Abstract
Leaf senescence represents the final stage of leaf development and is regulated by diverse internal and environmental factors. Jasmonates (JAs) have been demonstrated to induce leaf senescence in several species; however, the mechanisms of JA-induced leaf senescence remain largely unknown in tomato plants (Solanum lycopersicum). In the present study, we tested the hypothesis that sedoheptulose-1,7-bisphosphatase (SBPase), an enzyme functioning in the photosynthetic carbon fixation in the Calvin–Benson cycle, was involved in methyl jasmonate (MeJA)- and dark-induced leaf senescence in tomato plants. We found that MeJA and dark induced senescence in detached tomato leaves and concomitantly downregulated the expression of SlSBPASE and reduced SBPase activity. Furthermore, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9)-mediated mutagenesis of SlSBPASE led to senescence-associated characteristics in slsbpase mutant plants, including loss of chlorophyll, repressed photosynthesis, increased membrane ion leakage, and enhanced transcript abundance of senescence-associated genes. Collectively, our data suggest that repression of SBPase by MeJA and dark treatment plays a role in JA- and dark-induced leaf senescence.
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Claims 8
Claim · model-written summary
MeJA induced senescence in detached tomato leaves.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- detached tomato leavese0
- Treatment
- MeJAe0
- Outcome
- senescencee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA and dark induced senescence in detached tomato leaves”
Claim · model-written summary
Darkness induced senescence in detached tomato leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- detached tomato leavese0
- Treatment
- darke0
- Outcome
- senescencee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA and dark induced senescence in detached tomato leaves”
Claim · model-written summary
MeJA and darkness downregulated SlSBPASE expression.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Treatment
- Methyl Jasmonate- and Dark-Inducedt0
- Gene
- SlSBPASEe0
- Outcome
- expression of SlSBPASEe0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“concomitantly downregulated the expression of SlSBPASE”
t0“Methyl Jasmonate- and Dark-Induced Leaf Senescence in Tomato Plants”
Claim · model-written summary
MeJA and darkness reduced SBPase activity.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Treatment
- Methyl Jasmonate- and Dark-Inducedt0
- Gene
- SBPasee0
- Outcome
- SBPase activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced SBPase activity”
t0“Methyl Jasmonate- and Dark-Induced Leaf Senescence in Tomato Plants”
Claim · model-written summary
SlSBPASE mutagenesis produced senescence-associated characteristics.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Material
- slsbpase mutant plantse0
- Treatment
- mutagenesis of SlSBPASEe0
- Gene
- SlSBPASEe0
- Outcome
- senescence-associated characteristicse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutagenesis of SlSBPASE led to senescence-associated characteristics in slsbpase mutant plants”
t0“Tomato Plants”
Claim · model-written summary
SlSBPASE mutants lost chlorophyll and had repressed photosynthesis.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Outcome
- chlorophyll, repressed photosynthesise0
- Direction
- losse0
Not reportedGeneTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including loss of chlorophyll, repressed photosynthesis”
t0“Tomato Plants”
Claim · model-written summary
SlSBPASE mutants had increased membrane ion leakage.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Outcome
- membrane ion leakagee0
- Direction
- increasede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased membrane ion leakage”
t0“Tomato Plants”
Claim · model-written summary
SlSBPASE mutants had enhanced senescence-associated transcript abundance.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Outcome
- transcript abundance of senescence-associated genese0
- Direction
- enhancede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced transcript abundance of senescence-associated genes”
t0“Tomato Plants”
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