rice (above 90%). In this study, we examined the influence of l-glutamine and/or l-asparagine on Agrobacterium-mediated transformation in soybean and explored the probable role in the process of Agrobacterium-mediated transformation. The results showed that when the amino acids l-glutamine and l-asparagine were added separately or together to the culture medium, the shoot induction frequency, elongation rate, and transformation frequency were improved. The combined effects of l-glutamine and l-asparagine were better than those of l-glutamine and l-asparagine alone. The 50 mg/L l-glutamine and 50 mg/L l-asparagine together can enhance the transformation frequency of soybean by attenuating the expression level of GmPRs (GmPR1, GmPR4, GmPR5, and GmPR10) and suppression of the plant defense response. The transgene was successfully transmitted to the T1 generation. This study will be useful in genetic engineering of soybean.
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Claims 5
Claim · model-written summary
Adding l-glutamine and l-asparagine, separately or together, to the culture medium improved shoot induction frequency, elongation rate and transformation frequency in soybean.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- amino acids l-glutamine and l-asparagine were added separately or together to the culture mediume0
- Outcome
- shoot induction frequency, elongation rate, and transformation frequencye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“when the amino acids l-glutamine and l-asparagine were added separately or together to the culture medium, the shoot induction frequency, elongation rate, and transformation frequency were improved”
Claim · model-written summary
Together, l-glutamine and l-asparagine had better effects on soybean transformation than either amino acid alone.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- combined effects of l-glutamine and l-asparaginee0
- Outcome
- effectse0
- Direction
- bettere0
- Comparator
- l-glutamine and l-asparagine alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“The combined effects of l-glutamine and l-asparagine were better than those of l-glutamine and l-asparagine alone”
Claim · model-written summary
50 mg/L l-glutamine together with 50 mg/L l-asparagine enhanced the soybean transformation frequency.
- Taxon
- soybeanGlycine maxe0
- Treatment
- 50 mg/L l-glutamine and 50 mg/L l-asparagine togethere0
- Outcome
- transformation frequencye0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 50 mg/L l-glutamine and 50 mg/L l-asparagine together can enhance the transformation frequency of soybean by attenuating the expression level of GmPRs (GmPR1, GmPR4, GmPR5, and GmPR10) and suppression of the plant defense response”
Claim · model-written summary
The enhancement of soybean transformation frequency by the two amino acids is attributed to attenuated expression of GmPR genes and suppression of the plant defense response.
- Taxon
- soybeanGlycine maxe0
- Gene
- GmPRs (GmPR1, GmPR4, GmPR5, and GmPR10)e0
- Outcome
- transformation frequencye0
- Qualifier
- suppression of the plant defense responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 50 mg/L l-glutamine and 50 mg/L l-asparagine together can enhance the transformation frequency of soybean by attenuating the expression level of GmPRs (GmPR1, GmPR4, GmPR5, and GmPR10) and suppression of the plant defense response”
Claim · model-written summary
The transgene was successfully transmitted to the T1 generation in transformed soybean.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- transgene was successfully transmitted to the T1 generatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“The transgene was successfully transmitted to the T1 generation”
The same questions in other papers
in vitro · growth · Glycine max (soybean)Positive and negative results
37 papers in the same direction as this paper · 14 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
49 papers more in this group
See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.