Paper · 2018 · International scientific and practical conference "Agro-SMART - Smart solutions for agriculture" (Agro-SMART 2018)

In Vitro Reproduction and Ex Vitro Adaptation of Complex Resistant Grape Varieties

A.A. Batukayev; D.О. Palaeva; М.S. Batukaev; et al.

7
claims
22
verbatim quotes
6
citations
3
research areas
✓
Open access · cc-by-nc

Abstract

The research object is complex-resistant grape varieties. Intensively growing green shoots of grapes were taken as initial samples which were cut into single-eye cuttings and then meristems were extracted in laminar boxes. Experiments have shown that regeneration of shoots from extracted apexes occurred at all 6-BAP concentrations. 6-BAP has a positive effect at concentrations of 0,5-1,0 mg/l. To accelerate elongation of microshoots, we studied effects of the gibberellic acid combined with 6-BAP at various concentrations. The combination of 0,5 mg/l 6-BAP + 1,0 mg/l HK showed the best results. This combination accelerated the growth of plant stems, and two weeks after, the shoot was 25-26 mm in size. At the ex-vitro plant adaptation stage, positive lignohumate effects were studied. The ability to increase plant resistance to adverse environmental factors was of special interest. The roots were put into an aqueous solution., The substrate was also watered with this solution immediately after replanting. In order to establish the optimal effect, various drug concentrations were studied. Distilled water was used as a control factor. All concentrations of lignohumate had a positive effect on plants. It is necessary to note that lignohumate increased the leaf area. At concentration of 1,0 g/l, the height of plants ha increased by the 30th day after adaptation.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoot regeneration from extracted apexes of complex-resistant grape varieties occurred at all 6-BAP concentrations.

Taxon
grapee0
Material
complex-resistant grape varietiese0
Organ / stage
extracted apexese1
Treatment
6-BAPe1
Outcome
regeneration of shoots from extracted apexese1

Not reportedComparatorDirection

Verbatim

e0

“The research object is complex-resistant grape varieties”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
e1

“regeneration of shoots from extracted apexes occurred at all 6-BAP concentrations”

✓ located verbatim in the source · abstract, characters 251–332 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

6-BAP at 0,5-1,0 mg/l had a positive effect on grape in vitro cultures.

Taxon
grapee0
Material
complex-resistant grape varietiese0
Treatment
6-BAPe2
Outcome
effecte2
Direction
positivee2
Qualifier
0,5-1,0 mg/le2

Not reportedComparator

Verbatim

e0

“The research object is complex-resistant grape varieties”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
e2

“6-BAP has a positive effect at concentrations of 0,5-1,0 mg/l”

✓ located verbatim in the source · abstract, characters 334–395 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The combination of 0,5 mg/l 6-BAP + 1,0 mg/l HK gave the best results among the gibberellic acid and 6-BAP combinations tested.

Taxon
grapee0
Material
complex-resistant grape varietiese0
Treatment
0,5 mg/l 6-BAP + 1,0 mg/l HKe1
Outcome
best resultse1
Direction
beste1
Comparator
various concentrationse2

Verbatim

e0

“The research object is complex-resistant grape varieties”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
e1

“The combination of 0,5 mg/l 6-BAP + 1,0 mg/l HK showed the best results”

✓ located verbatim in the source · abstract, characters 528–599 · show in text ↑
e2

“we studied effects of the gibberellic acid combined with 6-BAP at various concentrations”

✓ located verbatim in the source · abstract, characters 438–526 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The combination of 0,5 mg/l 6-BAP + 1,0 mg/l HK accelerated stem growth, giving shoots of 25-26 mm after two weeks.

Taxon
grapee0
Material
complex-resistant grape varietiese0
Treatment
0,5 mg/l 6-BAP + 1,0 mg/l HKe1
Outcome
growth of plant stemse3
Direction
acceleratede3
Value
25-26 mme3
Qualifier
two weeks aftere3
Comparator
various concentrationse2

Verbatim

e0

“The research object is complex-resistant grape varieties”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
e1

“The combination of 0,5 mg/l 6-BAP + 1,0 mg/l HK showed the best results”

✓ located verbatim in the source · abstract, characters 528–599 · show in text ↑
e2

“we studied effects of the gibberellic acid combined with 6-BAP at various concentrations”

✓ located verbatim in the source · abstract, characters 438–526 · show in text ↑
e3

“This combination accelerated the growth of plant stems, and two weeks after, the shoot was 25-26 mm in size”

✓ located verbatim in the source · abstract, characters 601–708 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All concentrations of lignohumate had a positive effect on grape plants compared with distilled water.

Taxon
grapee0
Treatment
lignohumatee1
Outcome
effecte1
Direction
positivee1
Comparator
Distilled watere2
Setting
ex-vitro plant adaptation stagee3

Verbatim

e0

“The research object is complex-resistant grape varieties”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
e1

“All concentrations of lignohumate had a positive effect on plants”

✓ located verbatim in the source · abstract, characters 1148–1213 · show in text ↑
e2

“Distilled water was used as a control factor”

✓ located verbatim in the source · abstract, characters 1102–1146 · show in text ↑
e3

“At the ex-vitro plant adaptation stage, positive lignohumate effects were studied”

✓ located verbatim in the source · abstract, characters 710–791 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lignohumate increased the leaf area of grape plants compared with distilled water.

Taxon
grapee0
Treatment
lignohumatee1
Outcome
leaf areae1
Direction
increasede1
Comparator
Distilled watere2

Verbatim

e0

“The research object is complex-resistant grape varieties”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
e1

“It is necessary to note that lignohumate increased the leaf area”

✓ located verbatim in the source · abstract, characters 1215–1279 · show in text ↑
e2

“Distilled water was used as a control factor”

✓ located verbatim in the source · abstract, characters 1102–1146 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lignohumate at 1.0 g/l increased grape plant height by day 30 after adaptation, compared with distilled water.

Taxon
grapee0
Treatment
lignohumatee1
Outcome
height of plantse2
Direction
increasede2
Qualifier
concentration of 1,0 g/le2
Comparator
Distilled watere3

Verbatim

e0

“The research object is complex-resistant grape varieties”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
e1

“All concentrations of lignohumate had a positive effect on plants”

✓ located verbatim in the source · abstract, characters 1148–1213 · show in text ↑
e2

“At concentration of 1,0 g/l, the height of plants ha increased by the 30th day after adaptation”

✓ located verbatim in the source · abstract, characters 1281–1376 · show in text ↑
e3

“Distilled water was used as a control factor”

✓ located verbatim in the source · abstract, characters 1102–1146 · show in text ↑

N-benzyladenine · growth · VitisResults differ

31 papers: 26 positive, 5 negative or no effect

25 papers in the same direction as this paper · 5 in the opposite direction

  1. negative
  2. negative
  3. negative

27 papers more in this group

See all 31 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.