Abstract
We investigated the relationship between the expression of lipoxygenase (LOX) genes and the systemin-dependent wound response in tomato (Lycopersicon esculentum) leaves. A polymerase chain reaction-based approach was used to isolate two tomato Lox cDNAs, called TomLoxC and TomLoxD. Both TomLOXC and TomLOXD amino acid sequences possess an N-terminal extension of about 60 residues that were shown by in vitro uptake to function as transit peptides, targeting these proteins into the chloroplast. Within 30 to 50 min following wounding or systemin or methyl jasmonate treatments, the TomLoxD mRNA level increased and reached a maximum between 1 and 2 h. TomLoxC mRNA was not detectable in leaves and was not found following wounding, but it was found in ripening fruits, indicating that the two tomato Lox genes are regulated in different tissues by different processes. The results suggest that the TomLoxD gene is up-regulated in leaves in response to wounding and encodes a chloroplast LOX that may play a role as a component of the octadecanoid defense-signaling pathway.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Two tomato Lox cDNAs, TomLoxC and TomLoxD, were isolated.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- TomLoxC and TomLoxDe0
- Outcome
- two tomato Lox cDNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolate two tomato Lox cDNAs, called TomLoxC and TomLoxD”
Claim · model-written summary
TomLOXC targets the chloroplast through an N-terminal transit peptide.
- Taxon
- tomato (Lycopersicon esculentum)Solanum lycopersicumt0
- Gene
- TomLOXCe0
- Outcome
- targeting these proteins into the chloroplaste0
- Value
- about 60 residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both TomLOXC and TomLOXD amino acid sequences possess an N-terminal extension of about 60 residues that were shown by in vitro uptake to function as transit peptides, targeting these proteins into the chloroplast”
t0“tomato (Lycopersicon esculentum) leaves”
Claim · model-written summary
TomLOXD targets the chloroplast through an N-terminal transit peptide.
- Taxon
- tomato (Lycopersicon esculentum)Solanum lycopersicumt0
- Gene
- TomLOXDe0
- Outcome
- targeting these proteins into the chloroplaste0
- Value
- about 60 residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both TomLOXC and TomLOXD amino acid sequences possess an N-terminal extension of about 60 residues that were shown by in vitro uptake to function as transit peptides, targeting these proteins into the chloroplast”
t0“tomato (Lycopersicon esculentum) leaves”
Claim · model-written summary
Wounding, systemin or methyl jasmonate induced TomLoxD mRNA within 30 to 50 minutes, peaking at 1 to 2 hours.
- Taxon
- tomato (Lycopersicon esculentum)Solanum lycopersicumt0
- Treatment
- wounding or systemin or methyl jasmonate treatmentse0
- Gene
- TomLoxDe0
- Outcome
- TomLoxD mRNA levele0
- Direction
- increasede0
- Value
- 30 to 50 mine0
- Qualifier
- between 1 and 2 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Within 30 to 50 min following wounding or systemin or methyl jasmonate treatments, the TomLoxD mRNA level increased and reached a maximum between 1 and 2 h”
t0“tomato (Lycopersicon esculentum) leaves”
Claim · model-written summary
TomLoxC mRNA was undetectable in leaves, including after wounding.
- Taxon
- tomato (Lycopersicon esculentum)Solanum lycopersicumt0
- Organ / stage
- leavese0
- Gene
- TomLoxCe0
- Outcome
- TomLoxC mRNA was not detectable in leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TomLoxC mRNA was not detectable in leaves and was not found following wounding”
t0“tomato (Lycopersicon esculentum) leaves”
Claim · model-written summary
TomLoxC mRNA was found in ripening fruits.
- Taxon
- tomato (Lycopersicon esculentum)Solanum lycopersicumt0
- Organ / stage
- ripening fruitse0
- Gene
- TomLoxCe0
- Outcome
- found in ripening fruitse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TomLoxC mRNA was not detectable in leaves and was not found following wounding, but it was found in ripening fruits”
t0“tomato (Lycopersicon esculentum) leaves”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
14 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- negative
15 papers more in this group
See all 19 papers →mechanical · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
15 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- no effect
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.