Abstract
One up-regulated host gene identified previously was found involved in the infection process of Bamboo mosaic virus (BaMV), a single-stranded positive-sense RNA virus. The full length cDNA of this gene was cloned by 5' and 3'-rapid amplification of cDNA ends and found to encode a polypeptide containing a conserved really interesting new gene (RING) domain and a transmembrane domain. The gene might function as an ubiquitin E3 ligase. We designated this protein in Nicotiana benthamiana as ubiquitin E3 ligase containing RING domain 1 (NbUbE3R1). Further characterization by using Tobacco rattle virus-based virus-induced gene silencing (loss-of-function) revealed that increased BaMV accumulation was in both knockdown plants and protoplasts. The gene might have a defensive role in the replication step of BaMV infection. To further inspect the functional role of NbUbE3R1 in BaMV accumulation, NbUbE3R1 was expressed in N. benthamiana plants. The wild-type NbUbE3R1-orange fluorescent protein (NbUbE3R1-OFP), NbUbE3R1/△TM-OFP (removal of the transmembrane domain) and NbUbE3R1/mRING-OFP (mutation at the RING domain, the E2 interaction site) were transiently expressed in plants. NbUbE3R1 and its derivatives all functioned in restricting the accumulation of BaMV. The common feature of these constructs was the intact substrate-interacting domain. Yeast two-hybrid and co-immunoprecipitation experiments used to determine the possible viral-encoded substrate of NbUbE3R1 revealed the replicase of BaMV as the possible substrate. In conclusion, we identified an up-regulated gene, NbUbE3R1 that plays a role in BaMV replication.
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Claims 4
Claim · model-written summary
The cloned host gene encoded a protein with RING and transmembrane domains.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Outcome
- polypeptide containing a conserved really interesting new gene (RING) domain and a transmembrane domaine0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“found to encode a polypeptide containing a conserved really interesting new gene (RING) domain and a transmembrane domain.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbUbE3R1 increased BaMV accumulation in plants and protoplasts.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- knockdowne0
- Interactor
- BaMVe0pathogen
- Outcome
- BaMV accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased BaMV accumulation was in both knockdown plants and protoplasts.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbUbE3R1 and its derivatives restricted BaMV accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbUbE3R1 and its derivativese0
- Interactor
- BaMVe0pathogen
- Gene
- NbUbE3R1e0
- Outcome
- accumulation of BaMVe0
- Direction
- restrictinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbUbE3R1 and its derivatives all functioned in restricting the accumulation of BaMV.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Viral replicase was identified as a possible NbUbE3R1 substrate.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- BaMVe0pathogen
- Gene
- replicase of BaMVe0
- Outcome
- replicase of BaMV as the possible substratee0
- Qualifier
- possible substratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealed the replicase of BaMV as the possible substrate.”
t0“Nicotiana benthamiana”
The same questions in other papers
loss of function · disease · Nicotiana benthamianaPositive and negative results
92 papers in the same direction as this paper · 50 in the opposite direction
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