Abstract
Abstract A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks.
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Claims 10
Claim · model-written summary
A full-length tomato LeCDPK1 cDNA was isolated.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- LeCDPK1e0
- Outcome
- full-length cDNA clone (LeCDPK1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated”
Claim · model-written summary
Predicted LeCDPK1 sequence has high similarity to other CDPKs.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Gene
- CDPKe0
- Outcome
- high degree of similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family”
t0“Tomato Plants”
Claim · model-written summary
LeCDPK1 has a putative myristoylation sequence and possible palmitoylation site.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Gene
- LeCDPK1e0
- Outcome
- putative N-terminal myristoylation sequence and presents a possible palmitoylation sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site”
t0“Tomato Plants”
Claim · model-written summary
The translated protein retains CDPK biochemical properties.
- Taxon
- Tomato PlantsSolanum lycopersicumt0
- Gene
- CDPKe0
- Outcome
- biochemical properties of a member of the CDPK familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in vitro translated protein conserves the biochemical properties of a member of the CDPK family”
t0“Tomato Plants”
Claim · model-written summary
CDPK activity was detected in soluble and particulate tomato leaf extracts.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- leavese0
- Gene
- CDPKe0
- Outcome
- CDPK activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CDPK activity was detected in soluble and particulate extracts of tomato leaves”
Claim · model-written summary
Basal LeCDPK1 mRNA was present in tomato roots, stems, leaves and flowers.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- roots, stems, leaves, and flowerse0
- Gene
- LeCDPK1e0
- Outcome
- Basal levels of LeCDPK1 mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants”
Claim · model-written summary
Pathogen elicitors transiently enhanced LeCDPK1 expression in detached tomato leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- detached tomato leavese0
- Treatment
- pathogen elicitorse0
- Gene
- LeCDPK1e0
- Outcome
- expression of LeCDPK1e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors”
Claim · model-written summary
H2O2 transiently enhanced LeCDPK1 expression in detached tomato leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- H2O2e0
- Gene
- LeCDPK1e0
- Outcome
- expression of LeCDPK1e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2”
Claim · model-written summary
Wounding transiently raised LeCDPK1 mRNA locally and in distant tomato leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- mechanical woundinge0
- Gene
- LeCDPK1e0
- Outcome
- LeCDPK1 steady-state mRNA levelse0
- Direction
- increasee0
- Setting
- greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves”
Claim · model-written summary
Wound-induced LeCDPK1 mRNA increase correlated with more soluble CDPK protein and activity.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- woundinge0
- Gene
- LeCDPK1e0
- Outcome
- amount and in the activity of a soluble CDPKe0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves”
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