Abstract
Cellulose synthase-like D (CSLD) family was characterized for their expression and functions in Populus trichocarpa. Ten members, PtrCslD1-10, were identified in the P. trichocarpa genome, and they belong to 4 clades by phylogenetic tree analysis. qRT-PCR and promoter:GUS assays in Arabidopsis and P. trichocarpa displayed divergent expression patterns of these 10 PtrCSLD genes in root hairs, root tips, leaves, vascular tissues, xylem and flowers. Among PtrCslD2, PtrCslD4, PtrCslD5, PtrCslD6, and PtrCslD8 that all exhibited expression in root hairs, only PtrCslD5 could restore the root hairless phenotype of the atcsld3 mutant, demonstrating that PtrCslD5 is the functional ortholog of AtCslD3 for root hair formation. Our results suggest more possible functions for other PtrCslD genes in poplar.
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Claims 6
Claim · model-written summary
Ten CSLD members, PtrCslD1-10, were identified in the P. trichocarpa genome.
- Taxon
- P. trichocarpa
Populus trichocarpae0 - Gene
- PtrCslD1-10e0
- Outcome
- Ten memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ten members, PtrCslD1-10, were identified in the P. trichocarpa genome”
Claim · model-written summary
The ten PtrCslD members belong to four clades in phylogenetic tree analysis.
- Taxon
- Populus trichocarpaPopulus trichocarpat0
- Outcome
- 4 cladese0
- Qualifier
- phylogenetic tree analysise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus trichocarpa”
e0“they belong to 4 clades by phylogenetic tree analysis”
Claim · model-written summary
qRT-PCR and promoter:GUS assays showed divergent expression patterns of the 10 PtrCSLD genes in root hairs, root tips, leaves, vascular tissues, xylem and flowers.
- Taxon
- P. trichocarpaPopulus trichocarpae0
- Organ / stage
- root hairs, root tips, leaves, vascular tissues, xylem and flowerse0
- Gene
- these 10 PtrCSLD genese0
- Outcome
- divergent expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“qRT-PCR and promoter:GUS assays in Arabidopsis and P. trichocarpa displayed divergent expression patterns of these 10 PtrCSLD genes in root hairs, root tips, leaves, vascular tissues, xylem and flowers”
Claim · model-written summary
Among the root-hair-expressed PtrCslD genes, only PtrCslD5 restored the root hairless phenotype of the atcsld3 mutant.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- atcsld3 mutante0
- Gene
- PtrCslD5e0
- Outcome
- restore the root hairless phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only PtrCslD5 could restore the root hairless phenotype of the atcsld3 mutant”
e1“in Arabidopsis and P. trichocarpa”
Claim · model-written summary
PtrCslD5 is the functional ortholog of AtCslD3 for root hair formation.
- Taxon
- Populus trichocarpaPopulus trichocarpat0
- Gene
- PtrCslD5e0
- Outcome
- root hair formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus trichocarpa”
e0“demonstrating that PtrCslD5 is the functional ortholog of AtCslD3 for root hair formation”
Claim · model-written summary
The results suggest that other PtrCslD genes may have further functions in poplar.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results suggest more possible functions for other PtrCslD genes in poplar”